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Marija Stojadinovic

Bio: Marija Stojadinovic is an academic researcher from University of Belgrade. The author has contributed to research in topics: Resveratrol & Whey protein. The author has an hindex of 8, co-authored 17 publications receiving 501 citations.

Papers
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TL;DR: A positive correlation was found between the strength of protein-polyphenol interactions and (a) half time of protein decay in gastric conditions and (b) masking of total antioxidant capacity ofprotein- polyphenol complexes.

199 citations

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TL;DR: The docking analysis shows that EGCG binds in the hydrophobic pocket at the entrance of cleft between α-helical and β-sheet-rich domains and includes residues of aromatic cluster II and the isothermal titration calorimetry results suggest that the binding of E GCG to ALA is enthalpically favorable.

103 citations

Journal ArticleDOI
TL;DR: Sonication-induced structural changes in major whey allergen were not clinically significant in cow's milk allergy patients and Ultrasound can be a safe procedure for dairy processing as it maintains the nutritional value and does not increase allergenic potential of BLG.
Abstract: Scope The aim of this work was to investigate the effects of high-intensity ultrasound (sonication), on the structure and allergenicity of the major cow's milk allergen, beta-lactoglobulin (BLG). Methods and results Structural changes upon sonication of BLG were monitored by circular dichroism spectroscopy, tryptophan emission fluorescence, hydrophobic dye and retinol binding, as well as digestibility and phenol-oxidase cross-linking capacity. Allergenicity was monitored in individual patients’ sera, basophil activation test, and skin prick testing in 41 cow's milk allergy patients. Uncontrolled local temperature changes induced modifications in BLG secondary structure accompanied by formation of dimers, trimers, and oligomers of BLG that were more digestible by pepsin and had reduced retinol binding. Controlled temperature conditions induced changes in secondary structure of BLG without causing formation of oligomers, or changing protein's capacity to bind retinol. Both sonicated forms of BLG had more exposed hydrophobic surfaces than native BLG and underwent facilitated cross-linking reaction with phenol-oxidase. Sonication had a minor effect on IgE-binding properties of BLG. Conclusion Sonication-induced structural changes in major whey allergen were not clinically significant in cow's milk allergy patients. Ultrasound can be a safe procedure for dairy processing as it maintains the nutritional value and does not increase allergenic potential of BLG.

75 citations

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TL;DR: In this article, the potential of laccase to cross-link β-Lactoglobulin (BLG) in the presence of sour cherry (Prunus cerasus L.) extract was investigated.

71 citations

Journal ArticleDOI
TL;DR: This study provides further evidence in support of the proposed mechanism by which EGCG interactions with the food allergens contribute to its diverse biological activities and may impair antigen uptake by antigen-presenting cells.

71 citations


Cited by
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Journal ArticleDOI
TL;DR: In this article, the effects of protein-phenolic interactions under various conditions on protein and phenolic compound's structure and functionality are described, including temperature, pH, protein type and concentration, and the type and structure of phenolic compounds.

856 citations

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TL;DR: The interactions between phenolic compounds and lipids, carbohydrates and proteins and their impact on polyphenol activity are reviewed.

763 citations

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TL;DR: Increased knowledge of the factors affecting polyphenol bioavailability, including dietary factors, is paramount and polyphenols may act synergistically due to their influence on efflux transporters such as p-glycoprotein.
Abstract: While many epidemiological studies have associated the consumption of polyphenols within fruits and vegetables with a decreased risk of developing several chronic diseases, intervention studies have generally not confirmed these beneficial effects. The reasons for this discrepancy are not fully understood but include potential differences in dosing, interaction with the food matrix, and differences in polyphenol bioavailability. In addition to endogenous factors such as microbiota and digestive enzymes, the food matrix can also considerably affect bioaccessibility, uptake, and further metabolism of polyphenols. While dietary fiber (such as hemicellulose), divalent minerals, and viscous and protein-rich meals are likely to cause detrimental effects on polyphenol bioaccessibility, digestible carbohydrates, dietary lipids (especially for hydrophobic polyphenols, e.g., curcumin), and additional antioxidants may enhance polyphenol availability. Following epithelial uptake, polyphenols such as flavonoids may reduce phase II metabolism and excretion, enhancing polyphenol bioavailability. Furthermore, polyphenols may act synergistically due to their influence on efflux transporters such as p-glycoprotein. In order to understand polyphenol bioactivity, increased knowledge of the factors affecting polyphenol bioavailability, including dietary factors, is paramount.

396 citations

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TL;DR: The overall conclusion drawn is that processing does not completely abolish the allergenic potential of allergens, and there remains a need to develop robust and integrated methods for the risk assessment of food allergenicity.

376 citations

Journal ArticleDOI
TL;DR: The subject matter is divided into the following sections: the role of ras oncogenes and bladder carcinoma and the initial steps in cancer dissemination of rat bladder carcinomas and the last two sections contain a mixture of topics and on the whole are of little interest.
Abstract: proceedings, in not being up-to-date. There are almost no references to papers later than 1990, except for some self-citations which extend to 1991. In addition to well known major contributors to the field of metastasis, research participants were also drawn from laboratories in Continental Europe, which are not frequently represented at Symposia of this type. Some of the papers are general overviews, while others report the Authors own studies in detail. On the whole the various contributions are rather disparate, but occasionally authors compare and contrast a related phenomenon. An example is the role of the CD-44, a transmembrane protein involved in lymphocyte transport, for which the group of Herrlich (Karlsruhe, Germany) have obtained convincing evidence of a role in metastasis. On the other hand, I.R. Hart (London, UK) described studies involving a panel of melanoma cells in which no evidence for a direct involvement of CD-44 in metastasis was found. The subject matter is divided into the following sections: The first section has papers dealing with the role of ras oncogenes and bladder carcinoma and the initial steps in cancer dissemination of rat bladder carcinoma. These raise questions without providing any answers. The second section is concerned principally with cell adhesion and highlights complexity and conflicting results. In the third section the discussion of angiogenesis is disappointing and work is reported from investigators who are not prominent in the area. There is, however, a very valuable chapter from L. Weiss (Buffalo, New York). He analyses, in detail, a mechanism for the destruction of cancer cells in the microvasculature based on deformation. Weiss' data for the destruction of cancer cells when trapped in capillaries has long been a key observation in the field of blood-borne metastatic spread. It leads to the concept of metastatic inefficiency which is so evident when the relative infrequency of metastasis is compared with the number of cancer cells shed into the circulation. Weiss' suggestion as to how this destruction occurs is compelling and he provides most interesting new data which indicates that treatment of cells with doxorubicin increases biomechanical destruction. In the section on melanoma there are two rather unconvincing contributions on metastasis-associated surface molecules, in addition to the critical analysis of the role of CD-44 to which reference has already been made. The last two sections contain a mixture of topics and on the whole are of little interest. Immunotherapy with autologous tumour 'vaccines' …

329 citations