Showing papers by "Michael Snyder published in 1985"
TL;DR: A colony color assay that measures chromosome stability is described and is used to study several parameters affecting the mitotic maintenance of yeast chromosomes, including ARS function, CEN function, and chromosome size.
422 citations
TL;DR: Gene disruption and Southern hybridization experiments show that the polymerase is encoded by an essential, single copy gene.
154 citations
01 Jan 1985
TL;DR: Methods for isolating eukaryotic genes by constructing and screening E. coli expression vector libraries with antibody probes using the bacteriophage expression vector λgt11 are described.
Abstract: Antibodies can be used as probes to isolate directly a particular gene of interest. A recombinant DNA library is first constructed in an Escherichia coli expression vector, and foreign antigens are expressed in E. coli from the DNA inserts. The antigens produced are transferred onto nitrocellulose filters and then probed with antibody to detect the desired recombinant. This approach is rapid, and is particularly useful for isolating genes that are expressed at a low level and therefore are difficult to isolate using techniques employing nucleic acid probes (see Ref. 1) or by hybrid/selection methods.2 This chapter describes methods for isolating eukaryotic genes by constructing and screening E. coli expression vector libraries with antibody probes using the bacteriophage expression vector λgt11.3,4
18 citations