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Author

Nintya Setiari

Other affiliations: Gadjah Mada University
Bio: Nintya Setiari is an academic researcher from Diponegoro University. The author has contributed to research in topics: Phalaenopsis & Chlorophyll. The author has an hindex of 7, co-authored 25 publications receiving 135 citations. Previous affiliations of Nintya Setiari include Gadjah Mada University.

Papers
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01 Jul 2009
TL;DR: The result showed that in order to get good growth on long bean, the watering volume should be half of the capacity of plant media, and high content of chlorophyll is achieved when watering volume is equal to the capacityof plant media.
Abstract: Chlorophyll is a pigment presents in leaves which is vital for photosynthesis. It absorbs red, blue and purple, and reflects green light of electromagnetic spectrum. Beside its use in photosynthesis, chlorophyll is also important in treatment for cancer, useful as disinfectant, antibiotics and food supplement. Since chlorophyll plays important role in human life, it is essential to increase chlorophyll content. One way to do that is by adjusting the watering volume based on the plant type. The aim of this research is to study the effect of different watering volume towards the growth and content of chlorophyll in long bean (Vigna sinensis). The experiment was performed by using Complete Randomized Design (3 treatments with 3 repetitions for each treatment). Data was analysed by using Anova test. The result that Anova test shows the real effect then it is followed by Duncan Multiple Range Test. The result showed that in order to get good growth on long bean, the watering volume should be half of the capacity of plant media. On the other hand, high content of chlorophyll is achieved when watering volume is equal to the capacity of plant media.

40 citations

01 Jun 2009
TL;DR: The result showed that the highest content of chlorophyll-a, b, and total found in papaya’s leaves, however the lowest one was found in sweet basil, suggesting that papaya's leave could be a food supplement.
Abstract: Chlorophyll is plant pigment which consumed as food supplement Chlorophyll source consumed now is from alfalfa’s leaves besides algae such as Spirulina and Chlorella Chlorophyll content find in all kinds of plant especially vegetables so that have potent to be source of chlorophyll The aims of this research are first to explore another chlorophyll source beside Leguminosae, from vegetables such as sweet basil, spinach, water spinach, cassava leaves, pegagan, and papaya’s leaves Second, to study the vegetables potent to be food supplement The experiment has been conducted by extracting 0,1 g each leaves in 10 mL acetone 85% The extract was fitered with Whatman paper No 1, thus record the absorbance by using spektrofotometer UV Vis Statistically analysis used to analyze chlorophyll content is Analysis of Variance (ANOVA) with Randomized Complete Design (RCD) in 95% significancy If the ANOVA showed significantly different, the analysis were continued with Duncan’s test The result showed that the highest content of chlorophyll-a, b, and total found in papaya’s leaves, however the lowest one was found in sweet basil Papaya’s leave could be a food supplement

19 citations

DOI
01 Oct 2007
TL;DR: In this paper, the effect of plant growth regulators, i.e., indol butyric acid (IBA), to the root growth in stem cutting technique of P. cablin, and the correct concentration of the IBA to obtain optimum root growth.
Abstract: This paper studies the effect of plant growth regulators, i.e., indol butyric acid (IBA), to the root growth in stem cutting technique of P. cablin, and the correct concentration of the IBA to obtain optimum root growth. The research were carried out in Lab Bio Struktur dan Fungsi Tumbuhan, UNDIP, Semarang. Complete Randome Design was used as the research method in which 4 different concentrations, i.e., control 0, 25, 50 and 75 ppm, were observed with parameter of the amount of the root, the length of the root, wetted weight, dried weight, and efficiency. The results show immersing the stem into 25 ppm IBA exhibits the highest root growth efficiency.

