Nina Dwi Yulia

Bio: Nina Dwi Yulia is an academic researcher from Center for Plant Conservation. The author has contributed to research in topics: Population & Velamen. The author has an hindex of 4, co-authored 7 publications receiving 28 citations. Previous affiliations of Nina Dwi Yulia include Indonesian Institute of Sciences.

Morphology, anatomy, and mycorrhizal fungi colonization in roots of epiphytic orchids of Sempu Island, East Java, Indonesia

Abstract: Nurfadilah S, Yulia ND, Ariyanti EE. 2016. Morphology, anatomy, and mycorrhizal fungi colonisation in roots of epiphytic orchids of Sempu Island, East Java, Indonesia. Biodiversitas 17: 592-603. Roots of orchids have important role for survival, adaptation, water and nutrient absorption, and as a place of symbiosis with mycorrhizal fungi. The present study aimed to investigate the morphology, anatomy, and mycorrhizal status in roots of orchids of Sempu Island, Indonesia (Ascochilus emarginatus, Taeniophyllum biocellatum, and Thrixspermum subulatum), in relation to their adaptation to their habitat of coastal forests of Sempu Island. These orchids have different morphological characters; Ascochilus emarginatus and Thrixspermum subulatum are leafy orchids, while Taeniophyllum biocellatum is a leafless orchid. The results showed that all orchids have small number of velamen layers (1-2 layers) as an adaptation to the relatively humid condition. Cell wall thickenings of velamen, exodermis, and endodermis are structural adaptation of all orchids to the relatively high intensity of illumination, to reduce water loss because of transpiration. Mycorrhizal fungi colonization which is important for nutrient acquisition occurs in cortical cells. All orchids have differences in their cell shape, size, and specific characters, such as chloroplasts. The leafless Taeniophyllum biocellatum has many chloroplasts in the cortical root cells that support the photosynthesis process, while A. emarginatus and T. subulatum are lack of chloroplasts in their cortical root cells.

Analysis of epiphytic orchid diversity and its host tree at three gradient of altitudes in Mount Lawu, Java

Abstract: Yulia ND, Budiharta S, Yulistyarini T (2011) Analysis of epiphytic orchid diversity and its host tree at three gradient of altitudes in Mount Lawu, Java. Biodiversitas 12: 225-228. The aim of this study was to observe epiphytic orchid diversity and their host trees at three different altitudes (1796, 1922 and 2041 m asl.) at southern part of Mount Lawu, District of Magetan, East Java. Line transect of 10 x 100 m was set up and then divided into ten plots (as replicates) of 10 x 10 m. At each plot, species name and number of individual of epiphytic orchids, and species name, number of individual and diameter at breast height (dbh) of host trees were recorded. The result showed that there were 19 species of epiphytic orchid recorded at the study sites. There were significantly different among gradient altitude in number of epiphytic orchid species (F = 3.7; df = (2, 27); P < 0.05). The highest number of species of epiphytic orchid was recorded at the altitude of 1922 m asl. (6.6 species/100 m2) while the highest number of individual was recorded at the altitude of 1796 m asl. (1337.7 individuals/100 m2). The study site at altitude of 1922 m asl. was recognized as the denser and richer in species of host trees (2.3 species/100 m2 and 3.5 individuals/100 m2 respectively). However, the highest basal area of host tree was recorded at the altitude of 2041 m asl. (4558 cm2/100 m2).

Epiphytic orchids and host trees diversity at Gunung Manyutan Forest Reserve, Wilis Mountain, Ponorogo, East Java

Abstract: Yulia ND, Budiharta S (2011). Epiphytic orchids and host trees diversity at Gunung Manyutan Forest Reserve, Wilis Mountain, Ponorogo, East Java. Biodiversitas 12: 22-27. Natural forests in Wilis Mountain have been destroyed by forest fires, landslides and illegal logging. As a consequence, biological diversity in this area is threatened by local extinctions, particularly of orchid species. This study was aimed to explore, document and analyze the diversity of epiphytic orchids at Gunung Manyutan Forest Reserve, a natural forest area in Wilis Mountain. Purposive sampling on 1 hectare (50 x 200 m2) eight subplots (25 x 50 m2) All data on orchid species were recorded including its number, host trees and zone of the host tree where the orchid attached. The results showed that there were 29 epiphytic orchid species recorded. Flickingeria angulata was the most abundant species (Relative Abundance of orchids/ %Fo = 38.74), continued by Appendicula sp. (%Fo = 10.91) and Eria hyacinthoides (%Fo=6.57). The three most important host trees were Pinus merkusii, Schima wallichii and Engelhardia spicata. Zone 3 (bottom part of the branches) was revealed as the most favorable part at the host tree (281 individuals), while Zone 1 (bottom part of the main stem) was the least preferable one.

