Showing papers by "Myron S. Cohen published in 1981"
TL;DR: The results emphasized the importance of phagocytic cells in defense against fungi and the need for further evaluation of granulocyte transfusion therapy in compromised hosts in whom fungal infections develop.
328 citations
TL;DR: The objective was to establish a chronology of events leading to and following the initiation of Cellular Microbicidal Activity, as well as to investigate the relationship between O2formation and other events.
Abstract: II. Events Initiating Cellular Microbicidal Activity 566 A. Lysosomes and Lysosomal Degranulation .... 566 1 . Polymorphonuclear leukocytes (PMNs) ... 566 2. Eosinophils 567 B. The Respiratory Burst 567 1. Oxygen consumption 568 2. Activity of the hexose monophosphate shunt 568 3. Formation of superoxide (02-) and hydrogen peroxide (H202) ...... 569 4. Myeloperoxidase-mediated protein iodination 570 5. Chemiluminescence 572 6. Reduction of nitroblue tetrazolium dye .... 572 7. Relationship between O2formation and other events 573
251 citations
Journal Article•
TL;DR: It is suggested that thioglycollate-elicited macrophages have a vigorous oxidative metabolism but that recovery, and perhaps utilization, of O2 reduction products formed will depend on the conditions of incubation.
Abstract: The oxygen and glucose metabolism of peritoneal macrophages harvested from untreated mice (resident cells) and mice given an i.p. injection of thioglycollate broth (thioglycollate cells) were examined. Thioglycollate cells consumed approximately 3 times as much O2 at rest and during phagocytosis as resident cells, but oxygen reduction products (superoxide and hydrogen peroxide) could be recovered in only minimal amounts despite triggering by phagocytosis or exposure to PMA. Indirect evidence for the formation of oxygen reduction products such as O2- by thioglycollate cells was obtained by observation of the major pathways for glucose oxidation and NBT dye reduction. When thioglycollate cells were allowed to adhere to a glass surface O2- and H2O2 were easily recovered in the extracellular medium with a 20-fold increase above cells in suspension exposed to PMA. This study suggests that thioglycollate-elicited macrophages have a vigorous oxidative metabolism but that recovery, and perhaps utilization, of O2 reduction products formed will depend on the conditions of incubation. These events may be significant both for the study of parameters of macrophage "activation" in vitro as well as the function of these cells in vivo.
40 citations
TL;DR: Acetylation and succinylation of cytochrome c decrease its rate of reaction with superoxide, and forms the quantitative basis for the enhanced specificity of modified cy tochrome c for superoxide detection previously described by other investigators.
25 citations