Lipolysis occurs extensively in fish muscle post-mortem and is associated with quality deterioration in the frozen tissue. Fish muscle lipolysis is presented in the context of basic fish muscle physiology and lipid composition. Phospholipase A and lipases from fish muscle have been described and characterized. The reaction is usually followed by measurement of free fatty acid production, and several colorimetric assays are available. Processing and storage treatments have been shown to influence the extent and rate of lipolysis in fish muscle; most of the research in this area has been directed at the effects of frozen storage. The interaction of lipolysis and lipid oxidation is a particularly intriguing area of study as triglyceride hydrolysis leads to increased oxidation while phospholipid hydrolysis produces the opposite effect.

Fish muscle lipolysis—a review

An ideal feedstock, kusum (Schleichera triguga) for preparation of biodiesel: Optimization of parameters

Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on lipid profiles in tissue of the Fischer rat.

Ultrasound assisted two-stage biodiesel synthesis from non-edible Schleichera triguga oil using heterogeneous catalyst: Kinetics and thermodynamic analysis.

Rapid and sensitive assay for free fatty acids using rhodamine 6G.

A single step photometric micromethod for determination of fatty acids in lipids in benzene solution, using rhodamine 6G reagent, has been developed. The method eliminates the disadvantage of formation of a biphasic system and is applicable in the presence of glycerides, sterols, epoxy compounds, hydrocarbons and long chain hydroxy compounds such as long chain fatty alcohols. The long chain fatty acids from C12 to C22, both saturated and unsaturated, can be determined with reasonable accuracy in the concentration range of 0.08–0.25 µmole/ml. The method is simple, rapid, and requires relatively inexpensive chemicals.

A simple photometric method for microdetermination of fatty acids in lipids

Chemical methods, chromatography and infrared spectroscopy have been applied to ascertain the location and nature of the cyanogenic compounds in kusum oil. Observations indicate the cyanogenic compounds to be a part of glyceride molecules in which one of the hydroxyl groups of the latter is bonded to the cyanogenic compound through an ether linkage. Chromatographic behavior of the isolated cyanogenic compounds further indicates that at least two glyceride molecules are involved. These glycerides are predominantly esterified with saturated fatty acids.

Studies on some chemical aspects of kusum oil

A micro-titrimetric method for the determination of the oxirane functional group by using quaternary ammonium halide and perchloric acid has been developed. The method can be used for the determination of compounds with about 2 µequivalents of oxirane content.

M. K. Kundu

Papers

A simple photometric method for microdetermination of fatty acids in lipids

Studies on some chemical aspects of kusum oil

A micro-titrimetric method for the determination of the oxirane functional group