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Maciej Masłyk

Bio: Maciej Masłyk is an academic researcher from John Paul II Catholic University of Lublin. The author has contributed to research in topics: Steroid sulfatase & Candida albicans. The author has an hindex of 15, co-authored 50 publications receiving 602 citations. Previous affiliations of Maciej Masłyk include The Catholic University of America & CEU San Pablo University.


Papers
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Journal ArticleDOI
TL;DR: Based on their minimal inhibitory concentrations (MICs) against selected microorganisms, six out of twenty-six compounds showed significant antibacterial activity towards Enterococcus faecalis and the synthesized triazoles show relatively low toxicity against human erythrocytes.
Abstract: A new series of coumarin-1,2,3-triazole conjugates with varied alkyl, phenyl and heterocycle moieties at C-4 of the triazole nucleus were synthesized using a copper(I)-catalysed Huisgen 1,3-dipolar cycloaddition reaction of corresponding O-propargylated coumarin (3) or N-propargylated coumarin (6) with alkyl or aryl azides. Based on their minimal inhibitory concentrations (MICs) against selected microorganisms, six out of twenty-six compounds showed significant antibacterial activity towards Enterococcus faecalis (MIC = 12.5–50 µg/mL). Moreover, the synthesized triazoles show relatively low toxicity against human erythrocytes.

74 citations

Journal ArticleDOI
TL;DR: Bioimaging studies demonstrated that the chemosensing probe 4CBS is an effective fluorescent marker for the detection of Sn2+ in living cells and zebrafish and was able to discriminate between Sn2 + in human cancer cells and Sn2- in normal live cells.
Abstract: An easily accessible colorimetric and fluorescence probe 4-((3-chloro-1,4-dioxo-1,4-dihydronaphthalen-2-yl)amino)benzenesulfonamide (4CBS) was successfully developed for the selective and sensitive detection of Sn2+ in an aqueous solution. The sensing mechanism involves reduction of -C═O into -C-OH groups in 4CBS upon the addition of Sn2+, which initiates the fluorescence turn-on mode. A better linear relationship was achieved between fluorescence intensity and Sn2+ concentration in the range of 0-62.5 μM, with a detection limit (LOD) of 0.115 μM. The binding mechanism of 4CBS for Sn2+ was confirmed by Fourier transform infrared analysis, NMR titrations, and mass (electrospray ionization) spectral analysis. Likewise, the proposed sensing mechanism was supported by quantum chemical calculations. Moreover, bioimaging studies demonstrated that the chemosensing probe 4CBS is an effective fluorescent marker for the detection of Sn2+ in living cells and zebrafish. Significantly, 4CBS was able to discriminate between Sn2+ in human cancer cells and Sn2+ in normal live cells.

64 citations

Journal ArticleDOI
TL;DR: In this article, a naphthoquinone-based chemosensor 2-((3-hydroxyphenyl)amino)-3-(phenylthio)naphthalene-1,4-dione (2HPN) was successfully synthesized for the selective detection of Fe2+.
Abstract: A new naphthoquinone-based chemosensor 2-((3-hydroxyphenyl)amino)-3-(phenylthio)naphthalene-1,4-dione (2HPN) was successfully synthesized for the selective detection of Fe2+. The sensing property o...

59 citations

Journal ArticleDOI
23 Feb 2017-Yeast
TL;DR: The studies revealed that the compound suppressed growth of the cells of reference strains as well as clinical Candida strains, with minimal inhibitory concentration and minimal fungicidal concentration values, thus showing a distinct antivirulent potential.
Abstract: Emodin (1,3,8-trihydroxy-6-methyl-anthraquinone) is a natural secondary plant product, originally isolated from the rhizomes of Rheum palmatum. Many reports show its diuretic, vasorelaxant, antibacterial, antiviral, anti-ulcerogenic, immunosuppressive, hepatoprotective, anti-inflammatory and anticancer potential. Emodin is a pleiotropic molecule capable of interacting with several major molecular targets, e.g. NF-κB, AKT/mTOR and STAT3. The compound can also act as an inhibitor of some protein kinases, with special affinity to protein kinase CK2. The aim of the presented report was to evaluate antifungal properties of emodin and its activity towards CK2 isolated from Candida cells. Our studies revealed that the compound suppressed growth of the cells of reference strains as well as clinical Candida strains, with minimal inhibitory concentration and minimal fungicidal concentration values between 12.5 and 200 μg/mL. Moreover, at a low concentration, the compound was able to effectively stop hyphal formation, thus showing a distinct antivirulent potential. Interestingly, we showed that emodin added to Candida culture inhibited the phosphorylation of many cellular proteins, presumably owing to the inhibition of protein kinase CK2. Notably, the enzyme isolated from the Candida cells was susceptible to emodin with IC50 of 2.8 μg/mL. Indeed, our computational modelling revealed that emodin was able to occupy the ATP-binding pocket of CK2. Copyright © 2017 John Wiley & Sons, Ltd.

