Marie-Ange Teste

TL;DR: This work provided a set of genes that are suitable reference genes for quantitative gene expression analysis by real-time RT-PCR in yeast biological samples covering a large panel of physiological states.

TL;DR: A highly efficient multiplex genome-editing method in the diatom Phaeodactylum tricornutum, relying on the biolistic delivery of CRISPR-Cas9 ribonucleoproteins coupled with the identification of two endogenous counter-selectable markers, PtUMPS and PtAPT is reported.

TL;DR: Investigating more directly which one, between trehalose and/or the Tps1 protein, may serve yeast cells to withstand exposure to stress supported the idea that TPS1 is endowed with a regulatory function in energy homeostasis, which is essential to withstand adverse conditions and maintain cellular integrity.

TL;DR: It is shown that each of these five genes encodes a protein with α-glucosidase activity on isomaltose, and these genes are renamed IMA1 to IMA5 for IsoMAltase, and illustrated that sequence polymorphisms among this family led to interesting variability of gene expression patterns and of catalytic efficiencies on different substrates, which altogether should account for the absence of functional redundancy for growth on isOMaltose.

TL;DR: Deletion of YPR184w prevents glycogen degradation, whereas overexpression had no effect on the rate of glycogen breakdown, and the transcriptional control of GDB1 (for Glycogen DeBranching gene), is strictly identical to that of other genes involved in glycogen metabolism.