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Nathan J Rollins
Researcher at Harvard University
Publications - 12
Citations - 249
Nathan J Rollins is an academic researcher from Harvard University. The author has contributed to research in topics: Biology & Immune system. The author has an hindex of 5, co-authored 9 publications receiving 122 citations. Previous affiliations of Nathan J Rollins include Wyss Institute for Biologically Inspired Engineering.
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Journal ArticleDOI
Inferring protein 3D structure from deep mutation scans.
Nathan J Rollins,Kelly P Brock,Frank J. Poelwijk,Michael A Stiffler,Nicholas P. Gauthier,Chris Sander,Chris Sander,Debora S. Marks,Debora S. Marks +8 more
TL;DR: It is found that the strongest epistatic pairings from genetic screens of three proteins, a ribozyme and a protein interaction reveal 3D contacts within and between macromolecules, suggesting that genomics-based techniques can efficiently predict 3D structure.
Journal ArticleDOI
Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact.
Melissa A Chiasson,Nathan J Rollins,Jason J. Stephany,Katherine A. Sitko,Kenneth A. Matreyek,Marta Verby,Song Sun,Frederick P. Roth,Daniel DeSloover,Debora S. Marks,Allan E. Rettie,Douglas M. Fowler +11 more
TL;DR: Functionally constrained regions of the protein define the active site, and it is found that, of four conserved cysteines putatively critical for function, only three are absolutely required.
Journal ArticleDOI
PDBe-KB: collaboratively defining the biological context of structural data
Mihaly Varadi,Stephen Anyango,David R. Armstrong,John M. Berrisford,Preeti Choudhary,Mandar Deshpande,Nurul Nadzirin,Sreenath Nair,Lukáš Pravda,Ahsan Tanweer,Bissan Al-Lazikani,Claudia Andreini,Geoffrey J. Barton,David Bednar,Karel Berka,Tom L. Blundell,Kelly P Brock,José María Carazo,Jiri Damborsky,Alessia David,Sucharita Dey,Roland L. Dunbrack,Juan Fernandez Recio,Franca Fraternali,Toby J. Gibson,Manuela Helmer-Citterich,David Hoksza,Thomas A. Hopf,David Jakubec,Natarajan Kannan,Radoslav Krivak,Manjeet Kumar,Emmanuel D. Levy,Nir London,Jose Ramon Macias,Madhusudhan M Srivatsan,Debora S. Marks,Lennart Martens,Stuart A McGowan,Jake E McGreig,Vivek Modi,R. Gonzalo Parra,Gerardo Pepe,Damiano Piovesan,Jaime Prilusky,Valeria Putignano,Leandro G Radusky,Pathmanaban Ramasamy,Atilio O Rausch,Nathalie Reuter,Luis A Rodriguez,Nathan J Rollins,Antonio Rosato,Pawel Rubach,Luis Serrano,Gulzar Singh,Petr Škoda,Carlos Oscar S. Sorzano,Jan Stourac,Joanna I. Sulkowska,Radka Svobodová,Natalia Tichshenko,Silvio C. E. Tosatto,Wim F. Vranken,Mark N. Wass,Dandan Xue,Daniel Zaidman,Janet M. Thornton,Michael J.E. Sternberg,Christine A. Orengo,Sameer Velankar +70 more
Journal ArticleDOI
Chimeric Fatty Acyl-Acyl Carrier Protein Thioesterases Provide Mechanistic Insight into Enzyme Specificity and Expression
Marika Ziesack,Marika Ziesack,Nathan J Rollins,Nathan J Rollins,Aashna Shah,Brendon Dusel,Gordon D. Webster,Pamela A. Silver,Pamela A. Silver,Jeffrey C. Way +9 more
TL;DR: The engineering of chimeric enzymes and characterizing their microbial activity and chain-length specificity suggested mechanistic insights into TE functions and also generated thioesterases with potentially useful properties that may inform a rational engineering of TEs to allow alkyl chain length control.
Journal ArticleDOI
Exploring targeting peptide-shell interactions in encapsulin nanocompartments.
Wiggert J. Altenburg,Nathan J Rollins,Nathan J Rollins,Pamela A. Silver,Pamela A. Silver,Tobias W. Giessen +5 more
TL;DR: In this article, the authors characterize and engineer TP-shell interactions in the Thermotoga maritima and Myxococcus xanthus encapsulin systems using force-field modeling and particle fluorescence measurements.