Inventory and habitat study of Dendrobium capra J.J.Smith in Madiun and Bojonegoro

Abstract: Dendrobium capra J.J. Smith or “anggrek larat hijau” is the orchid of low land area which includes endangered orchid species, so it needs to conserve ex situ. The success of ex situ plant conservation influence environmental factor. That’s why habitat study of D. capra needs to be done to know about how the orchid can grow well. The observation was done in Perhutani’s teak forest between Madiun and Bojonegoro, East Java which be done in three RPH is RPH Klangon (Pajaran village, Madiun), RPH Sukun (Sambongrejo village, Bojonegoro) and RPH Gondang (Bethet village, Bojonegoro). The aims of this research are for doing study habitat and knowing the number of population on wild. The result show that D. capra can found in teak and bungur trees at 5th zone because it needs to get a direct sunshine. Some environment factors are temperature of 30-33ÂoC, humidity of 40-60% and no roots moss covered. The result of inventory showed that the numbers of D. capra on areas are 45 individual in RPH Klangon, 145 individual RPH Sukun and 58 individual RPH Gondang.© 2008 Jurusan Biologi FMIPA UNS Surakarta.Key words: Dendrobium capra J.J.Smith, habitat, Madiun, Bojonegoro.

Paraphalaenopsis Laycockii (M. R. Henderson) a. D. Hawkes: Tinjauan Terhadap Morfologi Tanaman Dan Anatomi Daun

Abstract: Porophaloenopsis laycockii is an epiphytic orchid, endemic to Borneo The orchid is not widely known since this orchid is rare and the trade of this species is restricted by the law An observation was made to provide information on plant morphology and leaf anatomy of the species Among various characters recorded, it was noted that the pencil-or-rat-tail-like leaf is about 566 mm in diameter and up to 1 m in length It has relatively large stomata measuring 668 x 573 pm with low stomata density of 163 stomata per mm ° of leaf surface area These characters may influence its water management so that the plant should be grown and maintained in shade for better establishment

The endemic orchids of Mexico: a review

Abstract: Orchidaceae is the most extensive family of flowering plants. There are approximately 30,000 - 35,000 species, belonging to 850 genera, dispersed in almost all the ecosystems on earth, except in polar zones and extreme deserts. In Mexico, there are more than 1,200 species distribuited in approximately 170 genera, of which 40% are endemic to the country. In this review, aspects related to geographical distribution, ecology, conservation, biotechnological applications and uses of orchids growing in Mexico are discussed. The geographical zones where their ecological niches are located are described and the zones that belong to priority conservation areas in Mexico are highlighted. In addition, all works on Mexican orchids related to micropropagation, medicinal uses, beneficial applications for humans, and microorganism-host interaction are discussed. In summary, this study provides perspectives for new studies and research focused on the use, propagation and conservation of Mexican endemic orchids.

A leafless epiphytic orchid, Taeniophyllum glandulosum Blume (Orchidaceae), is specifically associated with the Ceratobasidiaceae family of basidiomycetous fungi.

Abstract: Leafless epiphytes in the Orchidaceae undergo a morphological metamorphosis in which the root has chloroplast-containing cortical cells and is the sole photosynthetic organ for carbon gain. All orchids are entirely dependent on mycorrhizal fungi for their carbon supply during seed germination, and this mycorrhizal association generally persists in adult plants. However, our knowledge of the mycorrhizal association of leafless epiphytic orchids remains limited, and the contribution of the mycorrhizal association to nutrient acquisition in these orchid species is largely unknown. In this study, the mycorrhizal fungi of a leafless epiphytic orchid, Taeniophyllum glandulosum, were identified molecularly using 68 mature plants and 17 seedlings. In total, 187 fungal internal transcribed spacer sequences were obtained, of which 99% were identified as Ceratobasidiaceae. These sequences were classified into five operational taxonomic units (OTUs) based on 97% sequence similarity. The most frequent sequence was OTU1, which accounted for 91% of all Ceratobasidiaceae sequences, although other phylogenetically distinct Ceratobasidiaceae fungi were detected. These results show that T. glandulosum is specifically associated with a particular group of Ceratobasidiaceae. All mycorrhizal fungi found in T. glandulosum seedlings belonged to OTU1, which was also found in adult plants on the same host tree. The mycorrhizal fungi from 13 host tree species were compared, and T. glandulosum was preferentially associated with OTU1 on 11 tree species. In conclusion, T. glandulosum is specifically associated with Ceratobasidiaceae fungi and this specific association remains throughout the orchid life cycle and is found on divergent host tree species.

