Bio: Miho Ishii-Teshima is an academic researcher from Toyohashi University of Technology. The author has contributed to research in topics: Titanium oxide & Perforation (oil well). The author has an hindex of 2, co-authored 2 publications receiving 8 citations.
TL;DR: In this article, a nanosecond pulse laser-assisted photoporation using titanium-oxide nanotubes (TNT) for highly efficient intracellular delivery has been established.
Abstract: In the present study, a newly developed nanosecond pulse laser-assisted photoporation using titanium-oxide nanotubes (TNT) for highly efficient intracellular delivery has been established. The proof of concept for the possibilities of intracellular delivery after irradiation of nanosecond pulse laser on TNT has been validated. TNT on titanium sheets using the electrochemical anodization technique at different voltage and time has been developed. The extensive X-ray photoelectron spectroscopy (XPS) study confirms the presence of different titanium oxide species such as TiO2, TixOy (TiO/Ti2O3/Ti3O5) having different concentrations in TNT formed by different anodization voltage and time along with a minor quantity of Ti metal (Ti0). Formation of sub-oxides results in oxygen defects in TNT. It has also been evidenced from XPS that the anodization voltage and time can change the concentration of oxygen defects on the nanotubes. Due to the formation of oxygen defects, nanotubes have the quasi-metallic and metallic properties. These properties of the nanotubes may facilitate the intracellular delivery by various mechanisms after irradiation of nanosecond pulse laser. Using this technique, we successfully have delivered Propidium iodide (PI) and dextran into HeLa cells (HeLa- human cervical cancer cells) with high transfection efficiency and cell viability on nanotubes formed at 15 V/2 h.
TL;DR: It is successfully demonstrated that the intracellular TERS imaging has the potential to visualize distinctly different features in Raman spectra between the nucleus and the cytoplasm of a single living cell and to analyze the dynamic behavior of biomolecules inside a living cell.
Abstract: Atomic force microscopy (AFM) is an effective platform for in vitro manipulation and analysis of living cells in medical and biological sciences. To introduce additional new features and functionalities into a conventional AFM system, we investigated the photocatalytic nanofabrication and intracellular Raman imaging of living cells by employing functionalized AFM probes. Herein, we investigated the effect of indentation speed on the cell membrane perforation of living HeLa cells based on highly localized photochemical oxidation with a catalytic titanium dioxide (TiO2)-functionalized AFM probe. On the basis of force-distance curves obtained during the indentation process, the probability of cell membrane perforation, penetration force, and cell viability was determined quantitatively. Moreover, we explored the possibility of intracellular tip-enhanced Raman spectroscopy (TERS) imaging of molecular dynamics in living cells via an AFM probe functionalized with silver nanoparticles in a homemade Raman system integrated with an inverted microscope. We successfully demonstrated that the intracellular TERS imaging has the potential to visualize distinctly different features in Raman spectra between the nucleus and the cytoplasm of a single living cell and to analyze the dynamic behavior of biomolecules inside a living cell.
TL;DR: Advances in temporal resolution with the use of line scanning and height spectroscopy techniques show how high-speed atomic force microscopy can measure millisecond to microsecond dynamics, pushing this method beyond current spatial and temporal limits offered by less direct techniques.
Abstract: Recent advances in high-speed atomic force microscopy (HS-AFM) have made it possible to study the conformational dynamics of single unlabeled transmembrane channels and transporters. Improving environmental control with the integration of a non-disturbing buffer exchange system, which in turn allows the gradual change of conditions during HS-AFM operation, has provided a breakthrough toward the performance of structural titration experiments. Further advancements in temporal resolution with the use of line scanning and height spectroscopy techniques show how high-speed atomic force microscopy can measure millisecond to microsecond dynamics, pushing this method beyond current spatial and temporal limits offered by less direct techniques.
TL;DR: In this paper, the authors present a review of Raman spectroscopy-based methods for single-cell analysis, focusing on Raman imaging of single cells to study the intracellular distribution of different components.
