M
Melvin F. Siliakus
Researcher at Wageningen University and Research Centre
Publications - 7
Citations - 473
Melvin F. Siliakus is an academic researcher from Wageningen University and Research Centre. The author has contributed to research in topics: Archaea & Membrane. The author has an hindex of 6, co-authored 6 publications receiving 318 citations. Previous affiliations of Melvin F. Siliakus include Children's Hospital Oakland Research Institute.
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Journal ArticleDOI
Adaptations of archaeal and bacterial membranes to variations in temperature, pH and pressure
TL;DR: It is demonstrated that the presence of membrane spanning ether-lipids and methyl branches shows a striking relationship with the growth boundaries of archaea and bacteria.
Journal ArticleDOI
Genetically manipulated phages with improved pH resistance for oral administration in veterinary medicine.
Franklin L. Nobrega,Ana Rita Costa,José de Freitas Santos,Melvin F. Siliakus,Jan W. M. van Lent,Servé W. M. Kengen,Joana Azeredo,Leon Kluskens +7 more
TL;DR: Phage engineering can be a good strategy to improve phage tolerance to GIT conditions, having promising application for oral administration in veterinary medicine, and is demonstrated to be simple and efficient.
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Converting Escherichia coli into an archaebacterium with a hybrid heterochiral membrane.
Antonella Caforio,Melvin F. Siliakus,Marten Exterkate,Samta Jain,Varsha R. Jumde,Ruben L. H. Andringa,Servé W. M. Kengen,Adriaan J. Minnaard,Arnold J. M. Driessen,John van der Oost +9 more
TL;DR: This finding challenges theories that assume that intrinsic instability of mixed membranes led to the “lipid divide” and the subsequent differentiation of bacteria and archaea and paves the way for future membrane engineering of industrial production organisms with improved robustness.
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Activity of the acyl-CoA synthetase ACSL6 isoforms: role of the fatty acid Gate-domains
TL;DR: Findings provide evidence that the diversity of these enzyme species could produce the variety of acyl-CoA synthetase activities that are necessary to generate and repair the hundreds of lipid species present in membranes.
Journal ArticleDOI
Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms
Nico J. Claassens,Melvin F. Siliakus,Sebastiaan K. Spaans,Sjoerd C.A. Creutzburg,Bart Nijsse,Peter J. Schaap,Tessa E. F. Quax,John van der Oost +7 more
TL;DR: It is shown that transcriptional fine-tuning plays a major role in improving the production of all tested proteins, and not a single algorithm performed consistently best for the membrane-integrated production of the 6 tested proteins.