Michaela Fellner

TL;DR: The generation and validation of a genome-wide library of Drosophila melanogaster RNAi transgenes, enabling the conditional inactivation of gene function in specific tissues of the intact organism and opening up the prospect of systematically analysing gene functions in any tissue and at any stage of the Drosophile lifespan.

TL;DR: A role in muscle is identified for 2,785 genes, many of which are phylogenetically conserved, including genes implicated in mammalian sarcomere organization and human muscle diseases.

TL;DR: The findings reveal the histone chaperone CAF-1 to be a novel regulator of somatic cell identity during transcription-factor-induced cell-fate transitions and provide a potential strategy to modulate cellular plasticity in a regenerative setting.

TL;DR: A scalable strategy toward glue degrader discovery is described that is based on chemical screening in hyponeddylated cells coupled to a multi-omics target deconvolution campaign to identify compounds that induce ubiquitination and degradation of cyclin K by prompting an interaction of CDK12–cyclin K with a CRL4B ligase complex.

TL;DR: Using optimized screening workflows, the Vienna Bioactivity CRISPR (VBC) scoring system provides an improved selection for sgRNAs that generate loss-of-function alleles, and outperforms previous prediction tools and enables the selection of sg RNAs that effectively produce loss- of- function alleles.