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Ning Wang

Bio: Ning Wang is an academic researcher from Chinese Academy of Sciences. The author has contributed to research in topics: Gene & Genomic library. The author has an hindex of 1, co-authored 2 publications receiving 3 citations.

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TL;DR: Although the ribosomal protein operons are highly conserved within domain, there are fine variations in some operons across different organisms within each domain, and these variations are informative on the evolutionary relations among the organisms.
Abstract: The organization of ribosomal proteins in 16 prokaryotic genomes was studied as an example of comparative genome analyses of gene systems. Hypothetical ribosomal protein-containing operons were constructed. These operons also contained putative genes and other non-ribosomal genes. The correspondences among these genes across different organisms were clarified by sequence homology computations. In this way a cross tabulation of 70 ribosomal proteins genes was constructed. On average, these were organized into 9–14 operons in each genome. There were also 25 non-ribosomal or putative genes in these mainly ribosomal protein operons. Hence the table contains 95 genes in total. It was found that: (i) the conservation of the block of about 20 r-proteins in the L3 and L4 operons across almost the entire eubacteria and archaebacteria is remarkable; (ii) some operons only belong to eubacteria or archaebacteria; (iii) although the ribosomal protein operons are highly conserved within domain, there are fine variations in some operons across different organisms within each domain, and these variations are informative on the evolutionary relations among the organisms. This method provides a new potential for studying the origin and evolution of old species.

2 citations

Journal ArticleDOI
TL;DR: RgMADS1 is likely a member of rice MADS box gene family and may be involved in the floral establishment and function control in the rice developmental process.
Abstract: A putative MADS box gene (RgMADS1) was cloned by screening a rice genomic library with a heterogeneous MADS box probe derived fromAntirrhinum majus squamosa gene. Sequencing and Southern analysis showed that RgMADS1 contains a highly conserved MADS box sequence, - whereas the rice genome contains numbus of MADS box genes. A chimera gene of RgMADS1 under the control of CaMV 35S promoter was wnstructed and used to transformArabidopsis. Some transformants exhibited abnormal phenotypes in their flowers, which were reflected in the floral structure, number and setting position. Therefore, RgMADS1 is likely a member of rice MADS box gene family and may be involved in the floral establishment and function control in the rice developmental process.

1 citations


Cited by
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TL;DR: A statistical methodology that largely improves the accuracy in computational predictions of transcription factor (TF) binding sites in eukaryote genomes and is coupled with any motif-finding algorithm that searches for overrepresented sequence motifs in individual species and can increase the accuracy of the coupled motif- finding algorithm.
Abstract: We present a statistical methodology that largely improves the accuracy in computational predictions of transcription factor (TF) binding sites in eukaryote genomes. This method models the cross-species conservation of binding sites without relying on accurate sequence alignment. It can be coupled with any motif-finding algorithm that searches for overrepresented sequence motifs in individual species and can increase the accuracy of the coupled motif-finding algorithm. Because this method is capable of accurately detecting TF binding sites, it also enhances our ability to predict the cis-regulatory modules. We applied this method on the published chromatin immunoprecipitation (ChIP)-chip data in Saccharomyces cerevisiae and found that its sensitivity and specificity are 9% and 14% higher than those of two recent methods. We also recovered almost all of the previously verified TF binding sites and made predictions on the cis-regulatory elements that govern the tight regulation of ribosomal protein genes in 13 eukaryote species (2 plants, 4 yeasts, 2 worms, 2 insects, and 3 mammals). These results give insights to the transcriptional regulation in eukaryotic organisms.

53 citations

Journal ArticleDOI
TL;DR: With the combination of genotype and litter size of sows, it was demonstrated that FSHβ locus is closely associated with major gene controlling litter size in commercial pig breeds, such as Yorkshire, Landrace, Durco.
Abstract: An insertion fragment in porcine FSHbeta subunit gene was cloned by PCR. Sequencing data show that the insertion is a retroposon of 292 bp sitting in intron I at the site between + 809 and +810 base. Based on these results, a PCR programme was created to genotype animal individuals in different pig breeds at FSW locus and polymorphism of FSHbeta gene was analyzed. With the combination of genotype and litter size of sows, it was demonstrated that FSHbeta locus is closely associated with major gene controlling litter size in commercial pig breeds, such as Yorkshire, Landrace, Durco. Averagely the AA sows give more 1.5 piglets than BB sows do per litter.

34 citations

Journal ArticleDOI
TL;DR: Cytoplasmic ribosomal protein complement of rice genome corresponding to 68 families is catalogued and unigene sequences corresponding to most of the reported r-proteins were identified indicating these genes are being expressed.
Abstract: Completion of rice genome sequencing has necessitated identification of transcripts encoded by the genome and their corresponding functions. In the present study we have catalogued cytoplasmic ribosomal protein complement of rice genome corresponding to 68 families. Mining TIGR (The Institute for Genomic Research) rice database and unigene sequences available from National Centre for Biotechnology Information (NCBI) produced a non-redundant set of these sequences. This resulted in identification of 209 candidate r-proteins of which 22 have not been reported previously. The number of genes per family ranges from 1 to 8 distributed throughout the genome with maximum occurrence at chromosome 7. Mapping of r-proteins on BAC clones revealed several small clusters of genes. Unigene sequences corresponding to most of the reported r-proteins were identified indicating these genes are being expressed.