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M.Y. Graham

Bio: M.Y. Graham is an academic researcher from Ohio State University. The author has contributed to research in topics: Elicitor & Glyceollin. The author has an hindex of 12, co-authored 14 publications receiving 1112 citations.

Papers
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Journal ArticleDOI
TL;DR: The soybean cotyledon system, already a model system for defense signal-response and cell-to-cell signaling, may provide a convenient and effective system for functional analysis of plant genes through gene silencing.
Abstract: Isoflavones are thought to play diverse roles in plant-microbe interactions and are also potentially important to human nutrition and medicine. Isoflavone synthase (IFS) is a key enzyme for the formation of the isoflavones. Here, we examined the consequences of RNAi silencing of genes for this enzyme in soybean (Glycine max). Soybean cotyledon tissues were transformed with Agrobacterium rhizogenes carrying an RNAi silencing construct designed to silence expression of both copies of IFS genes. Approximately 50% of emerging roots were transformed with the RNAi construct, and most transformed roots exhibited >95% silencing of isoflavone accumulation. Silencing of IFS was also demonstrated throughout the entire cotyledon (in tissues distal to the transformation site) both by high-performance liquid chromatography analysis of isoflavones and by real-time reverse transcription-PCR. This distal silencing led to a nearly complete suppression of mRNA accumulation for both the IFS1 and IFS2 genes and of isoflavone accumulations induced by wounding or treatment with the cell wall glucan elicitor from Phytophthora sojae. Preformed isoflavone conjugates were not reduced in distal tissues, suggesting little turnover of these stored isoflavone pools. Distal silencing was established within just 5 d of transformation and was highly efficient for a 3- to 4-d period, after which it was no longer apparent in most experiments. Silencing of IFS was effective in at least two genotypes and led to enhanced susceptibility to P. sojae, disrupting both R gene-mediated resistance in roots and nonrace-specific resistance in cotyledon tissues. The soybean cotyledon system, already a model system for defense signal-response and cell-to-cell signaling, may provide a convenient and effective system for functional analysis of plant genes through gene silencing.

214 citations

Journal ArticleDOI
TL;DR: These results suggest that glyceollin biosynthesis may not be solely dependent on the induction of enzymes of early phenylpropanoid and flavonoid metabolism in this organ and resistance to P. m.
Abstract: These results suggest that 1) glyceollin biosynthesis may not be solely dependent on the induction of enzymes of early phenylpropanoid and flavonoid metabolism in this organ and 2) resistance to P. m. f. sp. glycinea race 1, as defined by the Rps 1 c gene in this organ, may reside partly in the rapid release of the isoflavone aglycones from their conjugates and/or in the later steps of glyceollin biosynthesis

176 citations

Journal ArticleDOI
TL;DR: It is reported that PMG wall glucan also induces a rapid and massive accumulation of phenolic polymers in soybean cotyledon cells proximal to the point of elicitor application.
Abstract: Phytophthora megasperma Drechs. f. sp. glycinea Kuan & Erwin (PMG) cell wall glucan has been extensively characterized as an elicitor of the pterocarpan phytoalexins, the glyceollins in soybean (Glycine max L.). Just recently, this glucan was shown to be a potent elicitor of conjugates of the isoflavones, daidzein and genistein as well. Here we report that PMG wall glucan also induces a rapid and massive accumulation of phenolic polymers in soybean cotyledon cells proximal to the point of elicitor application. Deposition of phenolic polymers is over then times that in wounded controls within just 4 hours of elicitor treatment and reaches a maximum by 24 hours. In the same tissues, isoflavone conjugates begin to accumulate at 8 hours and glyceollin at 12 hours. By 24 hours, the total deposition of wall bound phenolics in elicitor-treated tissues is several times greater than the peak glyceollin and isoflavone responses combined. Histochemical stains and quantitation of phenolic residues released after saponification and nitrobenzene or copper oxide oxidation suggest that the covalently linked phenolics include both lignin- and suberin-like polymers as well as simple esterified coumaric and ferulic acid monomers. Accumulations of phenolic polymers are accompanied by equally rapid and massive increases in activity of a specific group of anionic peroxidases. Although increases in peroxidase activity are not strictly limited to cells immediately adjacent to the area of elicitor treatment, the deposition of phenolic polymers is significantly less extensive in distal cells.

