Author
Michael A. Thorne
Bio: Michael A. Thorne is an academic researcher from Saint Louis University. The author has contributed to research in topics: Ether & Elution. The author has an hindex of 1, co-authored 1 publications receiving 62 citations.
Papers
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TL;DR: In this paper, the new phases OV-1 and OV17 were compared with QF-1 in the gas chromatographic analysis of 80 methyl 5β- and 5α-cholanoates and their complete trimethylsilyl (TMSi) ethers.
62 citations
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TL;DR: Observations are consistent with nearly all possible mechanisms of membrane assembly and may suggest that the phospholipid-rich membranes of embryonic muscle are gradually converted during development into calcium-transporting structures by stepwise insertion of the ATPase enzyme and other components of the transport system.
160 citations
TL;DR: It is indicated that considerable lithocholate is absorbed from the distal intestine in healthy subjects but efficient sulfation results in rapid fecal excretion, so that the total lithocholic pool remains small.
160 citations
121 citations
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01 Aug 1981121 citations
TL;DR: Strain HD-17, still unidentified, had both activities, but was unique in that it exclusively 7alpha-dehydroxylated cholic acid while biotransforming chenodeoxycholic acid, preferably though 7 alpha- dehydrogenation.
Abstract: Thirteen anaerobic bacteria capable of performing the 7 alpha-dehydroxylation of both cholic acid and chenodeoxycholic acid were isolated from human feces and also from sewage. Ten organisms from heat-treated samples were species of Clostridium identical or closely related to the Clostridium bifermentans-C. sordellii group and consisted of four strains elaborating 7 alpha-dehydroxylase alone and six strains capable of catalyzing both 7 alpha-dehydrogenation and 7 alpha-dehydroxylation. The remaining three organisms, recovered from fresh human feces, were gram-positive, nonflagellated, nonsporeforming, anaerobic rods and comprised two distinct species. Strain HD-17, still unidentified, had both activities, but was unique in that it exclusively 7 alpha-dehydroxylated cholic acid while biotransforming chenodeoxycholic acid, preferably though 7 alpha-dehydrogenation. Two unclassified strains, b-8 and c-25, metabolized both acids though 7 alpha-dehydroxylation and 7 alpha-dehydrogenation. Except for strains b-8 and c-25, all of th 7 alpha-dehydroxylating bacteria split the conjugated bile acid series, and hydrolases were detected in cell-free filtrates of early stationary-phase broth cultures.
109 citations