Nannapat Wannaphruek

Bio: Nannapat Wannaphruek is an academic researcher from Rangsit University. The author has contributed to research in topics: Transdermal & Pectin. The author has an hindex of 2, co-authored 2 publications receiving 19 citations.

Transdermal Delivery of Nicotine Using Pectin Isolated from Durian Fruit-Hulls-Based Polymer Blends as a Matrix Layer

Abstract: Many natural polymers with various chemical structures are used to prepare transdermal patches. Pectin is a one interesting type of polymer because it can control drug release when used in transdermal patches. In Thailand, the waste from durian fruit-hulls is a major problem for the environment. However, the pectin from it can be isolated under acid conditions and used to prepare transdermal patches for nicotine delivery which has not yet been reported. As the isolated pectin is a natural polymer, the film made from isolated pectin is a brittle; therefore, adding a low protein natural rubber latex (LPNRL) polymer was needed to increase its flexibility. The transdermal patches were amorphous and had Tg values ranging from 81.0 to 93.3 °C. Moisture uptake, swelling ratio, and erosion values of the patches were significantly decreased after addition of LPNRL, which resulted in low hydrophilicity. The in vitro release and permeation of nicotine depends on the hydrophilicity of the patches. The kinetic models for in vitro release and permeation of nicotine were Higuchi model and zero order, respectively. In conclusion, pectin isolated from fruit-hulls of Mon Thong durians is an effective polymer to control the release of nicotine. It also is an option that could solve the environmental problems caused by durian fruit-hulls waste.

Use of Isolated Pectin from a Cissampelos pareira-Based Polymer Blend Matrix for the Transdermal Delivery of Nicotine

Abstract: Pectin is a natural biopolymer, and a major component of a complex heterogeneous polysaccharide found in the primary cell walls and middle lamella of plant tissues. This paper used pectin isolated from Cissampelos pareira (Krueo Ma Noy) leaves to prepare the matrix layer for nicotine transdermal patches. However, the patch was a brittle film, thus, deproteinized natural rubber latex (DNRL) was blended to improve flexibility of the patch. Here we present for the first time a preparation study exploring the suitability of isolated pectin blends to serve as drug carriers and the mechanism controlling the release patterns of nicotine. The hydrophilicity of the patches was found to decrease when increasing the DNRL ratio. Differential scanning calorimetry and X-ray diffraction experiments were used to characterize the interactions between the investigated drugs and the matrix polymers. In vitro studies showed that the isolated pectin blends were an effective matrix for controlled nicotine release. The release and permeation patterns of nicotine depend on the hydrophilicity of the patches. The kinetic models of nicotine were found to be a Higuchi model and zero order for in vitro release and skin permeation, respectively.

Impacts of high hydrostatic pressure processing on the structure and properties of pectin

Abstract: To understand the impacts of high hydrostatic pressure (HHP) processing on the microstructure and properties of pectin, an aqueous solution of apple pectin (50 g/L) was treated at 200, 400, and 600 MPa for 30 min. The chemical structure and properties of resultant pectin were characterized and compared with those of native pectin (0.1 MPa). The contents of α-1,4-linked d -galacturonic acid and reducing sugar in pectin did not change after HHP, whereas the degree of esterification of pectin increased slightly. Fourier transform infrared spectroscopy showed that no chemical conversion of pectin occurred during HHP process. For the first time, molecular dynamics simulation revealed that the hydrophobic hydration of the methyl group dramatically improved under HHP, thereby enhancing pectin dissolution in water. In turn, this result increased the aqueous pectin solution's apparent viscosity and stability, which are very important for fruit- and vegetable-based products containing pectin.

Development of Biodegradable Cosmetic Patch Using a Polylactic Acid/Phycocyanin-Alginate Composite.

Abstract: The usage of non-degradable polymer as the main matrix for a cosmetic patch raises concern, as it can cause environmental pollution when discarded in landfill. Thus, biodegradable polylactic acid (PLA) was chosen in this study, as PLA has non-toxic properties and similar mechanical properties to conventional plastic materials. An active ingredient in a cosmetic patch serves the purpose of providing beneficial ingredients to the skin; therefore, phycocyanin, an extract from spirulina, was chosen, as it possesses antioxidant and anti-inflammatory properties. Alginate was also incorporated with the phycocyanin for fabrication onto the PLA matrix. A preliminary study was first carried out to identify the antioxidant properties and cytotoxic effect of the phycocyanin on skin cells. It was observed that phycocyanin had no cytotoxic effect on the skin and showed good antioxidant activity. PLA/phycocyanin-alginate composite was fabricated using a solvent casting method, and optimization of preparation conditions (phycocyanin/alginate ratio, stirring time, and temperature) were carried out using the one-factor-at-a-time (OFAT) method with responses of elongation at break and releasing properties. Attenuated total reflectance (ATR)-FTIR analysis was also conducted to further analyze the functional group of the composites. Surface morphologies were observed for samples before and after the releasing test. From the analyses conducted, PLA/phycocyanin-alginate composite prepared at a phycocyanin/alginate ratio of 40/60 for 20 h at 20 °C gave the best properties in terms of flexibility of film and releasing properties of phycocyanin.

Gamma irradiation synthesis of pectin- based biohydrogels for removal of lead cations from simulated solutions

Abstract: Abstract Bio-based hydrogels (denoted as PC-PAAc/GA) comprised of Pectin (PC) and polyacrylic acid (PAAc) reinforced with different ratios of gallic acid (GA) were prepared by gamma radiation at irradiation dose 20 kGy. The prepared hydrogels were investigated by different analytical tools. The swelling performance was studied versus time, pH of the medium and gallic acid content. The experimental data depicted that the swelling increases with pH of medium until the equilibrium of swelling after 350 min. The maximum swelling was attained at pH10 for both PC-PAAc and PC-PAA/GA1.5. Also, the data reveal that the incorporation of GA in the hydrogel matrix enhanced the swelling performance of the hydrogel up to an optimum value of GA, i.e. PC-PAA/GA1.5. Further increase in GA concentration leads to formation of a highly crosslinked structure with reduced swelling. The results demonstrated that the prepared hydrogels displayed excellent antibacterial activity against gram + ve bacteria (E.coli) and gram-ve bacteria (S.aureus) . This potent antimicrobial activity is mainly originated from GA which was proved as a strong antibacterial agent. Moreover, the removal performance of the investigated hydrogels was verified towards Pb +2 cation as one of the most poisonous heavy metals. The data revealed that the maximum removal percentage of Pb (II) was attained by PC-PAAc/GA1.5 hydrogel (90 mg g −1 ). The correlation coefficients of the Langmuir model are too higher than that of the Freundlich model that assumed the adsorption of lead cations is mainly a chemical process.