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Showing papers by "Michael Karin published in 1983"


Journal ArticleDOI
01 Jan 1983
TL;DR: A method for isolation of large, translationally active RNA species is presented and yields large mRNA precursors up to 10 kb and mRNA species which translate very well, however, small RNA species are recovered with a poor yield.
Abstract: A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5 m guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 m LiCl. Modifications are described for use with tissue culture cells, yeast, tissues, or isolated nuclei. The advantages of the procedure include speed, simplicity, avoidance of an ultracentrifugation, and its applicability to large numbers of small samples. The procedure yields large mRNA precursors up to 10 kb and mRNA species which translate very well. However, small (<300 nucleotides) RNA species are recovered with a poor yield.

1,348 citations


Patent
19 May 1983
TL;DR: Yeast metallothionein (copper chelatin) and DNA sequences having the gene encoding the polypeptide are provided in this paper, where the DNA sequences find use in producing coppers and in amplifying downstream flanking regions.
Abstract: Yeast metallothionein (copper chelatin) and DNA sequences having the gene encoding the polypeptide are provided. The DNA sequences find use in producing copper chelatin and in amplifying downstream flanking regions.

7 citations