13 citations

Journal ArticleDOI
11 Apr 2017
TL;DR: Germination and growth of orchid seeds can be accelerated by the addition of organic supplement and plant extract in culture medium and the effect of peptone and tomato extract on early stage of embryo development of Dendrobium phalaenopsis orchids was determined.
Abstract: Germination and growth of orchid seeds can be accelerated by the addition of organic supplement and plant extract in culture medium. The objective of this study was to determine the effect of peptone and tomato extract on early stage of embryo development of Dendrobium phalaenopsis orchids. Orchid seeds were sown on NP and VW medium with addition of 10% of CW (NPCW and VWCW). Five weeks after seed germination, about 58.03% seed germination was observed on VWCW medium, and only 37.45% seed germination on NPCW. Tomato extract and peptone were added in VWCW, resulting VWCWTP medium. After 4-8 weeks on VWCWTP, 94.42% seeds was germinated into plantlet, but only 67.30% germinated seeds on VWCW. To get optimal growth and development of D . phalaenopsis orchids embryos in the in vitro condition, supplement of 100 ml.L -1 coconut water, 100 mg.L -1 tomato extract and 2 mg.L -1 peptone into VW basic medium is required.

12 citations

DOI
01 Jan 2010
TL;DR: The result showed that combination of BAP and NAA was able to induce callogenesis from hipocotyl of alfalfa and the optimal callus was obtained in combination of NAA 2 ppm and BAP 0 ppm.
Abstract: Alfalfa (Medicago sativa L.) is useful plant for treatment some diseases such as: cancer, diabetes, lupus, and hepatitis. Propagation of this plant in Indonesia face a problem which has no embryo. One method to propagate this plant is by tissue culture or micropropagation. Callus induction is first step in micropropagation to produce callus which will be regenerated to become planlet. The aims of this research are to induce callogenesis from hipocotyl of alfalfa with addition Benzyl Amino Purine (BAP) and α Naphtalene Acetic Acid (NAA), and to determine the proper combination of BAP and NAA to produce the optimal callus. The experiment has been conducted by using 12 combination of BAP and NAA with Completely Randomized Design (CRD) in 4x3 factorial pattern by 5 replicates. Data were analyzed by ANOVA 95% Degrees of Freedom (DF). If there was significance result, it was followed by DMRT analyzed at 95 % DF. The result showed that combination of BAP and NAA was able to induce callogenesis from hipocotyl of alfalfa. The optimal callus was obtained in combination of BAP 0 ppm and NAA 2 ppm.

12 citations


Cited by
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Journal ArticleDOI
TL;DR: Recent studies on Phalaenopsis equestris genome sequencing have enabled more detailed molecular studies and the molecular characterization of plantlets obtained from this technique currently allow the technique to be evaluated in a more comprehensive way regarding its real applications and main limitations aiming at mass propagation.
Abstract: The process through induction, proliferation and regeneration of protocorm-like bodies (PLBs) is one of the most advantageous methods for mass propagation of orchids which applied to the world floricultural market. In addition, this method has been used as a tool to identify genes of interest associated with the production of PLBs, and also in breeding techniques that use biotechnology to produce new cultivars, such as to obtain transgenic plants. Most of the molecular studies developed have used model plants as species of Phalaenopsis, and interestingly, despite similarities to somatic embryogenesis, some molecular differences do not yet allow to characterize that PLB induction is in fact a type of somatic embryogenesis. Despite the importance of species for conservation and collection purposes, the flower market is supported by hybrid cultivars, usually polyploid, which makes more detailed molecular evaluations difficult. Studies on the effect of plant growth regulators on induction, proliferation, and regeneration of PLBs are the most numerous. However, studies of other factors and new technologies affecting PLB production such as the use of temporary immersion bioreactors and the use of lighting-emitting diodes have emerged as new tools for advancing the technique with increasing PLB production efficiency. In addition, recent studies on Phalaenopsis equestris genome sequencing have enabled more detailed molecular studies and the molecular characterization of plantlets obtained from this technique currently allow the technique to be evaluated in a more comprehensive way regarding its real applications and main limitations aiming at mass propagation, such as somaclonal variation.

