scispace - formally typeset
Search or ask a question
Author

Muhammad Sajjad Ansari

Bio: Muhammad Sajjad Ansari is an academic researcher from University of Sargodha. The author has contributed to research in topics: Extender & Sperm motility. The author has an hindex of 17, co-authored 65 publications receiving 863 citations. Previous affiliations of Muhammad Sajjad Ansari include University of Gujrat & Pir Mehr Ali Shah Arid Agriculture University.
Topics: Extender, Sperm motility, Sperm, Semen, Semen quality


Papers
More filters
Journal ArticleDOI
TL;DR: Duck egg yolk compared to other avian yolks in extender improves the frozen-thawed quality of buffalo bull spermatozoa.

101 citations

Journal ArticleDOI
TL;DR: It is concluded that commercially available Bioxcell may be used for the cryopreservation of buffalo semen with an equal efficiency to tris-citric egg yolk extender.

66 citations

Journal ArticleDOI
TL;DR: Tris-citric acid extender supplemented with glutathione improved the freezability of buffalo bull spermatozoa in a dose dependant manner and the addition of 2.0 mM GSH to the extender enhanced the in vivo fertility of buffalo (Bubalus bubalis) bull spermzoa.

51 citations

Journal ArticleDOI
TL;DR: In this article, the effect of Antarctic fish antifreeze glycoproteins (AFGP) size 1-5 (34-10.5kDa), 7-8 (3.2 and 2.4kDa) in extender on buffalo bull sperm at cooling (4°C) and at post thawing was evaluated.

47 citations

Journal ArticleDOI
TL;DR: In conclusion addition of AFP III in the extender at 0.1 μg/mL improved the progressive motility and plasma membrane integrity of cryopreserved buffalo bull semen.

44 citations


Cited by
More filters
Journal ArticleDOI
TL;DR: Improved cryopreservation success will be an essential step in many future areas such as regenerative medicine, seed banking, or stem cell technology, where better and safer CPAs will be key requirements.

311 citations

Journal ArticleDOI
TL;DR: The applications of IBPs in the food industry, in cryopreservation, and in other technologies are vast, and some possibilities are charted out.
Abstract: Ice-binding proteins (IBPs) are a diverse class of proteins that assist organism survival in the presence of ice in cold climates. They have different origins in many organisms, including bacteria, fungi, algae, diatoms, plants, insects, and fish. This review covers the gamut of IBP structures and functions and the common features they use to bind ice. We discuss mechanisms by which IBPs adsorb to ice and interfere with its growth, evidence for their irreversible association with ice, and methods for enhancing the activity of IBPs. The applications of IBPs in the food industry, in cryopreservation, and in other technologies are vast, and we chart out some possibilities.

254 citations

Journal ArticleDOI
TL;DR: Current freezing techniques and novel strategies that have been developed for sperm protection against cryo-damage are discussed, as well as evaluating the probable effects of sperm freezing on offspring health.
Abstract: The cryopreservation of spermatozoa was introduced in the 1960s as a route to fertility preservation. Despite the extensive progress that has been made in this field, the biological and biochemical mechanisms involved in cryopreservation have not been thoroughly elucidated to date. Various factors during the freezing process, including sudden temperature changes, ice formation and osmotic stress, have been proposed as reasons for poor sperm quality post-thaw. Little is known regarding the new aspects of sperm cryobiology, such as epigenetic and proteomic modulation of sperm and trans-generational effects of sperm freezing. This article reviews recent reports on molecular and cellular modifications of spermatozoa during cryopreservation in order to collate the existing understanding in this field. The aim is to discuss current freezing techniques and novel strategies that have been developed for sperm protection against cryo-damage, as well as evaluating the probable effects of sperm freezing on offspring health.

223 citations

Journal ArticleDOI
TL;DR: Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state and the major factors that may influence the post-thaw viability and fertility of buffalo spermatozoa are examined in detail.
Abstract: Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state is discussed in this article. Fertility rate in buffalo following artificial insemination with frozen-thawed semen is reviewed. To better understand the freezability of bubaline spermatozoa, the available data on biochemical components and the activity of specific enzymes of semen/spermatozoa are given. Moreover, the major factors that may influence the post-thaw viability and fertility of buffalo spermatozoa are examined in detail. In addition, suggestions for improvement in cryogenic procedures for buffalo spermatozoa are also given.

184 citations