Nabila Akram

Bio: Nabila Akram is an academic researcher. The author has contributed to research in topics: Cotton leaf curl virus & RAPD. The author has an hindex of 1, co-authored 1 publications receiving 1 citations.

Phylogenetic Analysis of First Generation Cotton Leaf Curl Virus (CLCuV) Resistant Cotton Varieties

Abstract: Cotton is a major cash crop of Pakistan. During the last few years, cotton production has been hampered by cotton leaf curl virus (CLCuV) disease. Cotton breeders successfully combated CLCuV by developing resistant varieties, however it resurfaced in 2001, all the varieties proving susceptible. This study was conducted to explore the genetic diversity among cotton varieties approved as first defense line against CLCuV using DNA markers. The four cotton varieties, CIM-448, CIM-1100, FH-900 and FH-901, were subjected to random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) finger printing assay to reveal genetic relatedness. A total of 110 RAPD primers were surveyed out of which 16 were found polymorphic. The size of 449 amplified loci ranged from 250 to 1500 bp with an average of 4.08 loci per primer. Similarity matrix revealed 96% similarity among the varieties assayed. For SSR genetic analysis, 92 primer pairs were surveyed and only six primers were found informative. A total ...

Role of QTL mapping to circumscribe various diseases in different crops with special emphasis on cotton.

Abstract: Cotton not only provides the world’s best natural spinnable fibre but also its seed is a rich source oil and protein, which is best for food and livestock feed. A biotic and biotic stresses always a serious threat to cotton production world widely. There is a demand of such cultivars which could oppose these stresses with wide genetic variability. Molecular breeding along with marker technology opened new ways in crop improvement. Genome mapping which is the identification of genomic regions linked with different traits; using construction of linkage map and quantitative trait loci (QTL) analysis can be used to identify resistant sources with the use of marker assistant selection (MAS). Improvements in control of many biotic diseases like verticillium (V) and Fusarium Wilt (FW), Reniform and Root-knot Nematodes had been made using molecular breeding. Cotton leaf curl disease (CLCuD) is the most distressing diseases of cotton in Pakistan, north-western areas of India and some parts of Africa which could be improved using these biotechnological tools. In many agricultural crops viral diseases like tomato yellow leaf curl virus (TYLCV), tomato mosaic virus (ToMV), tomato spotted wilt virus (TSWV), rice black-streaked virus (RBSV), Maize Streak Virus (MSV), Maize dwarf mosaic virus (MDMV), SCMV (Sugarcane mosaic virus), WSMV (Wheat streak mosaic virus), MCDV (Maize chlorotic dwarf virus), MMV (Maize mosaic virus), MFSV (Maize fine streak virus), Rice Stripe Virus (RSV), Rice yellow mottle virus (RYMV), rice black-streaked dwarf virus (RBSDV) and Barley Yellow Dwarf Virus (BYDV) had been studied using molecular markers. This Review study provides explanation of molecular markers involved in crop improvement especially for disease resistance in cotton and other field crop which could carry out to find out CLCuD resistant sources in upland cotton.