Massimo Guardigli

Bio: Massimo Guardigli is an academic researcher from University of Bologna. The author has contributed to research in topics: Chemiluminescence & Lanthanide. The author has an hindex of 36, co-authored 112 publications receiving 5926 citations. Previous affiliations of Massimo Guardigli include Centre national de la recherche scientifique & Institut de Chimie des Substances Naturelles.

Engineering of highly luminescent lanthanide tags suitable for protein labeling and time-resolved luminescence imaging.

Abstract: The synthesis of a new ligand LH4 based on a glutamic acid skeleton bis-functionalized on its nitrogen atom by 6-methylene-6‘-carboxy-2,2‘-bipyridine chromophoric units is described. UV−vis spectrophotometric titrations revealed the formation of 1:1 M:L complexes with lanthanide(III) cations, and complexation of LH4 with equimolar amounts of hydrated LnCl3 salts (Ln = Eu, Gd, and Tb) gave water-soluble and stable complexes of the general formula [LnL(H2O)]Na, which were characterized by elemental analysis, IR, UV−vis absorption spectroscopy, 1H NMR (Ln = Eu), and mass spectrometry. The conditional stability constant for formation of the [EuL(H2O)]Na complex was determined by competitive complexation experiments to be log K = 16.5 ± 0.6 in 0.01 M TRIS/HCl buffer (pH = 7.0). In water solution, the [EuL(H2O)]Na and [TbL(H2O)]Na complexes were highly luminescent with quantum yields of 8% and 31%, respectively, despite the presence of ca. one water molecule in the first coordination sphere of the metal ions. A...

Analytical chemiluminescence and bioluminescence: latest achievements and new horizons

Abstract: Chemiluminescence (CL) and bioluminescence (BL) are the detection techniques of choice for the development of highly sensitive analytical methods, from immunoassays and nucleic acid hybridization assays to whole-cell biosensors. Nevertheless, basic and applied research on CL and BL aimed at further improving their analytical performance is still very active. This research covers diverse and complementary fields, including (among others) enhancing the light emission efficiency of CL systems, the use of nanomaterials to catalyze or enhance CL/BL reactions, the study of BL proteins to elucidate the color modulation mechanism, the discovery of new BL systems, the production of thermostable BL protein mutants with altered emission spectra, the development of BL imaging techniques to expand our understanding of living systems, and the implementation of CL/BL detection in miniaturized analytical devices. In the near future, we expect even greater diffusion of CL/BL-based analytical methods, especially in portable analytical devices intended for applications ranging from environmental analysis to companion diagnostics for personalized medicine.

A rapid multiplexed chemiluminescent immunoassay for the detection of Escherichia coli O157:H7, Yersinia enterocolitica, Salmonella typhimurium, and Listeria monocytogenes pathogen bacteria.

Abstract: A simple and rapid multiplexed sandwich chemiluminescent enzyme immunoassay has been developed for the simultaneous detection of Escherichia coli O157:H7, Yersinia enterocolitica, Salmonella typhimurium, and Listeria monocytogenes. To achieve the multiplexed detection of the four pathogens, a new polystyrene 96 well microtiter plate format has been designed, in which each main well contains four subwells in the bottom. The monoclonal antibodies specific for each bacteria were separately immobilized in each subwell. When the samples were added to the main wells, the bacteria able to specifically bind to the corresponding monoclonal antibody were captured in one of the four subwells. Subsequently, a mixture of peroxidase-labeled polyclonal antibodies against the four bacteria was added and the peroxidase activity of the bound polyclonal labeled antibodies in each well was measured by an enhanced luminol-based chemiluminescent cocktail using a low-light charge-coupled imaging device. The assay was simple and fast, and the limit of quantification was in the order of 104-105 CFU/mL for all bacterial species. The accuracy of the method, evaluated by comparison of the results with a conventional culturing methodology, was satisfactory, with recovery values ranging from 90 to 120%. This method can be used as a screening test to evaluate the presence of these pathogen bacteria in different foodstuffs.

Aggregation-Induced Emission: Together We Shine, United We Soar!

Abstract: Ju Mei,†,‡,∥ Nelson L. C. Leung,†,‡,∥ Ryan T. K. Kwok,†,‡ Jacky W. Y. Lam,†,‡ and Ben Zhong Tang*,†,‡,§ †HKUST-Shenzhen Research Institute, Hi-Tech Park, Nanshan, Shenzhen 518057, China ‡Department of Chemistry, HKUST Jockey Club Institute for Advanced Study, Institute of Molecular Functional Materials, Division of Biomedical Engineering, State Key Laboratory of Molecular Neuroscience, Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China Guangdong Innovative Research Team, SCUT-HKUST Joint Research Laboratory, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China

Luminescent Metal–Organic Frameworks

Abstract: Metal–organic frameworks (MOFs) display a wide range of luminescent behaviors resulting from the multifaceted nature of their structure. In this critical review we discuss the origins of MOF luminosity, which include the linker, the coordinated metal ions, antenna effects, excimer and exciplex formation, and guest molecules. The literature describing these effects is comprehensively surveyed, including a categorization of each report according to the type of luminescence observed. Finally, we discuss potential applications of luminescent MOFs. This review will be of interest to researchers and synthetic chemists attempting to design luminescent MOFs, and those engaged in the extension of MOFs to applications such as chemical, biological, and radiation detection, medical imaging, and electro-optical devices (141 references).