Molecular cloning and expression of the fas ligand, a novel member of the tumor necrosis factor family

Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.

Inflammaging and anti-inflammaging: A systemic perspective on aging and longevity emerged from studies in humans

Two molecular mechanisms of T cell-mediated cytotoxicity, one perforin-based, the other Fas-based, have been demonstrated. To determine the extent of their contribution to T cell-mediated cytotoxicity, a range of effector cells from normal control or perforin-deficient mice were tested against a panel of target cells with various levels of Fas expression. All cytotoxicity observed was due to either of these mechanisms, and no third mechanism was detected. Thus, the perforin- and Fas-based mechanisms may account for all T cell-mediated cytotoxicity in short-term in vitro assays.

https://science.sciencemag.org/content/sci/265/5171/528.full.pdf

Fas and Perforin Pathways as Major Mechanisms of T Cell-Mediated Cytotoxicity

Evidence of an active linkage between the human genes that control lactate dehydrogenase B and peptidase B is presented. It is also concluded that there is no link between the genes for lactate dehydrogenase A and lactate dehydrogenase B.

Genetic Analysis with Man-Mouse Somatic Cell Hybrids: Linkage between Human Lactate Dehydrogenase B and Peptidase B Genes

Somatic hybridization between mouse cells and human leucocytes confirms the X-linkage of 8-azaguanine resistance in man and suggests that human lactate dehydrogenase A and B genes are not linked.

Genetic analysis with human--mouse somatic cell hybrids.

Cytolytic T lymphocyte (CTL) proliferation is dependent on T-cell growth factor (TCGF). However, fusion of mouse CTLs with T lymphoma cells yields two types of hybrids, cytolytic ones in which proliferation remains dependent on TCGF and others which express neither this dependence nor cytolytic function1. Clones of the first type can generate variants of the second type. TCGF-independent hybrids2 remain non-cytolytic when cultured in medium supplemented with supernatant of concanavalin A (Con A)-stimulated rat spleen cells (CS) which is our standard source of TCGF. Here we report that in TCGF-independent hybrids between a murine CTL line and a rat T lymphoma, high cytolytic activity can be induced by the addition of CS to the medium. The induction is reversible and seems to depend on a factor in CS which is different from TCGF and from immune interferon.

Hybrids between rat lymphoma and mouse t cells with inducible cytolytic activity.

SOMATIC cell hybridization has revealed important facts about gene activity, differentiation and expression of genetic information in cells1. Eliceiri and Green2 investigated the production of 28S ribosomal RNA in human–mouse hybrid cells and could detect only mouse ribosomal RNA. In a more recent study, however, Stanners, Eliceiri and Green3 showed that hamster–mouse hybrids synthesized both hamster and mouse ribosomal RNA.

Markus Nabholz

Papers

Genetic Analysis with Man-Mouse Somatic Cell Hybrids: Linkage between Human Lactate Dehydrogenase B and Peptidase B Genes

Genetic analysis with human--mouse somatic cell hybrids.

Hybrids between rat lymphoma and mouse t cells with inducible cytolytic activity.

Mitochondrial DNA of human-mouse cell hybrids.

Evolution of the Rh polymorphism: a model for the interaction of incompatibility, reproductive compensation, and heterozygote advantage.