Abstract: The conventional microbial cell analyses are mostly population-averaged methods that conceal the characteristics of single-cell in the community. Single-cell analysis can provide information on the functional and structural variation of each cell, resulting in the elimination of long and tedious microbial cultivation techniques. Raman spectroscopy is a label-free, noninvasive, and in-vivo method ideal for single-cell measurement to obtain spatially resolved chemical information. In the current review, recent developments in Raman spectroscopic techniques for microbial characterization at the single-cell level are presented, focusing on Raman imaging of single cells to study the intracellular distribution of different components. The review also discusses the limitation and challenges of each technique and put forward some future outlook for improving Raman spectroscopy-based techniques for single-cell analysis. Raman spectroscopic methods at the single-cell level have potential in precision measurements, metabolic analysis, antibiotic susceptibility testing, resuscitation capability, and correlating phenotypic information to genomics for cells, the integration of Raman spectroscopy with other techniques such as microfluidics, stable isotope probing (SIP), and atomic force microscope can further improve the resolution and provide extensive information. Future focuses should be given to advance algorithms for data analysis, standardized reference libraries, and automated cell isolation techniques in future.
TL;DR: A brief analysis of existing studies on the application of Raman spectroscopy for investigation of biological, medical, analytical, photosynthetic, and algal research, to understand how the technique can be used for lipids, carotenoids, and cellular research.
Abstract: Nowadays, there is an interest in biomedical and nanobiotechnological studies, such as studies on carotenoids as antioxidants and studies on molecular markers for cardiovascular, endocrine, and oncological diseases. Moreover, interest in industrial production of microalgal biomass for biofuels and bioproducts has stimulated studies on microalgal physiology and mechanisms of synthesis and accumulation of valuable biomolecules in algal cells. Biomolecules such as neutral lipids and carotenoids are being actively explored by the biotechnology community. Raman spectroscopy (RS) has become an important tool for researchers to understand biological processes at the cellular level in medicine and biotechnology. This review provides a brief analysis of existing studies on the application of RS for investigation of biological, medical, analytical, photosynthetic, and algal research, particularly to understand how the technique can be used for lipids, carotenoids, and cellular research. First, the review article shows the main applications of the modified Raman spectroscopy in medicine and biotechnology. Research works in the field of medicine and biotechnology are analysed in terms of showing the common connections of some studies as caretenoids and lipids. Second, this article summarises some of the recent advances in Raman microspectroscopy applications in areas related to microalgal detection. Strategies based on Raman spectroscopy provide potential for biochemical-composition analysis and imaging of living microalgal cells, in situ and in vivo. Finally, current approaches used in the papers presented show the advantages, perspectives, and other essential specifics of the method applied to plants and other species/objects.
••11 Jan 2021
TL;DR: A solution for complex system by coupling AFM with finite element simulations to retrieve more quantitative information when heterogeneity and convolution play important roles is proposed.
Abstract: Nanomechanics of cytoskeleton is deeply involved in physiology and regulation of cell behavior. Atomic Force Microscopy has been extensively used for quantitative characterization with high-spatial...
TL;DR: In this article , a comprehensive review focuses on the appropriate phase and composition for new Ti alloys intended for use as biomedical implants, emphasizing both fabrication and surface modification methods. But, major characteristics highlighted the importance of elastic modulus and the use of non-toxic metal elements to improve biocompatibility.
Abstract: Titanium and its alloys have long been used as implant materials due to their outstanding mechanical properties and apparent biocompatibility. Despite this, the search for better alloys has continued to be active by researchers and industries alike, as there are still pressing issues that require attention. These include (1) a large mismatch in the elastic modulus of the implant material, which causes a stress shielding problem; (2) the release of harmful ions from Ti alloys after long-term use; (3) a low bioactivity of the Ti alloy surface, which prolongs the healing process. More research has been directed toward finding new generation Ti alloys composed of more biocompatible phases and modifying the surface of Ti alloys from naturally bio-inert to bioactive in order to circumvent the problems. This review examines recent work reported on the fabrication of Ti alloys, and based on the survey, major characteristics highlighted the importance of elastic modulus and the use of non-toxic metal elements to improve biocompatibility. In terms of surface modification of Ti alloys, numerous studies have found that a nano-scaled surface oxide layer grown on the surface is always beneficial to improving the bioactivity of Ti alloys for rapid recovery after implantation. This comprehensive review focuses on the appropriate phase and composition for new Ti alloys intended for use as biomedical implants, emphasizing both fabrication and surface modification methods.