Author
Ming-Hua Yang
Bio: Ming-Hua Yang is an academic researcher. The author has contributed to research in topics: Medicine & Procalcitonin. The author has an hindex of 1, co-authored 1 publications receiving 3197 citations.
Papers
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TL;DR: In this article, two complementary colorimetric methods, aluminum chloride method and 2,4-dini trophenylhydrazine method, were used to determine the real content of total flavonoids in propolis.
3,899 citations
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TL;DR: The mechanisms of action of various cytokines are summarized and a therapeutic overview of the current targeted therapies for HLH is provided to help for early treatment to reduce the mortality of HLH.
4 citations
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TL;DR: The mNGS technique has a shorter turnaround time and a higher sensitivity for pathogen detection and can provide evidence for the pathogenic diagnosis of children with agranulocytosis and fever.
Abstract: OBJECTIVES
To study the application value of metagenomic next-generation sequencing (mNGS) for pathogen detection in childhood agranulocytosis with fever.
METHODS
A retrospective analysis was performed on the mNGS results of pathogen detection of 116 children with agranulocytosis with fever who were treated from January 2020 to December 2021. Among these children, 38 children with negative mNGS results were enrolled as the negative group, and 78 children with positive results were divided into a bacteria group (n=22), a fungal group (n=23), and a viral group (n=31). Clinical data were compared between groups.
RESULTS
For the 116 children with agranulocytosis and fever, the median age was 8 years at diagnosis, the median turnaround time of mNGS results was 2 days, and the positive rate of mNGS testing was 67.2% (78/116). Compared with the negative group, the bacterial group had a higher procalcitonin level (P<0.05), the fungal group had higher level of C-reactive protein and positive rate of (1,3)-β-D glucan test/galactomannan test (P<0.05), and the fungal group had a longer duration of fever (P<0.05). Among the 22 positive microbial culture specimens, 9 (41%) were consistent with the mNGS results. Among the 17 positive blood culture specimens, 8 (47%) were consistent with the mNGS results. Treatment was adjusted for 28 children (36%) with the mNGS results, among whom 26 were cured and discharged.
CONCLUSIONS
The mNGS technique has a shorter turnaround time and a higher sensitivity for pathogen detection and can provide evidence for the pathogenic diagnosis of children with agranulocytosis and fever.
Cited by
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TL;DR: In this paper, several honey samples (27) from Burkina Faso were analyzed to determine their total phenolic, flavonoid and proline contents as well as their radical scavenging activity.
1,850 citations
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TL;DR: Results indicate that L. aromatica can be used in dietary applications with a potential to reduce oxidative stress.
1,176 citations
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TL;DR: In this article, the relative antioxidant activity in selected Iranian medicinal plant species' extracts was determined spectrophotometrically, and the highest radical scavenging effect was observed in Mellilotus officinalis with IC50 = 0.018 mg ml −1.
Abstract: In present study, we carried out a systematic record of the relative antioxidant activity in selected Iranian medicinal plant species' extracts. The total phenol varied from 24.1 ± 1 to 289.5 ± 5 mg g -1 in the extracts. Flavonoid contents were between 25.15 ± 0.8 and 78.3 ± 4.5 mg g-1. 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging effect of the extracts was determined spectrophotometrically. The highest radical scavenging effect was observed in Mellilotus officinalis with IC50 = 0.018 mg ml –1. The potency of radical scavenging effect of M. officinalis extract was about 4 times greater than synthetic antioxidant butylated hydroxy toluene (BHT). The greater amount of phenolic compounds leads to morepotent radical scavenging effect as shown by M. officinalis extract
1,029 citations
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TL;DR: This review intends to offer a critical evaluation of existing antioxidant assays applied to phenolics, and reports the development of a simple and low-cost antioxidant capacity assay for dietary polyphenols, vitamins C and E, and human serum antioxidants, which is named the CUPRAC (cupric ion reducing antioxidant capacity) method.
Abstract: It would be desirable to establish and standardize methods that can measure the total antioxidant capacity level directly from vegetable extracts containing phenolics. Antioxidant capacity assays may be broadly classified as electron transfer (ET)- and hydrogen atom transfer (HAT)-based assays. The majority of HAT assays are kinetics-based, and involve a competitive reaction scheme in which antioxidant and substrate compete for peroxyl radicals thermally generated through the decomposition of azo compounds. ET-based assays measure the capacity of an antioxidant in the reduction of an oxidant, which changes colour when reduced. ET assays include the ABTS/TEAC, CUPRAC, DPPH, Folin-Ciocalteu and FRAP methods, each using different chromogenic redox reagents with different standard potentials. This review intends to offer a critical evaluation of existing antioxidant assays applied to phenolics, and reports the development by our research group of a simple and low-cost antioxidant capacity assay for dietary polyphenols, vitamins C and E, and human serum antioxidants, utilizing the copper(II)-neocuproine reagent as the chromogenic oxidizing agent, which we haved named the CUPRAC (cupric ion reducing antioxidant capacity) method. This method offers distinct advantages over other ET-based assays, namely the selection of working pH at physiological pH (as opposed to the Folin and FRAP methods, which work at alkaline and acidic pHs, respectively), applicability to both hydrophilic and lipophilic antioxidants (unlike Folin and DPPH), completion of the redox reactions for most common flavonoids (unlike FRAP), selective oxidation of antioxidant compounds without affecting sugars and citric acid commonly contained in foodstuffs and the capability to assay -SH bearing antioxidants (unlike FRAP). Other similar ET-based antioxidant assays that we have developed or modified for phenolics are the Fe(III)- and Ce(IV)-reducing capacity methods.
921 citations
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TL;DR: The highest total phenolic content was observed in mulberry (1515.9 ± 5.7 mg gallic acid equivalents (GAE)/100 g fresh matter (FM) among four selected fruit species as discussed by the authors.
773 citations