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Author

Nana Tchabo

Other affiliations: Overlook Medical Center
Bio: Nana Tchabo is an academic researcher from Atlantic Health System. The author has contributed to research in topics: Endometrial cancer & Sentinel lymph node. The author has an hindex of 3, co-authored 5 publications receiving 331 citations. Previous affiliations of Nana Tchabo include Overlook Medical Center.

Papers
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Journal ArticleDOI
07 Jan 2014-PLOS ONE
TL;DR: In insight into the relationship between tumor dissemination and metabolic attributes of human cancer stem cells, spheroid cells were found to be enriched for cells with cancer stem cell-like characteristics and contributed to tumor generation, progression and chemotherapy resistance.
Abstract: Cells with sphere forming capacity, spheroid cells, are present in the malignant ascites of patients with epithelial ovarian cancer (EOC) and represent a significant impediment to efficacious treatment due to their putative role in progression, metastasis and chemotherapy resistance. The exact mechanisms that underlie EOC metastasis and drug resistance are not clear. Understanding the biology of sphere forming cells may contribute to the identification of novel therapeutic opportunities for metastatic EOC. Here we generated spheroid cells from human ovarian cancer cell lines and primary ovarian cancer. Xenoengraftment of as few as 2000 dissociated spheroid cells into immune-deficient mice allowed full recapitulation of the original tumor, whereas >105 parent tumor cells remained non-tumorigenic. The spheroid cells were found to be enriched for cells with cancer stem cell-like characteristics such as upregulation of stem cell genes, self-renewal, high proliferative and differentiation potential, and high aldehyde dehydrogenase (ALDH) activity. Furthermore, spheroid cells were more aggressive in growth, migration, invasion, scratch recovery, clonogenic survival, anchorage-independent growth, and more resistant to chemotherapy in vitro. 13C-glucose metabolic studies revealed that spheroid cells route glucose predominantly to anaerobic glycolysis and pentose cycle to the detriment of re-routing glucose for anabolic purposes. These metabolic properties of sphere forming cells appear to confer increased resistance to apoptosis and contribute to more aggressive tumor growth. Collectively, we demonstrated that spheroid cells with cancer stem cell-like characteristics contributed to tumor generation, progression and chemotherapy resistance. This study provides insight into the relationship between tumor dissemination and metabolic attributes of human cancer stem cells and has clinical implications for cancer therapy.

305 citations

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TL;DR: Morcellation was associated with substantially higher risk of abdominopelvic recurrence and lower disease-free survival and further research on uterine morcellation should focus on decision and cost-benefit analyses.

56 citations

Journal ArticleDOI
TL;DR: RSLND using methylene blue cervical injection can identify SLN in most patients with EC, and IHC ultrastaging improves the detection of node positive disease when compared to traditional pathological evaluation.

24 citations


Cited by
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Journal ArticleDOI
TL;DR: This review attempts to summarize the various 3D culture systems, with an emphasis on the most well characterized and widely applied model - multicellular tumor spheroids.

603 citations

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TL;DR: 10 important aspects of tumor angiogenesis and the pathological tumor vasculature which would be well suited as targets for anti-angiogenic therapy are identified and 10 plant-derived compounds could be combined to constitute a broader acting and more effective inhibitory cocktail at doses that would not be likely to cause excessive toxicity.

387 citations

Journal ArticleDOI
TL;DR: Determining the role of cancer stem cell metabolism in carcinogenesis has become a major focus in cancer research, and substantial efforts are conducted towards discovering clinical targets.
Abstract: Cancer is now viewed as a stem cell disease. There is still no consensus on the metabolic characteristics of cancer stem cells, with several studies indicating that they are mainly glycolytic and others pointing instead to mitochondrial metabolism as their principal source of energy. Cancer stem cells also seem to adapt their metabolism to microenvironmental changes by conveniently shifting energy production from one pathway to another, or by acquiring intermediate metabolic phenotypes. Determining the role of cancer stem cell metabolism in carcinogenesis has become a major focus in cancer research, and substantial efforts are conducted towards discovering clinical targets.

343 citations

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TL;DR: A well-defined soluble basement membrane extract, termed BME/Matrigel, prepared from an epithelial tumor is similar in content to authentic basement membrane, and forms a hydrogel at 24-37°C and has widespread use in assays and in models that improve understanding of tumor biology and help define therapeutic approaches.

338 citations

Journal ArticleDOI
TL;DR: Sentinel lymph node (SLN) mapping has recently been introduced into the surgical staging of endometrial cancer with the goal to reduce morbidity associated with comprehensive lymphadenectomy, yet obtain prognostic information from lymph node status.

273 citations