Showing papers by "Mawardi Rahmani published in 2012"
TL;DR: The results showed that PA possesses the characteristics of selectively inducing cell death of tumor cells as no inhibition was observed in non-tumorigenic cells even at 30 μg/ml.
106 citations
TL;DR: Pyranocycloartobiloxanthone A exhibited a strong free radical scavenger towards DPPH free radicals with IC50 value of 2 µg/mL with prominent discoloration observed in comparison with standard ascorbic acid, α-tocopherol and quercetin, and compound (1) displayed strong activity comparable with the standard kojic acid.
Abstract: One of the most promising plants in biological screening test results of thirteen Artocarpus species was Artocarpus obtusus FM Jarrett and detailed phytochemical investigation of powdered dried bark of the plant has led to the isolation and identification of three xanthones; pyranocycloartobiloxanthone A (1), dihydroartoindonesianin C (2) and pyranocycloartobiloxanthone B (3). These compounds were screened for antioxidant, antimicrobial and tyrosinase inhibitory activities. Pyranocycloartobiloxanthone A (1) exhibited a strong free radical scavenger towards DPPH free radicals with IC50 value of 2 µg/mL with prominent discoloration observed in comparison with standard ascorbic acid, α-tocopherol and quercetin, The compound also exhibited antibacterial activity against methicillin resistant Staphylococcus aureus (ATCC3359) and Bacillus subtilis (clinically isolated) with inhibition zone of 20 and 12 mm, respectively. However the other two xanthones were found to be inactive. For the tyrosinase inhibitory activity, again compound (1) displayed strong activity comparable with the standard kojic acid.
40 citations
TL;DR: Girinimbine, a carbazole alkaloid isolated from the stem bark of Murraya koenigii was tested for the in vitro anti-tumour promoting and antioxidant activities and was able to inhibit superoxide generation in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced differentiated premyelocytic HL-60 cells.
Abstract: Girinimbine, a carbazole alkaloid isolated from the stem bark of Murraya koenigii was tested for the in vitro anti-tumour promoting and antioxidant activities. Anti-tumour promoting activity was determined by assaying the capability of this compound to inhibit the expression of early antigen of Epstein-Barr virus (EA-EBV) in Raji cells that was induced by the tumour promoter, phorbol 12-myristate 13-acetate. The concentration of this compound that gave an inhibition rate at fifty percent was 6.0 µg/mL and was not cytotoxic to the cells. Immunoblotting analysis of the expression of EA-EBV showed that girinimbine was able to suppress restricted early antigen (EA-R). However, diffused early antigen (EA-D) was partially suppressed when used at 32.0 µg/mL. Girinimbine exhibited a very strong antioxidant activity as compared to a-tocopherol and was able to inhibit superoxide generation in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced differentiated premyelocytic HL-60 cells more than 95%, when treated with the compound at 5.3 and 26.3 µg/mL, respectively. However girinimbine failed to scavenge the stable diphenyl picryl hydrazyl (DPPH)-free radical.
38 citations
TL;DR: The continuing studies on secondary metabolites from the stem bark of Calophyllum soulattri has led to the isolation of another new diprenylated xanthone, phylattrin (1), in addition to five other xanthones and two common sterols.
Abstract: Our continuing studies on secondary metabolites from the stem bark of Calophyllum soulattri has led to the isolation of another new diprenylated xanthone, phylattrin (1), in addition to five other xanthones and two common sterols. The xanthones are soulattrin (2), caloxanthone C (3), macluraxanthone (4), brasixanthone B (5) and trapezifolixanthone (6) while the sterols are stigmasterol (7) and β-sitosterol (8). The structures of these compounds were determined on the basis of spectroscopic analyses such as 1D and 2D-NMR, HRESIMS, IR and UV. Compounds 1-7 exhibited moderate cytotoxic activities against SNU-1, HeLa, Hep G2, NCI-H23, K562, Raji, LS174T, IMR-32 and SK-MEL-28 cells.
22 citations
TL;DR: Pyranocycloartobiloxanthone A consistently showed strong cytotoxic activity against the three cell lines compared to the other two, and was not toxic towards normal cell lines human nontumorigenic breast cell line (MCF10A) and human peripheral blood mononuclear cells (PBMCs) with IC50 values of more than 30 μg/mL.
Abstract: An investigation of the chemical constituents in Artocarpus obtusus species led to the isolation of three new xanthones, pyranocycloartobiloxanthone A (1), dihydroartoindonesianin C (2), and pyranocycloartobiloxanthone B (3). The compounds were subjected to antiproliferative assay against human promyelocytic leukemia (HL60), human chronic myeloid leukemia (K562), and human estrogen receptor (ER+) positive breast cancer (MCF7) cell lines. Pyranocycloartobiloxanthone A (1) consistently showed strong cytotoxic activity against the three cell lines compared to the other two with IC50 values of 0.5, 2.0 and 5.0 μg/mL, respectively. Compound (1) was also observed to exert antiproliferative activity and apoptotic promoter towards HL60 and MCF7 cell lines at respective IC50 values. The compound (1) was not toxic towards normal cell lines human nontumorigenic breast cell line (MCF10A) and human peripheral blood mononuclear cells (PBMCs) with IC50 values of more than 30 μg/mL.
