M. Sarathi

Bio: M. Sarathi is an academic researcher from C. Abdul Hakeem College. The author has contributed to research in topics: Shrimp & White spot syndrome. The author has an hindex of 15, co-authored 18 publications receiving 1082 citations.

Oral administration of bacterially expressed VP28dsRNA to protect Penaeus monodon from white spot syndrome virus.

Abstract: We explored the possibility of protecting Penaeus monodon against white spot syndrome virus (WSSV) infection via interference RNA technology by oral administration of bacterially expressed WSSV VP28dsRNA. Shrimp were given dsRNA orally via two methods. In the first method, pellet feed was coated with inactivated bacteria containing overexpressed dsRNA of the WSSV VP28 gene, and in the second method, pellet feed was coated with VP28dsRNA–chitosan complex nanoparticles. The treated shrimp were orally challenged with WSSV by feeding WSSV-infected tissue. The experiment was conducted for 30 days. The dsRNA-treated shrimp challenged with WSSV showed higher survival compared to control shrimp. Sixty-eight percent survival was observed in shrimp fed with feed coated with inactivated bacteria containing dsRNA of the WSSV VP28 gene whereas 37% survival was observed in shrimp fed with VP28dsRNA–chitosan complex nanoparticle–coated feed. The WSSV caused 100% mortality in shrimp fed with pellet feed coated with inactivated bacteria with empty LITMUS38i vector. At the end of the experiment, the tissue samples prepared from randomly selected shrimp that survived were analyzed via reverse transcriptase-polymerase chain reaction and Western blot analysis for WSSV. The samples were negative for WSSV. Based on the present data and the advantages of dsRNA, we believe that oral administration of crude extract of bacterially expressed VP28dsRNA is a potential therapeutic agent against WSSV infection of shrimp.

Nanotechnology in agri-food production: an overview.

Abstract: Nanotechnology is one of the most important tools in modern agriculture, and agri-food nanotechnology is anticipated to become a driving economic force in the near future. Agri-food themes focus on sustainability and protection of agriculturally produced foods, including crops for human consumption and animal feeding. Nanotechnology provides new agrochemical agents and new delivery mechanisms to improve crop productivity, and it promises to reduce pesticide use. Nanotechnology can boost agricultural production, and its applications include: 1) nanoformulations of agrochemicals for applying pesticides and fertilizers for crop improvement; 2) the application of nanosensors/nanobiosensors in crop protection for the identification of diseases and residues of agrochemicals; 3) nanodevices for the genetic manipulation of plants; 4) plant disease diagnostics; 5) animal health, animal breeding, poultry production; and 6) postharvest management. Precision farming techniques could be used to further improve crop yields but not damage soil and water, reduce nitrogen loss due to leaching and emissions, as well as enhance nutrients long-term incorporation by soil microorganisms. Nanotechnology uses include nanoparticle-mediated gene or DNA transfer in plants for the development of insect-resistant varieties, food processing and storage, nanofeed additives, and increased product shelf life. Nanotechnology promises to accelerate the development of biomass-to-fuels production technologies. Experts feel that the potential benefits of nanotechnology for agriculture, food, fisheries, and aquaculture need to be balanced against concerns for the soil, water, and environment and the occupational health of workers. Raising awareness of nanotechnology in the agri-food sector, including feed and food ingredients, intelligent packaging and quick-detection systems, is one of the keys to influencing consumer acceptance. On the basis of only a handful of toxicological studies, concerns have arisen regarding the safety of nanomaterials, and researchers and companies will need to prove that these nanotechnologies do not have more of a negative impact on the environment.

Herbal biomedicines: a new opportunity for aquaculture industry

Abstract: Hormones, antibiotics, vitamins and several other chemicals have been tested in aquaculture operations for various remedies Even though they give positive effects, they cannot be recommended due to their residual and other side effects The alternative herbal bio-medicinal products in the aquacultural operations, that have the characteristics of growth promoting ability and tonic to improve the immune system, act as appetite stimulators They increase consumption, induce maturation, and have antimicrobial capability and also antistress characteristics that will be of immense use in the culture of shrimps and other fin fishes without any environmental and hazardous problems Herbal compounds such as phenolics, polyphenols, alkaloids, quinones, terpenoids, lectines and polypeptides have been shown to be very effective alternatives to antibiotics and other synthetic compounds The present paper is presented after a careful review of more than 50 herbal plants for their biological effects such as growth promotion, immunostimulation, antistress, antibacterial, antifungal, antivirals, appetite stimulators and aphrodisiac