54 citations

Journal ArticleDOI
TL;DR: A majority of the synthesized compounds showed the strongest antibacterial properties towards S. aureus, with a high level of selectivity, and none of the tested naphthalene-1,4-dione derivatives exhibited haemolytic activity.

52 citations


Cited by
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Journal Article
TL;DR: It’s time to get used to the idea that there is no such thing as a safe place to die.
Abstract: 它是美国国立医学图书馆(NLM)生产的国际性生物医学文献联机书目数据库,是美国国立医学图书馆MEDLARS系统30多个数据库中最大的一个数据库,是世界上最著名的生物医学数据库之一。其内容相当于3种印刷本检索刊物:《医学索引》(index medicus,IM)、《牙科文献索引》、《国际护理学索引》,收录了1966年以来的70多个国家4300多种期刊的题录和文摘共1100万条记录,

678 citations

05 Oct 2017
TL;DR: This work identifies the first, to the authors' knowledge, fungal cytolytic peptide toxin in the opportunistic pathogen Candida albicans, which directly damages epithelial membranes, triggers a danger response signalling pathway and activates epithelial immunity.
Abstract: Cytolytic proteins and peptide toxins are classical virulence factors of several bacterial pathogens which disrupt epithelial barrier function, damage cells and activate or modulate host immune responses. Such toxins have not been identified previously in human pathogenic fungi. Here we identify the first, to our knowledge, fungal cytolytic peptide toxin in the opportunistic pathogen Candida albicans. This secreted toxin directly damages epithelial membranes, triggers a danger response signalling pathway and activates epithelial immunity. Membrane permeabilization is enhanced by a positive charge at the carboxy terminus of the peptide, which triggers an inward current concomitant with calcium influx. C. albicans strains lacking this toxin do not activate or damage epithelial cells and are avirulent in animal models of mucosal infection. We propose the name ‘Candidalysin’ for this cytolytic peptide toxin; a newly identified, critical molecular determinant of epithelial damage and host recognition of the clinically important fungus, C. albicans.

453 citations

Journal ArticleDOI
TL;DR: The underlying mechanisms of BPA-induced multi-organ toxicity were well summarized, involving the receptor pathways, disruption of neuroendocrine system, inhibition of enzymes, modulation of immune and inflammatory responses, as well as genotoxic and epigenetic mechanisms.

321 citations

Journal ArticleDOI
TL;DR: A better understanding is provided that a particular natural anti-biofilm molecule exhibits a different mode of actions and biofilm inhibitory activity against more than one pathogenic species, which can be exploited further to improve the therapeutic strategy.
Abstract: Pathogenic microorganisms and their chronic pathogenicity are significant concerns in biomedical research. Biofilm-linked persistent infections are not easy to treat due to resident multidrug-resistant microbes. Low efficiency of various treatments and in vivo toxicity of available antibiotics drive the researchers toward the discovery of many effective natural anti-biofilm agents. Natural extracts and natural product-based anti-biofilm agents are more efficient than the chemically synthesized counterparts with lesser side effects. The present review primarily focuses on various natural anti-biofilm agents, i.e., phytochemicals, biosurfactants, antimicrobial peptides, and microbial enzymes along with their sources, mechanism of action via interfering in the quorum-sensing pathways, disruption of extracellular polymeric substance, adhesion mechanism, and their inhibitory concentrations existing in literature so far. This study provides a better understanding that a particular natural anti-biofilm molecule exhibits a different mode of actions and biofilm inhibitory activity against more than one pathogenic species. This information can be exploited further to improve the therapeutic strategy by a combination of more than one natural anti-biofilm compounds from diverse sources.

161 citations

Journal ArticleDOI
TL;DR: An overview of the latest applications of organophosphorus compounds (OPs) that exhibit biological activity can be found in this article, where the authors outline the findings of a literature review on OPs, including anticancer and antiviral agents, bisphosphonates, phosphorus analogues of amino acids and peptides.
Abstract: The purpose of this article is to provide an overview of the latest applications of organophosphorus compounds (OPs) that exhibit biological activity. A large family of OPs have become popular in recent years. The practical application of OPs in modern medicine has been attributed to their unique properties. In this article, the methods used to select these compounds will be emphasized. This paper will first outline the findings of a literature review on OPs, including anticancer and antiviral agents, bisphosphonates, phosphorus analogues of amino acids and peptides, and organophosphorus metal complexes, and secondly, it will classify the compounds according to their biological activity and applications in the treatment of diseases.

160 citations