Showing papers by "Nilesh J. Samani published in 1987"
TL;DR: The histological localization of embryonic and fetal tissues whose cells express the genes coding for insulin-like growth factors I and II in the developing rat is described and no IGF mRNA was demonstrable in ectodermally derived structures, including the central and peripheral nervous systems as well as the skin and its derivatives.
Abstract: We describe the histological localization of embryonic and fetal tissues whose cells express the genes coding for insulin-like growth factors I and II (IGF-I and IGF-II) in the developing rat Our studies span the period between early somite stages and full term We have used oligodeoxyribonucleotide probes and obtained results which are both topographically precise and highly reproducible The gene coding for IGF-II is predominant throughout development It is strongly expressed in the liver and yolk sac A variety of other tissues also expresses the IGF-II gene, especially many mesodermally derived structures in the process of differentiation Many tissues do not express IGF genes Thus no IGF mRNA was demonstrable in ectodermally derived structures, including the central and peripheral nervous systems as well as the skin and its derivatives
191 citations
TL;DR: With the more sensitive RNAse protection technique, the initial results indicate more widespread expression of renin, messenger RNA (mRNA) being detected in aorta, adrenal, heart, lung and spleen.
Abstract: Total cellular RNA and poly A+ RNA isolated from various tissues of Wistar-Kyoto (WKY) rats were screened for renin-specific messenger RNA by Northern blotting and RNAse protection. Northern blot analysis, carried out with a copy DNA (cDNA) probe of mouse submandibular gland renin, demonstrated a single RNA species of 1.8 kb in the kidneys of WKY rats. Other tissues screened by this technique were negative. With the more sensitive RNAse protection technique, our initial results indicate more widespread expression of renin, messenger RNA (mRNA) being detected in aorta, adrenal, heart, lung and spleen.
41 citations