167 citations

Journal ArticleDOI
TL;DR: A minimal-wound protocol is employed to clearly separate and characterize the specific contributions of light, wounding, and a wall glucan elicitor preparation from Phytophthora sojae to the regulation of phenylpropanoid defense responses in soybean cotyledon tissues.
Abstract: The spatial and temporal deployment of plant defense responses involves a complex interplay of signal events, often resulting in superimposition of signaling processes. We have employed a minimal-wound protocol to clearly separate and characterize the specific contributions of light, wounding, and a wall glucan elicitor preparation (PWG) from Phytophthora sojae (Kauf. and Gerde.) to the regulation of phenylpropanoid defense responses in soybean (Glycine max L. [Merr.]) cotyledon tissues. The assay also allowed us to clearly reconstitute responses to combinations of these primary signals and to examine the effects of other pathogenesis-related molecules on the responses in a defined manner. Light specifically triggers accumulation of malonylglucosyl conjugates of the 5-hydroxy-isoflavone, genistein, which is normally found in epidermal cells. PWG selectively induces accumulation of conjugates of the 5-deoxy-isoflavone daidzein, the first committed precursor of the phytoalexin glyceollin. Wounding initiates phenolic polymer deposition, a process greatly potentiated by PWG and light. Whereas glutathione selectively enhances light induction of genistein conjugates, methyl jasmonate enhances both light and PWG-induced isoflavone conjugate accumulations. Wound exudate fully activates the cell's capacity (competency) for the phenolic polymer and glyceollin responses to PWG, whereas glutathione partially restores competency, favoring coumestrol and phenolic polymer responses to PWG. Abscisic acid inhibits all induced phenylpropanoid responses.

145 citations

Journal ArticleDOI
TL;DR: Results suggest that in situ release of active fragments from a general resistance elicitor is necessary for HR cell death in soybean roots carrying resistance genes at the Rps 1 locus, and that this cell death response is mediated through accumulations of the 5-deoxyisoflavones.
Abstract: Isoflavonoids are thought to play an important role in soybean (Glycine max) resistance to Phytophthora sojae. This was addressed by silencing two genes for their biosynthesis and a third gene controlling their elicitation. Silencing of genes for isoflavone synthase (IFS) or chalcone reductase (CHR) was achieved in soybean roots through an Agrobacterium rhizogenes-mediated RNAi approach. Effectiveness of silencing was followed both by quantitative reverse transcriptase-polymerase chain reaction and high-performance liquid chromatography analyses. Silencing either IFS or CHR led to a breakdown of Rps-mediated resistance to race 1 of P. sojae in ‘W79’ (Rps 1c) or ‘W82’ (Rps 1k) soybean. Loss of resistance was accompanied by suppression of hypersensitive (HR) cell death in both cultivars and suppression of cell death-associated activation of hydrogen peroxide and peroxidase. The various results suggest that the 5-deoxyisoflavonoids play a critical role in the establishment of cell death and race-specific resistance. The P. sojae cell wall glucan elicitor, a potent elicitor of 5-deoxyisoflavonoids, triggered a cell death response in roots that was also suppressed by silencing either CHR or IFS. Furthermore, silencing of the elicitor-releasing endoglucanase (PR-2) led to a loss of HR cell death and race-specific resistance to P. sojae and also to a loss of isoflavone and cell death responses to cell wall glucan elicitor. Taken together, these results suggest that in situ release of active fragments from a general resistance elicitor (pathogen-associated molecular pattern) is necessary for HR cell death in soybean roots carrying resistance genes at the Rps 1 locus, and that this cell death response is mediated through accumulations of the 5-deoxyisoflavones.

112 citations


Cited by
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Journal ArticleDOI
TL;DR: Progress made on several aspects of elicitor signal transduction leading to production of plant secondary metabolites are summarized, including the integration of multiple signaling pathways into or by transcription factors, as well as the linkage of the above signal components in eliciting network through protein phosphorylation and dephosphorylation.

