N
Neil B. Chilton
Researcher at University of Saskatchewan
Publications - 191
Citations - 7590
Neil B. Chilton is an academic researcher from University of Saskatchewan. The author has contributed to research in topics: Internal transcribed spacer & Ribosomal DNA. The author has an hindex of 47, co-authored 190 publications receiving 7149 citations. Previous affiliations of Neil B. Chilton include Institut national de la recherche agronomique & Public Health Agency of Canada.
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Journal ArticleDOI
Rapid sequencing of rDNA from single worms and eggs of parasitic helminths
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Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Strongyloidea)
TL;DR: Differences between these 3 species of H. Macropi in their recognition sites for restriction endonucleases, indicates that a PCR-RFLP approach could be used, in conjunction with allozyme electrophoresis, to establish how many species are present within the H. macropi complex.
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Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation
Robin B. Gasser,Min Hu,Neil B. Chilton,Bronwyn E. Campbell,Aaron J Jex,Domenico Otranto,Claudia Cafarchia,Ian Beveridge,Xing-Quan Zhu,Xing-Quan Zhu +9 more
TL;DR: The present protocol describes the SSCP method of analysis, including all steps from the small-scale isolation of genomic DNA and PCR amplification of target sequences, through to the gel-based separation of amplicons and scanning for mutations by SSCP.
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Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences.
TL;DR: PCR-based restriction fragment length polymorphism and single-strand conformation polymorphism methods were established for the unequivocal delineation of the three species and should provide valuable tools for studying the life-cycle, transmission pattern(s), population structure and diagnosis of anisakiasis in humans and animals.
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Differences in the second internal transcribed spacer (ribosomal DNA) between five species of Trichostrongylus (Nematoda: Trichostrongylidae).
TL;DR: The second internal transcribed spacer (ITS-2) of the ribosomal DNA of 5 species of Trichostrongylus has been sequenced and the recognition sites for several enzymes located within the regions of sequence homology for the five species are of important diagnostic value for species identification.