Marie R.G. Kopp

TL;DR: The microfluidics approach represents an attractive platform to investigate the dynamics of compartmentalization in artificial cells in the absence and presence of network structures and shows that the characteristic time scale of phase separation decreases linearly with increasing the volume of the compartment.

TL;DR: A comparison of currently available experimental approaches and the emerging possibilities offered by advances in microfluidic technology for the analysis of therapeutic proteins during manufacturing and formulation is highlighted.

TL;DR: A droplet microfluidic platform to increase the concentration of analytes in solution via reduction of the sample volume under well-defined conditions is developed and the droplet concentrator device, or DroMiCo, can quantify unlabeled proteins in nM concentrations and analyze multicomponent mixtures when coupled with a prefractionation step.

TL;DR: Clear clustering according to cell density was observed by principal component analysis, indicating steady‐state dependent metabolite profiles, and varying levels of nucleotides, nucleotide sugar, and lipid precursors explained most of the variance between the different cell density set points.

TL;DR: The advantages of microfluidic technology for the analysis of several aspects of phase separation, including phase diagrams, dynamics of assembly and disassembly, rheological and surface properties, exchange of materials with the surrounding environment and the coupling between compartmentalization and biochemical reactions are described.