N
Neil Cooch
Researcher at Wistar Institute
Publications - 17
Citations - 8456
Neil Cooch is an academic researcher from Wistar Institute. The author has contributed to research in topics: Pembrolizumab & Medicine. The author has an hindex of 11, co-authored 12 publications receiving 7981 citations.
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Journal ArticleDOI
The Microprocessor complex mediates the genesis of microRNAs
Richard I. Gregory,Kai Ping Yan,Govindasamy Amuthan,Thimmalah Chendrimada,Behzad Doratotaj,Neil Cooch,Ramin Shiekhattar +6 more
TL;DR: In vivo knock-down and in vitro reconstitution studies revealed that both components of this smaller complex, termed Microprocessor, are necessary and sufficient in mediating the genesis of miRNAs from the primary miRNA transcript.
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TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing.
Thimmaiah P. Chendrimada,Richard I. Gregory,Easwari Kumaraswamy,Easwari Kumaraswamy,Jessica Norman,Neil Cooch,Kazuko Nishikura,Ramin Shiekhattar +7 more
TL;DR: It is shown that TRBP, which contains three double-stranded, RNA-binding domains, is an integral component of a Dicer-containing complex, and depletion of the Dicer–TRBP complex via exogenously introduced siRNAs diminished RISC-mediated reporter gene silencing.
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Human RISC Couples MicroRNA Biogenesis and Posttranscriptional Gene Silencing
TL;DR: It is shown that RISC is composed of Dicer, the double-stranded RNA binding protein TRBP, and Argonaute2 and it is demonstrated that this complex can cleave target RNA using precursor microRNA (pre-miRNA) hairpin as the source of siRNA.
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An essential role for CoREST in nucleosomal histone 3 lysine 4 demethylation
TL;DR: In vitro reconstitution of the BHC complex using recombinant subunits reveals an essential role for the REST corepressor CoREST, not only in stimulating dem methylation on core histones but also promoting demethylation of nucleosomal substrates.
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Integrator, a Multiprotein Mediator of Small Nuclear RNA Processing, Associates with the C-Terminal Repeat of RNA Polymerase II
David Baillat,Mohamed-Ali Hakimi,Anders M. Näär,Ali Shilatifard,Neil Cooch,Ramin Shiekhattar +5 more
TL;DR: An RNA polymerase II complex that contains at least 12 novel subunits, termed the Integrator, in addition to core RNAPII subunits is described, which shows that Integrator is recruited to the U1 and U2 snRNA genes and mediates the snRNAs' 3' end processing.