Michèle Bernier-Cardou

TL;DR: Improvements and simplifications of SE protocols in Pinus pinaster (Ait), a species of economic importance in the regions of Western Europe, are described including high initiation frequencies in eight control pollinated seed families, relatively high somatic embryo maturation yield when cells were coated with particles of activated charcoal and a rapid production of plants directly in a shade house.

TL;DR: It is concluded that the embryogenic tissue was exposed to varying amounts of water at the onset of and during the culture period, and that the amount of water in the culture environment in turn influenced the maturation response.

TL;DR: The abundance of competing species has considerably reduced site production for a period of 20 years and will probably continue to do so for 20 to 30 more years, meaning it is unlikely that the harvested areas will naturally evolve toward the original climax balsam fir-yellow birch forest type in the foreseeable future.

TL;DR: An improved method for accelerated production of large quantities of Scots pine for clonal tests is described and mature somatic embryos accumulated amounts of storage proteins that were similar to the amounts in mature zygotic embryos.

TL;DR: Increasing sucrose concentration or substituting part of the sucrose with mannitol or sorbitol had variable effects on somatic embryo maturation depending on the embryogenic line, and complex combinations of amino acids had little effect on mature embryo production of most tested embryogenic lines.