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Namrata Singh

Bio: Namrata Singh is an academic researcher from Indian Institute of Technology Bombay. The author has contributed to research in topics: Amyloid & Wound healing. The author has an hindex of 8, co-authored 19 publications receiving 184 citations. Previous affiliations of Namrata Singh include Indian Institute of Chemical Biology & University of Aveiro.

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TL;DR: This review focuses on the recent advances of amyloid-based biomaterials for cell adhesion and tissue engineering applications and suggests that, as long as amyloidal fibrils are not toxic, they can be utilized for cellAdhesion and tissues engineering applications.

87 citations

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TL;DR: Results showed that C. officinalis tincture potentiated wound healing by stimulating proliferation and migration of fibroblast in a PI3K‐dependent pathway, and the identified compounds are likely to be responsible for wound healing activity.
Abstract: Calendula officinalis, a member of the Asteraceae family, is a flowering plant and has been used for its antibacterial, antifungal, antiviral, antiinflammatory, anticancer and wound healing activity. The mode of action of C. officinalis tincture on wound healing is poorly understood. Here, we investigated the role of C. officinalis tincture (CDOT) on cell viability and wound closure. C. officinalis tincture stimulated both proliferation and migration of fibroblasts in a statistically significant manner in a PI3K-dependent pathway. The increase in phosphorylation of FAK (Tyr 397) and Akt (Ser 473) was detected after treatment of CDOT. Inhibition of the PI3K pathway by wortmannin and LY294002 decreased both cell proliferation and cell migration. HPLC-ESI MS revealed the presence of flavonol glycosides as the major compounds of CDOT. Altogether, our results showed that CDOT potentiated wound healing by stimulating proliferation and migration of fibroblast in a PI3K-dependent pathway, and the identified compounds are likely to be responsible for wound healing activity.

38 citations

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TL;DR: The biophysical properties of A53V are characterized for the first time, including the aggregation propensities, toxicity of aggregated species, and membrane binding capability, along with those of all familial mutations at the A53 position, to establish the disease-causing mechanism of A 53V in PD pathology.
Abstract: The involvement of α-synuclein (α-Syn) amyloid formation in Parkinson’s disease (PD) pathogenesis is supported by the discovery of α-Syn gene (SNCA) mutations linked with familial PD, which are known to modulate the oligomerization and aggregation of α-Syn. Recently, the A53V mutation has been discovered, which leads to late-onset PD. In this study, we characterized for the first time the biophysical properties of A53V, including the aggregation propensities, toxicity of aggregated species, and membrane binding capability, along with those of all familial mutations at the A53 position. Our data suggest that the A53V mutation accelerates fibrillation of α-Syn without affecting the overall morphology or cytotoxicity of fibrils compared to those of the wild-type (WT) protein. The aggregation propensity for A53 mutants is found to decrease in the following order: A53T > A53V > WT > A53E. In addition, a time course aggregation study reveals that the A53V mutant promotes early oligomerization similar to the cas...

33 citations

Journal ArticleDOI
TL;DR: This comprehensive proteomics based investigation revealed the dysregulation of 489 and 104 proteins in grades I and II of meningioma, respectively, along with the enrichment of several signalling pathways, which might play a crucial role in the manifestation of the disease.
Abstract: // Shabarni Gupta 1, * , Shuvolina Mukherjee 1, * , Parvez Syed 1, 2, * , Narendra Goud Pandala 1 , Saket Choudhary 3, 4 , Vedita Anand Singh 1 , Namrata Singh 1 , Heng Zhu 5 , Sridhar Epari 6 , Santosh B. Noronha 3 , Aliasgar Moiyadi 7 and Sanjeeva Srivastava 1 1 Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India 2 Department of Biochemistry/Biotechnology, University of Turku, Turun yliopisto, Finland 3 Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India 4 Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, USA 5 Department of Pharmacology and Molecular Sciences/High-Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA 6 Department of Pathology, Tata Memorial Centre, Mumbai, India 7 Department of Neurosurgery, Tata Memorial Centre, Mumbai, India * These authors have contributed equally to this work Correspondence to: Sanjeeva Srivastava, email: sanjeeva@iitb.ac.in Keywords: meningioma, autoantibody, protein array, brain tumors, HuPort screening Received: July 14, 2016 Accepted: March 11, 2017 Published: April 10, 2017 ABSTRACT Meningiomas are one of the most common tumors of the Central nervous system (CNS). This study aims to identify the autoantibody biomarkers in meningiomas using high-density human proteome arrays (~17,000 full-length recombinant human proteins). Screening of sera from 15 unaffected healthy individuals, 10 individuals with meningioma grade I and 5 with meningioma grade II was performed. This comprehensive proteomics based investigation revealed the dysregulation of 489 and 104 proteins in grades I and II of meningioma, respectively, along with the enrichment of several signalling pathways, which might play a crucial role in the manifestation of the disease. Autoantibody targets like IGHG4, CRYM, EFCAB2, STAT6, HDAC7A and CCNB1 were significantly dysregulated across both the grades. Further, we compared this to the tissue proteome and gene expression profile from GEO database. Previously reported upregulated proteins from meningioma tissue-based proteomics obtained from high-resolution mass spectrometry demonstrated an aggravated autoimmune response, emphasizing the clinical relevance of these targets. Some of these targets like SELENBP1 were tested for their presence in tumor tissue using immunoblotting. In the light of highly invasive diagnostic modalities employed to diagnose CNS tumors like meningioma, these autoantibody markers offer a minimally invasive diagnostic platform which could be pursued further for clinical translation.

