A comparative study of human placental and fetal liver catalase during development.

Background: The present study was undertaken to determine the diagnostic importance of 5’nucleotidase, serum cholinesterase enzyme activity in the patients of liver diseases and also to study the levels of different enzymes like alanine transaminase, aspartate transaminase, total bilirubin, serum albumin , and alkaline phosphatise in different liver diseases(liver cirrhosis, hepatitis, liver abscess, obstructive jaundice). Method: The present study was conducted on 150 subjects, of either sex of various age groups. Study included liver disorder patients (75) attending Medical OPD, patients admitted in medical and surgical wards and patients coming in clinical laboratory, Biochemistry Department J.L.N. Medical College & Hospital, Ajmer. The results of patients were compared with the hundred non liver disease patients of either sex of similar age group. The indices included :5’nucleotidase (by colorimetric method of cambell), serum cholinesterase (by DGKC, colorimetric method) alanine transaminase, aspartate transaminase (by NADH kinetic UV method), total bilirubin (by DMSO method), serum albumin (by bromocresol geen method), and alkaline phosphatise (pnitrophenyl phosphate, kinetic method). ResultThe mean serum 5’Nucleotidase levels, were significantly raise in liver diseases patients in comparison to non liver disease patients (p<0.001) (HS). Mean serum cholinesterase levels were low in in liver disease patients <1979 IU/l comparison to non liver disease patients. Total bilirubin level was raised in 93.7% liver disease patients, Aspartate transaminase was raised in 84.9% patients but also in non liver disease patients (81.4%). Similarly Alanine tranaminase, Total protein and serum albumin level were low in both liver disease patients and non liver disease patients Alkaline phosphatise was raised in both in liver disease and non liver disease patients, so, it was concluded in that serum cholinesterase and 5' nucleotidase are better diagnostic marker then the conventional liver function test that are are raised in other liver disease also.

Estimation of 5’ Nucleotidase and Serum Cholinesterase as Diagnostic Marker to Distinguish Between Various Liver Diseases and Non Liver Diseases

Aim of the study was to find out the role of biochemical markers in diagnosis of alcoholic liver disease and non-alcoholic liver disease and compared these values with healthy subjects of Rajasthan. Total 300 subjects were selected in which 200 were selected as patients group (alcoholic liver disease (ALD) and non-alcoholic liver disease (NALD) group) and 100 were normal healthy subjects. Total 100 alcoholic liver disease patients in which 91 patients were male and 09 patients were female. In nonalcoholic liver disease group 55 males and 45 females are enrolled. Patients were distributed age wise. Serum cholinesterase and serum 5-nucleotidase were estimated by commercially available kit on spectrophotometer. Serum cholinesterase level is 2822.99± 716.51 in ALD patients. In NALD patients, serum cholinesterase level was 4299.95±1014.76 and in control group mean serum cholinesterase level was 6303.99±977.59. Pvalue was statically highly significant. (P≤ 0.0001), as well as serum 5-nucleotidase level was 25.89± 5.77 and 17.70± 4.43 in ALD and NALD respectively. In control group mean level of serum 5-nucleotidase was 9.851± 3.24. (P≤ 0.0001). We conclude that serum cholinesterase level was decreased in both disease group, whereas serum 5-nucleotidase level is increased in both disease groups. Both parameters are liver specific and specific test for alcohol induced or alcoholic liver disease as well as in non-alcoholic liver disease. Both parameters can be used as a marker of liver disease. it was concluded that serum cholinesterase and 5' -nucleotidase are better diagnostic marker then the conventional liver function test that are raised in other liver disease also.

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‘Role of Serum Cholinesterase and 5-Nucleotidase Enzyme Activity in the Diagnosis of Alcoholic and Non-Alcoholic Liver Disease and Compared With Healthy Subjects of Rajasthan’

Characterization of microsomal ATPases from developing human placenta.

Activities and a few properties of alkaline phosphatase and 5’-nucleotidase were compared in the developing human placenta. Both the enzymes were mostly membrane-bound and displayed similar developmental patterns with the highest activities at 24/26 weeks of the placenta. L-Phenylalanine, L-tryptophan and L-leucine were inhibitors of alkaline phosphatase, whereas they had no effect on the 5’-nucleotidase. Alkaline phosphatase from a late stage of gestation appeared to be almost heat-stable. An appreciable part of 5’-nucleotidase was also resistant to heat inactivation and this fraction varied with gestational age of the tissue. For both the enzymes, Vmax changed without alteringK
m values with periods of gestation. Ca2+, Mg2+ and Mn2+ ions stimulated the alkaline phosphatase activity and Hg2+, Zn2+, Cu2+, Ni2+ were inhibitory. 5’-Nucleotidase was not activated by any of these cations. EDTA and Concanavalin A inhibited both the enzymes, although the extent of inhibition was different and also varied with gestation.

M. Mukherjea

Papers

A comparative study of 5'nucleotidase and alkaline phosphatase in human placenta during development