Mark P. Brynildsen

TL;DR: A synthetic pathway is engineered in Escherichia coli and the production of 1-butanol is demonstrated from this non-native user-friendly host, showing promise for using E. coli for 1- butanol production.

TL;DR: It is shown that specific metabolic stimuli enable the killing of both Gram-negative and Gram-positive bacterial persisters with aminoglycosides, and this approach can improve the treatment of chronic infections in a mouse urinary tract infection model.

TL;DR: Scientists working on the response of bacteria to antibiotics define antibiotic persistence and provide practical guidance on how to study bacterial persister cells, and provide a guide to measuring persistence.

TL;DR: It is shown that ROS production can be predictably enhanced in Escherichia coli, increasing the bacteria's susceptibility to oxidative attack and establishing a systems-based method to tune ROS production in bacteria.

TL;DR: It is discovered that carbon source transitions stimulate the formation of fluoroquinolone persisters in Escherichia coli and this pathway spans from initial stress to antibiotic target and demonstrates that TA behavior can be exhibited by a metabolite-enzyme interaction (ppGpp-SpoT), in contrast to classical TA systems that involve only protein and/or RNA.