24 citations

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TL;DR: In this article, the Diorganotin dichloride-phosphonic diamide complex, [Ph2SnCl2(tBuP(O)(NHiPr)2)2] (1), is prepared by the addition of two equivalents of tBuP to one equivalents of Ph2Cl2 either in the presence or absence of triethylamine.

17 citations


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TL;DR: The present review delineates, in short, the preparation, properties, and applications of different polymer and peptide hydrogels prepared in the past few years.
Abstract: In this review, we focus on the very recent developments on the use of the stimuli responsive properties of polymer hydrogels for targeted drug delivery, tissue engineering, and biosensing utilizing their different optoelectronic properties. Besides, the stimuli-responsive hydrogels, the conducting polymer hydrogels are discussed, with specific attention to the energy generation and storage behavior of the xerogel derived from the hydrogel. The electronic and ionic conducting gels have been discussed that have applications in various electronic devices, e.g., organic field effect transistors, soft robotics, ionic skins, and sensors. The properties of polymer hybrid gels containing carbon nanomaterials have been exemplified here giving attention to applications in supercapacitors, dye sensitized solar cells, photocurrent switching, etc. Recent trends in the properties and applications of some natural polymer gels to produce thermal and acoustic insulating materials, drug delivery vehicles, self-healing material, tissue engineering, etc., are discussed. Besides the polymer gels, peptide gels of different dipeptides, tripeptides, oligopeptides, polypeptides, cyclic peptides, etc., are discussed, giving attention mainly to biosensing, bioimaging, and drug delivery applications. The properties of peptide-based hybrid hydrogels with polymers, nanoparticles, nucleotides, fullerene, etc., are discussed, giving specific attention to drug delivery, cell culture, bio-sensing, and bioimaging properties. Thus, the present review delineates, in short, the preparation, properties, and applications of different polymer and peptide hydrogels prepared in the past few years.

228 citations

Journal ArticleDOI
TL;DR: An extensive survey of literature reporting the use of medical plants and plant-based products for cutaneous wounds describes the active ingredients, bioactivities, clinical uses, formulations, methods of preparation, and clinical value of 36 medical plant species.
Abstract: Cutaneous wound healing is the process by which skin repairs itself. It is generally accepted that cutaneous wound healing can be divided into 4 phases: haemostasis, inflammation, proliferation, and remodelling. In humans, keratinocytes re-form a functional epidermis (reepithelialization) as rapidly as possible, closing the wound and reestablishing tissue homeostasis. Dermal fibroblasts migrate into the wound bed and proliferate, creating “granulation tissue” rich in extracellular matrix proteins and supporting the growth of new blood vessels. Ultimately, this is remodelled over an extended period, returning the injured tissue to a state similar to that before injury. Dysregulation in any phase of the wound healing cascade delays healing and may result in various skin pathologies, including nonhealing, or chronic ulceration. Indigenous and traditional medicines make extensive use of natural products and derivatives of natural products and provide more than half of all medicines consumed today throughout the world. Recognising the important role traditional medicine continues to play, we have undertaken an extensive survey of literature reporting the use of medical plants and plant-based products for cutaneous wounds. We describe the active ingredients, bioactivities, clinical uses, formulations, methods of preparation, and clinical value of 36 medical plant species. Several species stand out, including Centella asiatica, Curcuma longa, and Paeonia suffruticosa, which are popular wound healing products used by several cultures and ethnic groups. The popularity and evidence of continued use clearly indicates that there are still lessons to be learned from traditional practices. Hidden in the myriad of natural products and derivatives from natural products are undescribed reagents, unexplored combinations, and adjunct compounds that could have a place in the contemporary therapeutic inventory.

188 citations

Journal ArticleDOI
TL;DR: The structural and functional aspects of α-Syn and role of potential factors that may contribute to the underlying mechanism of synucleinopathies are focused on to identify novel targets and develop specific therapeutic strategies to combat Parkinson's and other protein aggregation related neurodegenerative diseases.

186 citations

Journal ArticleDOI
TL;DR: The n-hexanic and the ethanolic extracts from Calendula flowers influence the inflammatory phase by activating the transcription factor NF-κB and by increasing the amount of the chemokine IL-8 in human immortalized keratinocytes.

89 citations

Journal ArticleDOI
Hilal A. Lashuel1
TL;DR: A critical analysis of the data and claims used to investigate the structure, composition, and architecture of LBs from postmortem brain tissues of individuals with PD or other synucleinopathies suggests that lipid membrane fragments and distorted organelles together with a non-fibrillar form of αSyn are the main structural building blocks for the formation of Lewy pathology.

69 citations