M
Mark A. Knepper
Researcher at National Institutes of Health
Publications - 498
Citations - 37218
Mark A. Knepper is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Vasopressin & Kidney. The author has an hindex of 97, co-authored 480 publications receiving 34737 citations. Previous affiliations of Mark A. Knepper include University of Texas Medical Branch & Johns Hopkins University School of Medicine.
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Journal ArticleDOI
Identification and proteomic profiling of exosomes in human urine
TL;DR: The results indicate that exosome isolation may provide an efficient first step in biomarker discovery in urine and identify numerous protein components of MVBs and of the endosomal pathway in general.
Journal ArticleDOI
Vasopressin increases water permeability of kidney collecting duct by inducing translocation of aquaporin-CD water channels to plasma membrane
Søren Nielsen,Chung Lin Chou,David Marples,Erik Ilsø Christensen,Bellamkonda K. Kishore,Mark A. Knepper +5 more
TL;DR: It is concluded that vasopressin increases the water permeability of collecting duct cells by inducing a reversible translocation of AQP-CD water channels from IVs to the APM.
Journal ArticleDOI
Aldosterone-mediated regulation of ENaC α, β, and γ subunit proteins in rat kidney
TL;DR: At the protein level, the response of ENaC to aldosterone stimulation is heterogenous, with both quantitative and qualitative changes that can explain observed increases in ENac-mediated sodium transport.
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Large-Scale Proteomics and Phosphoproteomics of Urinary Exosomes
Patricia A. Gonzales,Trairak Pisitkun,Jason D. Hoffert,Dmitry Tchapyjnikov,Robert A. Star,Robert Kleta,Nam Sun Wang,Mark A. Knepper +7 more
TL;DR: Overall, the analysis identified 1132 proteins unambiguously, including 177 that are represented on the Online Mendelian Inheritance in Man database of disease-related genes, suggesting that exosome analysis is a potential approach to discover urinary biomarkers.
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Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery
Hua Zhou,Peter S.T. Yuen,Trairak Pisitkun,Patricia A. Gonzales,Hideo Yasuda,James W. Dear,Peter Gross,Mark A. Knepper,Robert A. Star +8 more
TL;DR: It is concluded that protease inhibitors are essential for preservation, and storage at -80 degrees C with extensive vortexing after thawing maximizes the recovery of urinary exosomes, suggesting minimal protein degradation in the urinary tract/bladder.