M
Michele A. Cleary
Researcher at Merck & Co.
Publications - 58
Citations - 11625
Michele A. Cleary is an academic researcher from Merck & Co.. The author has contributed to research in topics: Gene silencing & microRNA. The author has an hindex of 36, co-authored 58 publications receiving 11107 citations. Previous affiliations of Michele A. Cleary include Stony Brook University & Cold Spring Harbor Laboratory.
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Journal ArticleDOI
A microRNA component of the p53 tumour suppressor network
Lin He,Xingyue He,Xingyue He,Lee P. Lim,Elisa de Stanchina,Elisa de Stanchina,Zhenyu Xuan,Yu Liang,Wen Xue,Lars Zender,Jill F. Magnus,Dana Ridzon,Aimee L. Jackson,Peter S. Linsley,Caifu Chen,Scott W. Lowe,Michele A. Cleary,Gregory J. Hannon +17 more
TL;DR: A family of miRNAs, miR-34a–c, whose expression reflected p53 status is described, whose encoded genes are direct transcriptional targets of p53, whose induction by DNA damage and oncogenic stress depends on p53 both in vitro and in vivo.
Journal ArticleDOI
Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity.
Aimee L. Jackson,Julja Burchard,Janell M. Schelter,B. Nelson Chau,Michele A. Cleary,Lee Lim,Peter S. Linsley +6 more
TL;DR: In all cases, off-target transcript silencing was accompanied by loss of the corresponding protein and occurred with dependence on siRNA concentration similar to that of silencing of the target transcript.
Journal ArticleDOI
A resource for large-scale RNA-interference-based screens in mammals
Patrick J. Paddison,Jose M. Silva,Douglas S. Conklin,Douglas S. Conklin,Mike Schlabach,Mike Schlabach,Mamie Li,Shola Aruleba,Vivekanand Balija,A. O'Shaughnessy,Lidia Gnoj,Kim Scobie,Kenneth Chang,Thomas F. Westbrook,Thomas F. Westbrook,Michele A. Cleary,Ravi Sachidanandam,W. Richard McCombie,Stephen J. Elledge,Stephen J. Elledge,Gregory J. Hannon +20 more
TL;DR: The construction and application of a shRNA expression library targeting 9,610 human and 5,563 mouse genes is reported, which suggests that the large-scale RNAi library can be used in specific, genetic applications in mammals, and will become a valuable resource for gene analysis and discovery.
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Second-generation shRNA libraries covering the mouse and human genomes.
Jose M. Silva,Mamie Z. Li,Kenneth Chang,Wei Ge,Michael C. Golding,Richard J. Rickles,Despina Siolas,Guang Hu,Patrick J. Paddison,Michael R. Schlabach,Nihar U. Sheth,Jeff Bradshaw,Julia Burchard,Amit A. Kulkarni,Guy Cavet,Ravi Sachidanandam,W. Richard McCombie,Michele A. Cleary,Stephen J. Elledge,Gregory J. Hannon +19 more
TL;DR: Large-scale-arrayed, sequence-verified libraries comprising more than 140,000 second-generation short hairpin RNA expression plasmids, covering a substantial fraction of all predicted genes in the human and mouse genomes are generated.
Journal ArticleDOI
Transcripts targeted by the microRNA-16 family cooperatively regulate cell cycle progression
Peter S. Linsley,Janell M. Schelter,Julja Burchard,Miho Kibukawa,Melissa M. Martin,Steven R. Bartz,Jason M. Johnson,Jordan M. Cummins,Christopher K. Raymond,Hongyue Dai,Nelson Chau,Michele A. Cleary,Aimee L. Jackson,Michael Carleton,Lee Lim +14 more
TL;DR: It is demonstrated that a family of miRNAs sharing sequence identity with miRNA-16 (miR-16) negatively regulates cellular growth and cell cycle progression and coordinately regulates targets that may act in concert to controlcell cycle progression.