Melanie Koehler

TL;DR: It is demonstrated, both on model surfaces and on living cells, that the receptor binding domain (RBD) serves as the binding interface within the S-glycoprotein with the ACE2 receptor and the kinetic and thermodynamic properties of this binding pocket are extracted.

TL;DR: It is demonstrated that ligand selectivity is conferred by Reck, which mediates Wnt7-specific binding in a Frizzled-independent manner, and the Wnt decoding capacities of vertebrate cells and the functional diversification of Wnt family members are elucidated.

TL;DR: This review provides a detailed overview of the role of glycans in viral infection and highlights experimental approaches to study virus-glycan binding along with specific examples and highlights the development of the atomic force microscope to investigate interactions with glycans at the single-virion level directly on living mammalian cells.

TL;DR: Detailed procedures for probing the specific interactions of viruses with living cells are described; these procedures cover tip preparation, cell sample preparation, step-by-step FD-based AFM imaging and data analysis, and should be able to master the entire set of protocols in 1 month.

TL;DR: There is a direct link between increased RBD—ACE2 complex stability and the greater transmissibility observed for the variants of concern and insight into the impact of viral mutations on infection-induced immunity is provided.