Mary J. Chisholm

Bio: Mary J. Chisholm is an academic researcher from National Research Council. The author has contributed to research in topics: Fatty acid & Polyunsaturated fatty acid. The author has an hindex of 18, co-authored 45 publications receiving 846 citations.

Conjugated fatty acids of tragopogon and calendula seed oils

Abstract: The seed oil of Tragopogonporrifolius L. was found to contain about 4% of conjugated diene hydroxy fatty acids, identified as 9-hydroxy-10,12- and 13-hydroxy-9,11-octadecadienoic acids. On dehydrat...

A survey of the conjugated fatty acids of seed oils.

Abstract: Fatty acids with conjugated unsaturation occur in many seed oils. Thirty of these acids are reviewed with emphasis on their detection, isolation, and structure determination. Their distribution among plant families is shown, and a botanical source of each acid is given. Some reactions, derivatives, and methods of determining configuration are described. Current theories of their biosynthesis in the seed, involving oxygenated precursors, are summarized.

11-octadecenoic acid and other fatty acids of asclepias syriaca seed oil

Abstract: The seed oil of Asclepiassyriaca L., family Asclepidaceae, was examined by gas chromatography and distillation of the methyl esters. The fatty acids were found to include a considerable proportion ...

Fatty acid composition of some cucurbitaceae seed oils

Abstract: Seed oils of nine species of Cucurbitaceae were analyzed by gas chromatography and other techniques. The oil of Ecballiumelaterium yielded 22% of punicic acid; Cucumisdipsaceus and Cucurbitaficifol...

Kamlolenic acid and other conjugated fatty acids in certain seed oils

Abstract: The major fatty acid of the seed oil ofTrewia nudiflora is shown to be α-kamlolenic acid, not α-eleostearic acid as believed earlier. Other conjugated acids were found and identified in seed oils not previously studied, viz., α-eleostearic acid inParinari insularum andRicinodendron rautanenii; trans,8-trans,10-cis,12-octadecatrienoic acid inCalendula stellata.

Biology and biochemistry of glucosinolates

Abstract: Glucosinolates are sulfur-rich, anionic natural products that upon hydrolysis by endogenous thioglucosidases called myrosinases produce several different products (e.g., isothiocyanates, thiocyanates, and nitriles). The hydrolysis products have many different biological activities, e.g., as defense compounds and attractants. For humans these compounds function as cancer-preventing agents, biopesticides, and flavor compounds. Since the completion of the Arabidopsis genome, glucosinolate research has made significant progress, resulting in near-complete elucidation of the core biosynthetic pathway, identification of the first regulators of the pathway, metabolic engineering of specific glucosinolate profiles to study function, as well as identification of evolutionary links to related pathways. Although much has been learned in recent years, much more awaits discovery before we fully understand how and why plants synthesize glucosinolates. This may enable us to more fully exploit the potential of these compounds in agriculture and medicine.

Plant triacylglycerols as feedstocks for the production of biofuels

Abstract: Triacylglycerols produced by plants are one of the most energy-rich and abundant forms of reduced carbon available from nature. Given their chemical similarities, plant oils represent a logical substitute for conventional diesel, a non-renewable energy source. However, as plant oils are too viscous for use in modern diesel engines, they are converted to fatty acid esters. The resulting fuel is commonly referred to as biodiesel, and offers many advantages over conventional diesel. Chief among these is that biodiesel is derived from renewable sources. In addition, the production and subsequent consumption of biodiesel results in less greenhouse gas emission compared to conventional diesel. However, the widespread adoption of biodiesel faces a number of challenges. The biggest of these is a limited supply of biodiesel feedstocks. Thus, plant oil production needs to be greatly increased for biodiesel to replace a major proportion of the current and future fuel needs of the world. An increased understanding of how plants synthesize fatty acids and triacylglycerols will ultimately allow the development of novel energy crops. For example, knowledge of the regulation of oil synthesis has suggested ways to produce triacylglycerols in abundant non-seed tissues. Additionally, biodiesel has poor cold-temperature performance and low oxidative stability. Improving the fuel characteristics of biodiesel can be achieved by altering the fatty acid composition. In this regard, the generation of transgenic soybean lines with high oleic acid content represents one way in which plant biotechnology has already contributed to the improvement of biodiesel.

The nutritional significance, biosynthesis and bioavailability of glucosinolates in human foods

Abstract: The glucosinolates are a large group of sulphur-containing compounds which occur in all the economically important varieties of Brassica vegetable. Their common structure comprises a β-D-thioglucose group, a sulphonated oxime moiety and a variable side-chain derived from methionine, tryptophan or phenylalanine. When the plant tissue is damaged the glucosinolates are hydrolysed by the endogenous enzyme ‘myrosinase’ (thioglucoside glycohydrolase EC 3:2:3:1), to release a range of breakdown products including the bitter, biologically active isothiocyanates. Although these compounds exert antinutritional effects in animals there is also substantial evidence that they are the principal source of anticarcinogenic activity in Brassica vegetables, and this provides a strong motive for the manipulation of glucosinolate levels in vegetables for human consumption. This review provides an overview of the evidence for a beneficial role for glucosinolates in human health, and describes the current state of knowledge regarding the genetics and biosynthesis of glucosinolates, their chemical analysis, their behaviour during cooking and processing, and their bioavailability to humans. As the genetic basis of glucosinolate biosynthesis becomes more apparent, and tools for marker-assisted plant breeding become more available, the selective breeding of horticultural brassicas with different levels and types of glucosinolates, whether by conventional means or genetic manipulation, is becoming a practical possibility. However before this strategy becomes commercially viable, the health benefits of glucosinolates for human beings must be unequivocally established. © 2000 Society of Chemical Industry