M
Mario Amendola
Researcher at Université Paris-Saclay
Publications - 40
Citations - 6575
Mario Amendola is an academic researcher from Université Paris-Saclay. The author has contributed to research in topics: Genome editing & CRISPR. The author has an hindex of 23, co-authored 37 publications receiving 5417 citations. Previous affiliations of Mario Amendola include Netherlands Cancer Institute & Vita-Salute San Raffaele University.
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Journal ArticleDOI
Easy quantitative assessment of genome editing by sequence trace decomposition
TL;DR: TIDE, a method that requires only a pair of PCR reactions and two standard capillary sequencing runs to identify the major induced mutations in the projected editing site and accurately determines their frequency in a cell population, is presented.
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Single-Cell Dynamics of Genome-Nuclear Lamina Interactions
Jop Kind,Ludo Pagie,Havva Ortabozkoyun,Shelagh Boyle,Sandra S. de Vries,Hans Janssen,Mario Amendola,Leisha D. Nolen,Wendy A. Bickmore,Bas van Steensel +9 more
TL;DR: The H3K9 methyltransferase G9a is identified as a regulator of NL contacts and contact of individual LADs with the NL is linked to transcriptional repression and H 3K9 dimethylation in single cells.
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The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension
Judith H.I. Haarhuis,Robin H. van der Weide,Vincent A. Blomen,J. Omar Yáñez-Cuna,Mario Amendola,Marjon S. van Ruiten,Peter H.L. Krijger,Hans Teunissen,René H. Medema,Bas van Steensel,Thijn R. Brummelkamp,Elzo de Wit,Benjamin D. Rowland +12 more
TL;DR: It is shown here that chromatin loop size can be increased and that the duration with which cohesin embraces DNA determines the degree to which loops are enlarged, and that TADs reflect polyclonal collections of loops in the making.
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Endogenous microRNA can be broadly exploited to regulate transgene expression according to tissue, lineage and differentiation state
Brian D. Brown,Bernhard Gentner,Alessio Cantore,Silvia Colleoni,Mario Amendola,Anna Zingale,Alessia Baccarini,Giovanna Lazzari,Cesare Galli,Luigi Naldini +9 more
TL;DR: These vectors sharply segregated transgene expression between closely related states of therapeutically relevant cells, including dendritic cells, hematopoietic and embryonic stem cells, and their progeny, allowing positive/negative selection according to the cells' differentiation state.
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Forebrain ependymal cells are Notch-dependent and generate neuroblasts and astrocytes after stroke
Marie Carlén,Marie Carlén,Konstantinos Meletis,Konstantinos Meletis,Christian Göritz,Vladimer Darsalia,Emma Evergren,Kenji Tanigaki,Mario Amendola,Fanie Barnabé-Heider,Maggie S. Y. Yeung,Luigi Naldini,Tasuku Honjo,Zaal Kokaia,Oleg Shupliakov,Robert Cassidy,Olle Lindvall,Jonas Frisén +17 more
TL;DR: Although ependymal cells act as primary cells in the neural lineage to produce neurons and glial cells after stroke, they do not fulfill defining criteria for stem cells under these conditions and instead serve as a reservoir that is recruited by injury.