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Natalya V. Boikova

Bio: Natalya V. Boikova is an academic researcher. The author has contributed to research in topics: Microbial inoculant & Rhizobacteria. The author has an hindex of 1, co-authored 1 publications receiving 19 citations.

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TL;DR: This study is the first to report that Azospirillum inoculation of potato microclones not only improves the quality of planting material produced in vitro but also significantly increases minituber yield through enhancing plant adaptive capacity in the field.
Abstract: Microclonal propagation in vitro is being actively used in the production of healthy planting material of food and ornamental plants. However, it needs further improvement to increase the growth rates of microclones in vitro and enhance regenerant survivability ex vitro. A promising approach to this end could be inoculating in vitro-micropropagated plants with plant growth-promoting rhizobacteria, specifically Azospirillum. However, the influence of Azospirillum inoculation on microclone behavior throughout the production process, including plant adaptation ex vitro and food crop productivity, has been underinvestigated. In this study, in vitro-growing potato (Solanum tuberosum L.) microclones were inoculated with Azospirillum brasilense strain Sp245. The microclones were then grown on in soil in the greenhouse and field, with the experiment lasting for 120 days. Root-associated bacteria were identified immunochemically, and the mitotic index of root meristematic cells was determined by a cytological method. The plant morphological parameters determined were shoot length, number of nodes per shoot, number of roots per plant, maximal root length, leaf area, percentage of surviving plants in the soil, and tuber yield and weight. Our results show that bacterial inoculation of potato microclones in vitro enhances plant adaptive capacity ex vitro and increases minituber yield. The percent survival index of field-grown inoculated plants was 1.5-fold greater than that of uninoculated plants. The overall tuber weight per plant was more than 30 % greater in the inoculated plants than it was in the control ones. For all cultivars on average, tuber yield per square meter increased by more than 45 % as a result of inoculation in vitro. This study is the first to report that Azospirillum inoculation of potato microclones not only improves the quality of planting material produced in vitro but also significantly increases minituber yield through enhancing plant adaptive capacity in the field.

28 citations


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TL;DR: It is shown that most Azospirillum strains cannot utilize sucrose as the sole carbon source and that their use to inoculate in vitro-grown plants does not lead to bacterial growth in the culture medium.
Abstract: The response of potato microplants to Azospirillum inoculation is highly variable both in vitro and ex vitro. Plant inoculation with plant-growth-promoting rhizobacteria (PGPR) is widely used to increase the effectiveness of clonal micropropagation. Azospirillum rhizobacteria are model subjects to investigate associative plant–microbe interactions. Here we show that most Azospirillum strains cannot utilize sucrose as the sole carbon source and that their use to inoculate in vitro-grown plants does not lead to bacterial growth in the culture medium. Of the eleven surveyed strains, seven gave a significant increase in at least one growth variable of in vitro-grown potato (Solanum tuberosum L. cv. Nevsky) microplants. Inoculation with six strains led to better survival of the microplants in soil. Only with three strains (A. brasilense Sp245, SR80, and A. halopraeferens Au4T) did inoculation in vitro significantly promote plant growth ex vitro. The inoculation results were correlated with the biochemical activity of the strains. Indole-3-acetic acid production ranged from 3.74 μg ml−1 with A. brasilense S27 to 87.3 μg ml−1 with A. brasilense Sp245. Active indole-3-acetic acid producers, but not nitrogen fixers, were better plant-growth-promoters. Inoculation in vitro with A. brasilense strains Sp245 and SR80 can be recommended for increasing the effectiveness of clonal micropropagation of potato.

25 citations

Journal ArticleDOI
24 Aug 2020
TL;DR: The effects of inoculating the culture medium of potato microplants grown in vitro with Azospirillum brasilense Sp245 or Ochrobactrum cytisi IPA7.2 with rhizobacteria known to increase plant resistance to abiotic and other stresses were studied.
Abstract: Water deficits inhibit plant growth and decrease crop productivity. Remedies are needed to counter this increasingly urgent problem in practical farming. One possible approach is to utilize rhizobacteria known to increase plant resistance to abiotic and other stresses. We therefore studied the effects of inoculating the culture medium of potato microplants grown in vitro with Azospirillum brasilense Sp245 or Ochrobactrum cytisi IPA7.2. Growth and hormone content of the plants were evaluated under stress-free conditions and under a water deficit imposed with polyethylene glycol (PEG 6000). Inoculation with either bacterium promoted the growth in terms of leaf mass accumulation. The effects were associated with increased concentrations of auxin and cytokinin hormones in the leaves and stems and with suppression of an increase in the leaf abscisic acid that PEG treatment otherwise promoted in the potato microplants. O. cytisi IPA7.2 had a greater growth-stimulating effect than A. brasilense Sp245 on stressed plants, while A. brasilense Sp245 was more effective in unstressed plants. The effects were likely to be the result of changes to the plant’s hormonal balance brought about by the bacteria.

21 citations

Journal ArticleDOI
TL;DR: The taxonomic position of Ochrobactrum cytisi strain IPA7.2 was clarified and its tolerance for salinity, high temperature, and glyphosate pollution, and the strain’s potential to promote the growth of potato microplants was tested.
Abstract: Bacteria in natural associations with agricultural crops are promising for use in the improvement of clonal micropropagation of plants. We clarified the taxonomic position of Ochrobactrum cytisi strain IPA7.2 and investigated its tolerance for salinity, high temperature, and glyphosate pollution. We also tested the strain’s potential to promote the growth of potato (Solanum tuberosum L.) microplants. Using the IPA7.2 draft genome (no. NZ_MOEC00000000), we searched for housekeeping genes and also for the target genes encoding glyphosate tolerance and plant-growth-promoting ability. A multilocus sequence analysis of the gap, rpoB, dnaK, trpE, aroC, and recA housekeeping genes led us to identify isolate IPA7.2 as O. cytisi. The strain tolerated temperatures up to 50 °C and NaCl concentrations up to 3–4%, and it produced 8 µg ml−1 of indole-3-acetic acid. It also tolerated 6 mM glyphosate owing to the presence of type II 5-enolpyruvylshikimate-3-phosphate synthase. Finally, it was able to colonize the roots and tissues of potato microplants, an ability preserved by several generations after subculturing. We identified the development phase of potato microplants that was optimal for inoculation with O. cytisi IPA7.2. Inoculation of in vitro-grown 15-day-old microplants increased the mitotic index of root meristem cells (by 50%), the length of shoots (by 34%), the number of leaves (by 7%), and the number of roots (by 16%). Under ex vitro conditions, the inoculated plants had a greater leaf area (by 77%) and greater shoot and root dry weight (by 84 and 61%, respectively) than did the control plants. We recommend O. cytisi IPA 7.2 for use in the growing of potato microplants to improve the production of elite seed material.

20 citations

Journal ArticleDOI
TL;DR: The results of this research indicate the bacterial mixture used in this study has a high potential to support satisfactory potato yields under dry conditions and under low levels of infection by foliar fungal diseases and attacks by folian insects.
Abstract: In 2017, we conducted a field experiment to test the influence of a mixture of two plant growth-promoting bacteria (Pseudomonas fluorescens and Azospirillum brasilense) on the yield of three potato...

19 citations