Species diversity of Selaginella in Mount Lawu, Java, Indonesia

Abstract: Setyawan AD, Sutarno, Sugiyarto. 2013. Species diversity of Selaginella in Mount Lawu, Java, Indonesia. Biodiversitas 14: 1-9. Selaginella is a genus of ferns allies that lives in moist areas and requires water for fertilization; therefore it is often found in highlands. The aim of this research was to know species diversity of Selaginella in Mount Lawu and the vicinity areas. The research was conducted between July 2007 and November 2012 on the western and southern slopes of Mount Lawu, Central- and East-Java, Indonesia, with altitudes between 1100 and 2100 m a.s.l. The research included three sites and the vicinity areas, i.e. (i) Protected forest of Cemorosewu, (ii) Grojogansewu Natural Recreation Park, and (iii) KGPAA Mangkunagoro I (Ngargoyoso) Grand Forest Park. The research found nine selaginellas species, namely: S. aristata, S. ciliaris, S. involvens, S. opaca, S. ornata, S. plana, S. remotifolia, S. singalanensis and S. zollingeriana.

Diversity of epiphytic orchids and host trees (phorophytes) in secondary forest of coban trisula, malang regency, east java, indonesia

Abstract: Introduction: Epiphytic orchids are an integral component of forest ecosystems that contributes to a high proportion of plant diversity. Host trees are a natural habitat requirement of epiphytic orchids on which the orchids live on. The aim of this study was to investigate the diversity of epiphytic orchid and the host tree in a secondary forest of Coban Trisula (the Trisula waterfall) in a Mount Bromo area. Two line transects were used, with each line transect of 150 m x 10 m was divided into 15 plots (the size of each plot 10 m x 10 m), resulting in the total number of 30 sampling plots at the study site. Data (the species name and number of individuals of epiphytic orchid, the species name and number of individuals of host tree species, vertical distribution of the epiphytic orchids on the host tree, and environmental factors (temperature and relative humidity) were recorded in each plot. Results: There were 15 epiphytic orchid species, 13 genera in the secondary forest of Coban Trisula. Appendicula angustifolia was the most abundant epiphytic orchid species (Relative abundance of orchids; % Fo=52,4%), followed by Trichotosia annulata (% Fo=29,9%). There were nine host tree species on which the orchids attached. Number of host tree species of each orchid species varied, from one to five host tree species. Trichotosia annulata was an orchid that had the highest number of host tree species; 5 species). Castanopsis javanica, was a host tree on which the highest number of individual orchids attached (Ji/Jt= 589,5), followed by Engelhardia spicata (Ji/Jt=425,67). The greatest abundance of epiphytic orchids was on zone 3 and zone 4. Mean temperature was 22,2⁰C, and relative humidity was 72,8 % at the site. Conclusion : The secondary forest of Coban Trisula had a diverse epiphytic orchid species and the host tree species. Management of orchid conservation is required to protect the diversity of orchids and the host tree species in the secondary forest in Coban Trisula from the potential human disturbances as Coban Trisula is one of tourist destinations. Key words: diversity, epiphytic orchid, host tree, secondary forest, Coban Trisula

Comparative leaf and root anatomy of two Dendrobium species (Orchidaceae) from different habitat in relation to their potential adaptation to drought

Abstract: Dendrobium capra and Dendrobium arcuatum are closely related in phylogeny, but they have very contrasting vegetative morphology and habitats. D. capra is known as a species that is well-adapted to dry lowland teak forest habitat in East Java, where most trees drop their leaves in summer, while D. arcuatum has adapted to mid or high land moist forest at elevation up to 800 m dpl. In order to investigate their potential adaptation to drought stress in the climate change era, we have compared and analyzed the leaf and root anatomical characteristics of both species. Transversal sections were made using hand mini microtome, dehydrated in graded alcohol series and stained with safranin 1 % and fastgreen 1 %. Leaf scraping technique has been used to prepare paradermal sections, and then dehydrated in graded alcohol series and stained with safranin 1 %. Quantitative anatomical characteristics between D. capra and D. arcuatum have been compared using a t-test. The result showed that there were significant differences on anatomical characters between both species. Compared to D. arcuatum, D. capra shows more developed anatomical features for adapting to drought and dry condition. These anatomical features were a thicker cuticle, thicker epidermis, presence of hypodermis, thicker mesophyll, broader primary vascular bundle, well developed xylem’s sclerenchyma, lower stomatal density, thicker and high proportion of velamen.