61 citations

Journal ArticleDOI
TL;DR: The result showed that Kuwait accession cultivated at middle altitude contains the highest levels of thymoquinone (2940.43 mg/kg), and the highest Levels of Thymol were found in India accessions cultivated at high altitude (141.46 mg /kg).
Abstract: Black cumin cultivated in many subtropical regions in the world, including Asia, Middle East, and North Africa. The most active constituent of black cumin is thymoquinone representing 18.4%–24% of the volatile oil and thymol. Data about thymoquinone and thymol came from the country of origin, but no data from tropical region. This study aimed to analyze the production of chlorophyll, thymoquinone, thymol, and malondialdehyde from black cumin cultivated at three altitudes of Indonesian tropical region. The result showed that Kuwait accession cultivated at middle altitude contains the highest levels of thymoquinone (2940.43 mg/kg), and the highest levels of thymol were found in India accession cultivated at high altitude (141.46 mg/kg). Data showed that the level of malondialdehyde at low (220 meter above sea level [masl]) and middle (560 masl) altitudes is higher than high (1.280 masl) altitude.

24 citations

Dissertation
20 Sep 2019
TL;DR: High anthocyanin content in this plant is able to be a source of antioxidants to ward off free radicals, the need for this crop is quite high, but it is not being offset by the farmers' low yields.
Abstract: INDONESIA: Bayam Merah (Alternanthera amoena Voss. var. Red Leaf) merupakan tanaman dengan banyak manfaat bagi kesehatan, tingginya kandungan antosianin pada tanaman ini, mampu menjadi sumber antioksidan untuk menangkal radikal bebas. Kebutuhan akan tanaman ini cukup tinggi, namun tidak diimbangi oleh hasil produksi petani yang tergolong rendah. Oleh karena itu, perlu dilakukan upaya pemuliaan tanaman melalui induksi poliploidi menggunakan oryzalin, untuk menghasilkan tanaman dengan jumlah kromosom yang berlipat ganda, sel yang lebih besar, karakter morfologi dan produktivitas yang lebih baik. Penggunaan oryzalin jauh lebih efektif dalam poliploidi tanaman, dengan konsentrasi rendah (µM), dapat menghasilkan tanaman poliploidi. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh lama perendaman dan kosentrasi oryzalin terhadap jumlah kromosom, stomata, karakter morfologi dan produktivitas tanaman A. amoena Voss. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) dua faktorial, faktor pertama yaitu lama perendaman oryzalin selama 4, 8 dan 24 jam, fakor kedua yaitu konsentrasi 0, 1.25, 2.5, 3.75 dan 5µM, dan dilakukan sebanyak 3 kali ulangan. Parameter yang diamati yaitu jumlah kromosom, karakter stomata, karakter morfologi dan produktivitas tanaman. Analisis data menggunkan ANOVA dengan uji lanjut Duncan 5%. Hasil penelitian menunjukkan bahwa, pemberian oryzalin mampu meningkatkan jumlah kromosom tanaman A. amoena Voss. varietas Red Leaf, yaitu menghasilkan kromosom 3n=18 (Triploid), 4n=24 (Tetraploid), 5n= 30 (Pentaploid) dan 6n= 36 (Hexaploid). Perlakuan yang paling optimal adalah lama perendaman 8 jam dengan konsentrasi 2,5 µM Mampu mempengaruhi ukuran stomata, jumlah kloroplas di sel penjaga, karakter morfologi dan produktivitas tanaman A. amoena Voss. varietas Red Leaf. ENGLISH: Red Spinach (Alternanthera amoena Voss. Var. Red Leaf) is a plant with a ton of health benefits, high anthocyanin content in this plant is able to be a source of antioxidants to ward off free radicals. The need for this crop is quite high, but it is not being offset by the farmers' low yields. Therefore, efforts should be made to plant breeding through induction of polyploidy using oryzalin, to produce plants with multiple chromosomes, larger cells, morphological characters and better productivity. The use of oryzalin is far more effective in plant polyploidy, because with only low concentrations (µM), it is already able to produce polyploidy plants. The purpose of this study was to determine the effect of the interaction of immersion duration and oryzalin concentration on increasing the genetic diversity of A. amoena Voss plants. This research method uses two factorial Randomized Block Design (RBD), which has the length immersion of oryzalin of 4, 8 and 24 hours with concentrations of 0, 1.25, 2.5, 3.75 and 5µM, and carried out 3 times. The results obtained were analysed using analysis of variance (ANOVA) with Duncan's further test 5%. The results showed that, administration of oryzalin can increase the number of A. amoena Voss chromosomes. Red Leaf varieties, which produce chromosomes 3n = 18 (Triploid), 4n = 24 (Tetraploid), 5n = 30 (Pentaploid) and 6n = 36 (Hexaploid). The most optimal treatment is immersion time of 8 hours with a concentration of 2.5 µM able to affect the size of the stomata, the amount of chloroplasts in guard cells, morphological characters and productivity of A. amoena Voss plants. Red Leaf varieties.

