Author
Morfin R
Bio: Morfin R is an academic researcher from Albert Einstein College of Medicine. The author has contributed to research in topics: Heparin & Protamine. The author has an hindex of 2, co-authored 3 publications receiving 2409 citations.
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TL;DR: With a single preparative ultracentrifugation, immunologically pure high density lipoproteins can be isolated from large volumes of serum.
2,449 citations
TL;DR: In this article, a precipitation fractionnee des lipoproteines seriques est de ce fait possible ; dans un premier temps on insolubilise les β-lipoproteine, and, in un deuxieme, les α-lipiproteine.
42 citations
Journal Article•
2 citations
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TL;DR: With a single preparative ultracentrifugation, immunologically pure high density lipoproteins can be isolated from large volumes of serum.
2,449 citations
TL;DR: A simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low- density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+.
Abstract: We describe a simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low-density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+. Values so obtained correlate well with values obtained with the heparin-Mn2+ precipitation technique (r = 0.95, CV less than 5% in 66% of the subjects studied and between 5 and 10% in the remaining ones) or by ultracentrifugal separation (r = 0.82, CV less than 5% in 80% of the subjects studied and between 5 and 10% in the remaining ones). Our precipitation technique is more appropriate for routine clinical laboratory use.
1,802 citations
TL;DR: Determination of the extent of the unprecipitated apoB-associated lipoproteins by sensitive radioimmunoassay and of the amount of precipitated high density lipoprotein by radial immunodiffusion assay of apolipoproteins A-I and A-II at various heparin and Mn(2+) concentrations indicated that the usual Heparin level was adequate.
1,297 citations
TL;DR: The relationship of future clinical coronary heart-disease to the plasma-high-density-lipoprotein (H.H.D.L.)-cholesterol concentration has been examined in a 2-year case-control follow-up study of 6595 men aged 20-49 years living in the municipality of Tromsø, Norway and support the proposal that a low H.d.L. cholesterol concentration is important in accelerating the progression of coronary atherosclerosis.
1,180 citations
TL;DR: In this article, the relation between the fatty acid composition of skeletal-muscle phospholipids and insulin sensitivity was determined in two groups of subjects, including 27 patients undergoing coronary artery surgery and 13 normal men.
Abstract: Background Insulin resistance and hyperinsulinemia are features of obesity, non-insulin-dependent diabetes mellitus, and other disorders. Skeletal muscle is a major site of insulin action, and insulin sensitivity may be related to the fatty-acid composition of the phospholipids within the muscle membranes involved in the action of insulin. Methods We determined the relation between the fatty-acid composition of skeletal-muscle phospholipids and insulin sensitivity in two groups of subjects. In one study, we obtained samples of the rectus abdominis muscle from 27 patients undergoing coronary artery surgery; fasting serum insulin levels provided an index of insulin sensitivity. In the second study, a biopsy of the vastus lateralis muscle was performed in 13 normal men, and insulin sensitivity was assessed by euglycemic-clamp studies. Results In the patients undergoing surgery, the fasting serum insulin concentration (a measure of insulin resistance) was negatively correlated with the percentage of individua...
834 citations