Showing papers by "Nianbai Fang published in 2011"
Journal Article•
TL;DR: Alismatis rhizome may act by decreasing the liver synthesis of cholesterol, rather than by increasing the cholesterol catabolism, in mice fed high-fat diet and confirmed the efficacy of AR in the treatment of hyperlipidemia.
Abstract: OBJECTIVES
To investigate the effect of Alismatis rhizome (AR) extract on lipid profile in mice fed high-fat diet.
METHODS
The study was performed in Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, Wuhan, China, between December 2009 and June 2010. Forty male Kunming mice (8-week-old) were randomly divided into 4 groups and were treated for 4 weeks: Group 1: normal control, Group 2: high-fat control, Group 3: positive control and Group 4: AR 2.26 g/kg. The hypolipidemic effects of AR were evaluated by serum lipids, liver lipids, and reverse transcriptase polymerase chain reaction. Serum aminotransferases and histopathological changes were also measured.
RESULTS
Alismatis rhizome treatment resulted in an obvious decrease in serum and liver cholesterol, triglyceride along with elevated serum high-density lipoprotein cholesterol in hyperlipidemic mice. The histopathological results showed that adipose vacuoles in AR treated mice liver were almost identical to those of normal control mice. Serum alanine transaminase, aspartate aminotransferase and the relative liver weight in AR treated mice were decreased significantly. Alismatis rhizome substantially decreased the mRNA expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (Hmgcr), while the expressions of sterol regulatory element binding factor 2 (Srebf2) and cholesterol 7alpha-hydroxylase (Cyp7a1) were marginally affected.
CONCLUSIONS
These results confirmed the efficacy of AR in the treatment of hyperlipidemia. Alismatis rhizome may act by decreasing the liver synthesis of cholesterol, rather than by increasing the cholesterol catabolism.
39 citations
TL;DR: The data suggest that increased hepatic CYP3A expression and inducibility following SPI feeding involves recruitment of PXR to its response element and suggests that soy consumption has potential effects on metabolism and transport of a wide variety of drugs and on bile acid homeostasis via proteins regulated by this transcription factor.
Abstract: Previous studies and Expt. 1 of the current study demonstrate that diets made with soy protein isolate (SPI) enhance the glucocorticoid-inducibility of hepatic cytochrome P450 (CYP)3A-dependent monooxygenase activities (P 200-fold in SPI-fed males and females at PND25 compared with a 100-fold increase in CAS-fed rats (P < 0.05). The DEX-induced increase in CYP3A1 mRNA in SPI-fed rats on PND60 was also greater than that in CAS-fed rats. The induction by DEX of CYP3A2 mRNA was 1- to 3-fold greater in rats fed SPI compared with those fed CAS on PND25 (P < 0.05). Quantitation of newly synthesized CYP3A1 RNA transcripts by nuclear run-on analysis demonstrated a greater rate of basal transcription in SPI-fed compared with CAS-fed rats on PND60 accompanied by greater binding of the pregnane X receptor (PXR) to a response element on the CYP3A1 promoter in SPI-fed compared with CAS-fed rats (P < 0.05). These data suggest that increased hepatic CYP3A expression and inducibility following SPI feeding involves recruitment of PXR to its response element and suggests that soy consumption has potential effects on metabolism and transport of a wide variety of drugs and on bile acid homeostasis via proteins regulated by this transcription factor.
16 citations