Ofer Shai

TL;DR: It is estimated that transcripts from ∼95% of multiexon genes undergoAlternative splicing and that there are ∼100,000 intermediate- to high-abundance alternative splicing events in major human tissues.

TL;DR: The assembly of a ‘splicing code’ is described, which uses combinations of hundreds of RNA features to predict tissue-dependent changes in alternative splicing for thousands of exons and facilitates the discovery and detailed characterization of regulatedAlternative splicing events on a genome-wide scale.

TL;DR: Interestingly, it is found that transcription and AS act independently on different sets of genes in order to define tissue-specific expression profiles, which reflect tissue identity.

TL;DR: It is concluded that 'functional genomics' strategies based on quantitative transcriptional co-expression will be as fruitful in mammals as they have been in simpler organisms, and that transcriptional control of mammalian physiology is more modular than is generally appreciated.

TL;DR: Using AS microarrays, it is shown that PTC-containing splice variants are generally produced at uniformly low levels across diverse mammalian cells and tissues, independently of the action of NMD.