1,649 citations

Journal ArticleDOI
TL;DR: Many secondary metabolites found in plants have a role in defence against herbivores, pests and pathogens, and a few examples are described and discussed, and some of the problems in determining the precise role(s) of such metabolites highlighted.
Abstract: SUMMARY Many secondary metabolites found in plants have a role in defence against herbivores, pests and pathogens. In this review, a few examples are described and discussed, and some of the problems in determining the precise role(s) of such metabolites highlighted. The role of secondary metabolites in defence may involve deterrence/anti-feedant activity, toxicity or acting as precursors to physical defence systems. Many specialist herbivores and pathogens do not merely circumvent the deterrent or toxic effects of secondary metabolites but actually utilize these compounds as either host recognition cues or nutrients (or both). This is true of both cyanogenic glucosides and glucosinolates, which art discussed in detail as examples of defensive compounds. Their biochemistry is compared and contrasted. An enormous variety of secondary metabolites are derived from shikimic acid or aromatic amino acids, many of which have important roles in defence mechanisms. Several classes of secondary products are ‘induced’ by infection, wounding or herbivory, and examples of these are given. Genetic variation in the speed and extent of such induction may account, at least in part, for the difference between resistant and susceptible varieties. Both salicylates and jasmonates have been implicated as signals in such responses and in many other physiological processes, though their prescise roles and interactions in signalling and development are not fully understood.

1,445 citations

Journal ArticleDOI
TL;DR: This review addresses the problem of response localization and localization of phenolics relative to the sequential development of stages of disease that lead ultimately to resistance expression and initial demonstrations that phenols are significant components of the host.
Abstract: Antibiotic phenols have been found in all plants investigated to date. Some occur constitutively and are thought to function as preformed inhibitors associated with nonhost resistance (84, 94, 128, 134). Others, which are the subject of this review, are formed in response to the ingress of pathogens, and their appearance is considered as part of an active defense response. Since the first suggestions that phenolic intermediates have a role in the active expres­ sion of resistance, an underlying problem in ascertaining that such secondary metabolites are of primary (rather than secondary) importance has been the localization and timing of the host response. In this review we address the problem of response localization and localization of phenolics relative to the sequential development of stages of disease that lead ultimately to resistance expression. Initial demonstrations that phenols are significant components of the host

1,352 citations

Journal ArticleDOI
TL;DR: Different strategies and achievements through the genetic engineering of flavonoid biosynthesis with implication in the industry and the combinatorial biosynthesis in microorganisms by the reconstruction of the pathway to obtain high amounts of specific compounds are discussed.
Abstract: Flavonoids are widely distributed secondary metabolites with different metabolic functions in plants. The elucidation of the biosynthetic pathways, as well as their regulation by MYB, basic helix-loop-helix (bHLH), and WD40-type transcription factors, has allowed metabolic engineering of plants through the manipulation of the different final products with valuable applications. The present review describes the regulation of flavonoid biosynthesis, as well as the biological functions of flavonoids in plants, such as in defense against UV-B radiation and pathogen infection, nodulation, and pollen fertility. In addition, we discuss different strategies and achievements through the genetic engineering of flavonoid biosynthesis with implication in the industry and the combinatorial biosynthesis in microorganisms by the reconstruction of the pathway to obtain high amounts of specific compounds.

1,260 citations

Journal ArticleDOI
TL;DR: The chemistry of ROS (superoxide radical, hydrogen peroxide and hydroxyl radical) is described and the role of ROS in defence responses is demonstrated, and some important issues are considered, such as: which of the ROS is a major building element of the oxidative burst.
Abstract: As plants are confined to the place where they grow, they have to develop a broad range of defence responses to cope with pathogenic infections. The oxidative burst, a rapid, transient, production of huge amounts of reactive oxygen species (ROS), is one of the earliest observable aspects of a plant's defence strategy. First this Review describes the chemistry of ROS (superoxide radical, hydrogen peroxide and hydroxyl radical). Secondly, the role of ROS in defence responses is demonstrated, and some important issues are considered, such as: (1) which of the ROS is a major building element of the oxidative burst; (2) the spatial and temporal regulation of the oxidative burst; and (3) differences in the plant's responses to biotic and abiotic elicitation. Thirdly, the relationships between the oxidative burst and other plant defence responses are indicated. These include: (1) an oxygen consumption, (2) the production of phytoalexins, (3) systemic acquired resistance, (4) immobilization of plant cell wall proteins, (5) changes in membrane permeability and ion fluxes and (6) a putative role in hypersensitive cell death. Wherever possible, the comparisons with models applicable to animal systems are presented. Finally, the question of the origin of ROS in the oxidative burst is considered, and two major hypotheses, (1) the action of NADPH oxidase system analogous to that of animal phagocytes, and (2) the pH-dependent generation of hydrogen peroxide by a cell wall peroxidase, are presented. On the basis of this material, a third 'unifying' hypothesis is presented, where transient changes in the pH of the cell wall compartment are indicated as a core phenomenon in evoking ROS production. Additionally, a germin/oxalate oxidase system which generates H2O2 in response to pathogenic infection is also described.

1,240 citations