Author
Mona E. Fewster
Bio: Mona E. Fewster is an academic researcher from University of California, Los Angeles. The author has contributed to research in topics: Cerebroside & Measles. The author has an hindex of 10, co-authored 13 publications receiving 705 citations.
Topics: Cerebroside, Measles, Myelin, Multiple sclerosis, Choline
Papers
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TL;DR: A copper acetate spray reagent has been used for the quantitative densitometric thin-layer chromatography of glycolipids and phospholipids from human CNS myelin and the precision ranges between zero and ± 4.5%.
396 citations
TL;DR: Glial cells were isolated from bovine white matter by differential centrifugation with‘Ficoll’ and their lipid composition was analysed, finding that EGP contained the largest proportion of aldehydes and SGP contained 12 per cent, and no gangliosides were present in the filial cell preparations.
Abstract: — —Glial cells were isolated from bovine white matter by differential centrifugation with‘Ficoll’and their lipid composition was analysed. The preparations contained 20.8 per cent lipid and 792 per cent protein. The major lipid components were cholesterol, ethanolamine glycerophosphatides (EGP), cerebroside and serine glycerophosphatides (SGP). Sphingomyelin, cerebroside sulphate and inositol glycerophosphatide were present in lower proportions. EGP contained the largest proportion of aldehydes (17 per cent) and SGP contained 12 per cent. Choline glycerophosphatides contained only a trace of aldehyde. No gangliosides were present in the filial cell preparations.
62 citations
TL;DR: The extent of integrity of the isolated glial cells should enable more extended studies to be conducted using larger quantities ofglial cells of comparable purity than have hitherto been available.
50 citations
TL;DR: The quantitative isolation of oligodendroglia from bovine white matter was accomplished by the modifications of an earlier procedure and DNA, RNA and protein contents of the cells were determined.
35 citations
TL;DR: Glial cells were isolated from bovine white matter by differential centrifugation and long‐chain fatty acids were detected in sphingomyelin, cerebroside and cerebro side sulphate; this indicates that chain‐elongation beyond C18 occurs in the glial cells.
Abstract: — Glial cells were isolated from bovine white matter by differential centrifugation. The fatty aldehyde and fatty acid compositions of ethanolamine glycerophosphatides (EGP), serine glycerophosphatides (SGP) and choline glycerophosphatides (CGP) were determined by gas-liquid chromatography. The fatty acid compositions of the sphingo-lipids including sphingomyelin, cerebroside and cerebroside sulphate, and of minor lipid components including cholesterol esters and triglycerides, were also determined by gas-liquid chromatography. The relative proportions correlated closely with the results obtained by O'Brien and Sampson (1965b) for adult human brain. The fatty aldehyde compositions of the glycerophosphatides were more closely related to the corresponding fatty acid compositions of the plasma membrane than of the mitochondria. Long-chain fatty acids (19–26 carbon atoms) were detected in sphingomyelin, cerebroside and cerebroside sulphate; this indicates that chain-elongation beyond C18 occurs in the glial cells.
34 citations
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TL;DR: An improved method for extracting the lipids from tissues consists of the use of hexane:isopropanol, followed by a wash of the extract with aqueous sodium sulfate to remove nonlipid contaminants.
2,400 citations
TL;DR: The mobile phases described permit separation of the six major phospholipids of amniotic fluid in one dimension with either conventional or high performance thin layer chromatography.
Abstract: The mobile phases described permit separation of the six major phospholipids of amniotic fluid in one dimension with either conventional or high performance thin layer chromatography. An example of this separation with an extract of amniotic fluid is given.
470 citations
TL;DR: Crassostrea gigas spat were fed diets of Pavlova lutheri, Dunaliella tertiolecta or Tetraselmis suecica as discussed by the authors.
Abstract: Crassostrea gigas spat were fed diets of Pavlova lutheri, Dunaliella tertiolecta or Tetraselmis suecica . The D. tertiolecta diet contained no fatty acid longer or more unsaturated than linolenic acid (18:3ω3) and the T. suecica diet was deficient in decosahexaenoic acid (22:6ω3). The algal diets were supplemented with micro-encapsulated triolein, oyster lipid extract or 22:6ω3. Spat were also starved or fed carbohydrate diets.
451 citations
TL;DR: The high-performance thin-layer chromatography method can be applied to lipids extracted from marine organisms including the complex polar lipids derived from phytoplankton and fish neural tissue.
397 citations
TL;DR: A copper acetate spray reagent has been used for the quantitative densitometric thin-layer chromatography of glycolipids and phospholipids from human CNS myelin and the precision ranges between zero and ± 4.5%.
396 citations