20 citations
TL;DR: Testing of the in vitro cytotoxic activities of all the isolated metabolites against a panel of human cancer cell lines Raji (lymphoma), SNU-1 (gastric carcinoma), K562 (erythroleukemia cells), HeLa (cervical cells), SK-MEL-28 and HeLa were strongly inhibited by stigmasterol (6) and beccamarin (2), indicating these secondary metabolites could be anti-cancer lead compounds in drug discovery
Abstract: An investigation on biologically active secondary metabolites from the stem bark of Mesua beccariana was carried out. A new cyclodione, mesuadione, along with several known constituents which are beccamarin, 2,5-dihydroxy-1,3,4-trimethoxy anthraquinone, 4-methoxy-1,3,5-trihydroxyanthraquinone, betulinic acid and stigmasterol were obtained from this ongoing research. Structures of these compounds were elucidated by extensive spectroscopic methods, including 1D and 2D-NMR, GC-MS, IR and UV techniques. Preliminary tests of the in vitro cytotoxic activities of all the isolated metabolites against a panel of human cancer cell lines Raji (lymphoma), SNU-1 (gastric carcinoma), K562 (erythroleukemia cells), LS-174T (colorectal adenocarcinoma), HeLa (cervical cells), SK-MEL-28 (malignant melanoma cells), NCI-H23 (lung adenocarcinoma), IMR-32 (neuroblastoma) and Hep-G2 (hepatocellular liver carcinoma) were carried out using an MTT assay. Mesuadione, beccamarin, betulinic acid and stigmasterol displayed strong inhibition of Raji cell proliferation, while the proliferation rate of SK-MEL-28 and HeLa were strongly inhibited by stigmasterol and beccamarin, indicating these secondary metabolites could be anti-cancer lead compounds in drug discovery.
20 citations
23 Jun 2012
TL;DR: Both extracts showed high bioactivity at all doses against C. cephalonica larvae and antifeedant action was increased with increasing plant extract concentrations, and could be used in IPM program for rice moth.
Abstract: Petroleum ether extract of black pepper (Piper nigrum) and physic nut (Jatropha curcas) were shown to have insecticidal efficacies against rice moth (Corcyra cephalonic) (Stainton). The C. cephalonica larvae (16 day old) were shown to have similarities susceptibility to petroleum ether extract of P. nigrum and J. curcas with LC50 values of 12.52 and 13.22 μL/mL, respectively. In a bioassay using no-choice tests, the parameters used to evaluate antifeedant activity were Relative Growth Rate (RGR); Relative Consumption Rate (RCR), efficiency on conversion of ingested food (ECI) and Feeding Deterrence Indices (FDI). Both extracts showed high bioactivity at all doses against C. cephalonica larvae and antifeedant action was increased with increasing plant extract concentrations. The petroleum ether extract of P. nigrum and J. curcas showed strong inhibition on egg hatchability and adult emergence of C. cephalonica at the lowest concentration. Based on the results of this study petroleum ether extracts of P. nigrum and J. curcas could be used in IPM program for rice moth.
11 citations
TL;DR: In this paper, the secondary metabolites from the roots of Mesua congestiflora were determined using spectroscopic methods which included 1D and 2D NMR, GC-MS, IR and UV techniques.
10 citations
01 Jan 2012
TL;DR: The investigation of the aerial parts of Haplophyllum laeviusculum (Rutaceae), a perennial herb native to Iran, afforded three alkaloids, skimmianine (1), lunamarine (2), ribalinidine (3) and two common sterols, γ-sitosterol and campesterol.
Abstract: The investigation of the aerial parts of Haplophyllum laeviusculum (Rutaceae), a perennial herb native to Iran, afforded three alkaloids, skimmianine (1), lunamarine (2), ribalinidine (3) and and two common sterols, γ-sitosterol and campesterol. The structures of compounds were identified by using spectroscopic methods by using UV, IR, NMR, MS spectra and also by comparison with previous works. There has been no detail phytochemical investigation report on the plant carried previously. This is the first report on the identification of skimmianine (1), lunamarine (2) from genus Haplophyllum.
8 citations
TL;DR: One new xanthone, mesuaferrin C (1), was isolated from the root bark of Mesua ferrea as discussed by the authors, and two other known xanthones which are macluraxanthone (2) and caloxanthone C (3), along with three triterpenoids, � -sitosterol (4), friedelin (5) and betulinic acid (6) were isolated as well.
Abstract: One new xanthone, mesuaferrin C (1), was isolated from the root bark of Mesua ferrea. Two other known xan- thones which are macluraxanthone (2) and caloxanthone C (3), along with three triterpenoids, � -sitosterol (4), friedelin (5) and betulinic acid (6) were isolated as well. The structures of these compounds were determined by 1D and 2D NMR and MS techniques.
7 citations
01 Jan 2012
TL;DR: The investigation of the aerial parts of Haplophyllum laeviusculum (Rutaceae), a perennial herb native to Iran, afforded three alkaloids, skimmianine (1), lunamarine (2), ribalinidine (3) and two common sterols, γ-sitosterol and campesterol.
Abstract: The investigation of the aerial parts of Haplophyllum laeviusculum (Rutaceae), a perennial herb native to Iran, afforded three alkaloids, skimmianine (1), lunamarine (2), ribalinidine (3) and and two common sterols, γ-sitosterol and campesterol. The structures of compounds were identified by using spectroscopic methods by using UV, IR, NMR, MS spectra and also by comparison with previous works. There has been no detail phytochemical investigation report on the plant carried previously. This is the first report on the identification of skimmianine (1), lunamarine (2) from genus Haplophyllum.