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M. L. Williamson

Bio: M. L. Williamson is an academic researcher. The author has contributed to research in topics: Silanization. The author has an hindex of 1, co-authored 1 publications receiving 36 citations.
Topics: Silanization

Papers
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Journal ArticleDOI
TL;DR: The first two non-silanizing coupling methods are simple, inexpensive and non-hazardous compared to the third, more complex method in which an initial Correspondance: to PVS.
Abstract: Several methods for immobilizing anti-T2 mycotoxin monoclonal antibodies on quartz fibers, for use in optical sensor development, have been evaluated with respect to the surface density and stability of the immobilized proteins. the first method activates matrix hydroxyl groups using p-toluenesulfonyl chloride (TSC). the second method activates these groups using p-nitrophenyl chloroformate (NPCF). the third method requires an initial silanization using 3-aminopropyltriethoxysilane (APTES) followed by carrier activation with glutaraldehyde. the activated carrier in all three methods is then reacted with the amino groups of the protein. the first two non-silanizing coupling methods are simple, inexpensive and non-hazardous compared to the third, more complex method in which an initial Correspondance: to PVS

39 citations


Cited by
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Journal ArticleDOI
TL;DR: In this review, the focus is on the occurrence of various types of mycotoxins in food and feed associated with risks to humans and livestock, as well as legislation put forth by various authorities, and on presently practiced detoxification methods.
Abstract: Disease outbreaks due to the consumption of contaminated food and feedstuff are a recurring problem worldwide. The major factor contributing to contamination are microorganisms, especially fungi, which produce low-molecular-weight compounds as secondary metabolites, with confirmed toxic properties referred to as mycotoxins. Several mycotoxins reported to date are cosmopolitan in distribution and incur severe health-associated risks (including cancer and neurological disorders). Hence, creating awareness among consumers, as well as developing new methods for detection and inactivation is of great importance for food safety. In this review, the focus is on the occurrence of various types of mycotoxins in food and feed associated with risks to humans and livestock, as well as legislation put forth by various authorities, and on presently practiced detoxification methods. Brief descriptions on recent developments in mycotoxin detection methodology are also inlcuded. This review is meant to be informative not only for health-conscious consumers but also for experts in the field to pave the way for future research to fill the existing gaps in our knowledge with regard to mycotoxins and food safety.

528 citations

Journal ArticleDOI
TL;DR: 3‐Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics
Abstract: 3‐Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics Sandeep Kumar Vashist,*,†,‡ Edmond Lam, Sabahudin Hrapovic, Keith B. Male, and John H. T. Luong †HSG-IMIT Institut für Mikround Informationstechnik, Georges-Koehler-Allee 103, 79110 Freiburg, Germany ‡Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany National Research Council Canada, Montreal, Quebec H4P 2R2, Canada Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), Department of Chemistry and Analytical, Biological Chemistry Research Facility (ABCRF), University College Cork, Cork, Ireland

250 citations

Journal ArticleDOI
TL;DR: In this paper, a silica gel has been modified with γ-aminopropyltriethoxysilane under varying conditions, controlling the influence of water in the different modification stages.
Abstract: Silica gel has been modified with γ-aminopropyltriethoxysilane under varying conditions, controlling the influence of water in the different modification stages. Diffuse reflectance infrared Fourier-transform (DRIFT) spectra revealed the influence of surface water in the reaction stage and of air humidity in the curing stage. These results were confirmed and refined by 29Si and 13C cross-polarisation magic-angle-spinning nuclear magnetic resonance (CPMASNMR) spectroscopy. Combining the results of both techniques, four modification structures present on the silica surface are proposed, depending on the conditions used.

107 citations

Patent
07 Mar 2002
TL;DR: In this article, a system for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivatives, and mixtures thereof for electrical and optical analysis, especially relating to the presence and activity of ion channels is presented.
Abstract: Systems for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivatives, and mixtures thereof, for electrical and/or optical analysis, especially relating to the presence and/or activity of ion channels.

99 citations

Journal ArticleDOI
01 Jan 2001-Analyst
TL;DR: A theophylline antiserum was covalently immobilized on the surface of a fused silica fiber, modified with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde, and used as a selective and sensitive extraction medium for the immunoaffinity solid phase microextraction (SPME) as mentioned in this paper.
Abstract: A theophylline antiserum was covalently immobilized on the surface of a fused silica fiber, modified with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde, and used as a selective and sensitive extraction medium for the immunoaffinity solid-phase microextraction (SPME) determination of theophylline in serum samples The specificity of the immunoaffinity SPME fiber was first investigated using a fixed concentration of [3H]theophylline together with various amounts of interference, possessing no cross-reactivity with the theophylline antibody No significant non-specific binding was observed The reproducibility of the fiber preparation and the immunoaffinity SPME analysis was also investigated, resulting in a relative standard deviation of 61% for five analyses of the same fiber The antigen–antibody binding isotherm was obtained by analyzing theophylline standards of various concentrations (01–5 ng mL−1) until saturation values were reached Initial binding of theophylline was linear with a r2 = 0968 The cross-reactivity of the theophylline immunoaffinity SPME fiber for the structural analog caffeine was investigated by adding various amounts of caffeine in the presence of theophylline at a saturation concentration and produced a low cross-reactivity value of 01% Finally, spiked serum samples (10 and 50 ng mL−1) were successfully analyzed with an excellent correlation with the standard binding isotherm, thus confirming the performance of the immunoaffinity SPME coating for improved bioanalysis

80 citations