19 citations

Journal ArticleDOI
TL;DR: The use of chlorophyll extract of papaya leaf as an exogenous photosensitizer to kill the Candida albicans biofilms after being activated by the laser resulted in the maximum spectrum of absorption at 414 nm and 668 nm, which produced a maximum reduction effect after photoinactivation.
Abstract: Introduction: Photodynamic inactivation has been developed to kill pathogenic microbes. In addition, some techniques have been introduced to minimize the biofilm resistance to antifungal properties in inhibiting cell growth. The principle of photodynamic inactivation different to antifungal drugs therapy which is resistant to biofilms. The presence of reactive oxygen species (ROS) that generating in photodynamic inactivation mechanisms can be damaging of biofilm cells and the principle of light transmission that could be penetrating in matrix layers of extracellular polymeric substance (EPS) until reaching the target cells at the base layers of biofilm. The present work aims to explore the potential of chlorophyll extract of papaya leaf as an exogenous photosensitizer to kill the Candida albicans biofilms after being activated by the laser. The potential of chlorophyll photosensitizer was evaluated based on the efficacy of inactivation C. albicans biofilm cell through a cell viability test and an organic compound test. Methods: The treatment of photoinactivation was administered to 12 groups of C. albicans biofilm for four days using the 445 nm laser and the 650 nm laser. The 445 nm and 650 nm lasers activated the chlorophyll extract of the papaya leaf (0.5 mg/L) at the same energy density. The energy density variation was determined as 5, 10, 20, 30 and 40 J/cm2 with the duration of exposure of each laser adjusted to the absorbance percentage of chlorophyll extract of the papaya leaf. Results: The absorbance percentage of chlorophyll extracts of the papaya leaf on wavelengths of 650 nm and 445 nm respectively were 22.26% and 60.29%, respectively. The most effective treated group was a group of the laser with the addition of chlorophyll, done by the 650 nm lasers with inactivation about 32% (P=0.001), while the 445 nm lasers only 25% (P=0.061). The maximum malondialdehyde levels by treatment of the laser 650 nm were (0.046±0.004) nmol/mg. Conclusion: The use of chlorophyll extract of the papaya leaf as a photosensitizer, resulted in the maximum spectrum of absorption at 414 nm and 668 nm, which produced a maximum reduction effect after photoinactivation up to 32% (with chlorophyll) and 25% (without chlorophyll). The utilization of chlorophyll extract of the papaya leaf would increase the antifungal effects with the activation by the diode laser in the biofilm of C. albicans.

15 citations

Journal ArticleDOI
01 Nov 2019
TL;DR: In this article, the formulation and dosage of biological fertilizers consortium of cellulolytic bacteria based on organic liquid waste that was appropriate for the growth and yield of red pepper plants were investigated.
Abstract: Red pepper production in 2016-2017 was increased. Although production has increased, demand for pepper will continue to increase, and efforts to increase soil fertility still need to be sought. To maintain and increase soil productivity, a combination of inorganic fertilizers with appropriate organic fertilizers is needed. One of the organic fertilizers is biofertilizers. This research aimed at finding out the formulation and dosage of biological fertilizers consortium of cellulolytic bacteria based on organic liquid waste that was appropriate for the growth and yield of red pepper plants. This research method used a Completely Randomized Design with 12 treatments, namely (5, 10, 15) ml of biological fertilizer of waste water washing rice, (5, 10, 15) ml of biological fertilizer of coconut water waste, (5, 10, 15) ml of biological fertilizer of tofu water waste, (5, 10, 15) ml of palm oil liquid fertilizer. The results showed that all biological fertilizer application based on organic liquid waste cellulolytic was able to give varied results on each parameter observed so that all wastes could be applied to pepper plants. Tofu wastewater biofertilizer with a dose of 10 ml give a good response to the production of pepper plants compared to other treatments.

13 citations