Author
Olawale Arogundade
Bio: Olawale Arogundade is an academic researcher from University of Ilorin. The author has contributed to research in topics: Cucumber mosaic virus & Pepper. The author has an hindex of 3, co-authored 14 publications receiving 67 citations.
Topics: Cucumber mosaic virus, Pepper, Virus, Mosaic virus, Biology
Papers
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TL;DR: In ELISA test some of the samples from the pepper farms showed positive reaction to single infection with PVMV, CMV, while some others showedpositive reaction to mixed infection of the two viruses but some also negative reaction to P VMV and CMV antisera.
Abstract: Viral diseases constitute obstacles to pepper production in the world. In Nigeria, pepper plants are primarily affected by pepper veinal mottle virus (PVMV), Cucumber mosaic virus (CMV), Pepper leaf curl Virus (TLCV), Tobacco mosaic virus (TMV), Pepper mottle virus (PMV) and a host of other viruses. The experiment was carried out with a diagnostic survey on the experimental field of the National Horticultural Research Institute, Ibadan, Nigeria and on pepper farms in six local government areas within Ibadan Oyo State, Nigeria, forty samples were collected from each of the farms. Diseased samples were obtained from the field and taken to the laboratory for indexing. In ELISA test some of the samples from the pepper farms showed positive reaction to single infection with PVMV (36.79%), CMV (22.14%) while some others showed positive reaction to mixed infection of the two viruses (10%) but some also negative reaction to PVMV and CMV antisera (31.07).
38 citations
TL;DR: The results of the experiment revealed that all soybean cultivars were susceptible to single and mixed infection of the two viruses but to seemingly different extent, indicating that there was little or no synergistic effect between the two virus on soybean.
Abstract: The study was carried out to determine the comparative pathogenic response of six cultivars of soybean; TGx 1844-18E, TGx 1448-2E, TGx 1910-8F, TGx 1019-2EN, TGx 1910-8F and TGx 1876-4E to single and mixed infections with cowpea mottle virus and cucumber mosaic virus. The experiment was conducted in the screenhouse at the crop production pavilion, Faculty of Agriculture, University of Ilorin, Ilorin, Kwara state Nigeria. The results of the experiment revealed that all soybean cultivars were susceptible to single and mixed infection of the two viruses but to seemingly different extent. The single infection with cowpea mottle virus (CMeV), however, caused the most severe symptoms on the soybean cultivars. Cucumber mosaic virus (CMV) alone was not as severe as the CMeV. The mixed infection of CMeV and CMV did not cause higher severity than CMeV alone indicating that there was little or no synergistic effect between the two viruses on soybean.
12 citations
TL;DR: The tolerance level of the legumes, as shown by the percentage loss in selected growth and yield attributes, indicated that line TGx 1910-8F is the most tolerant while TGx 1844-18E is themost susceptible to single and mixed infection of the two viruses.
Abstract: Cowpea mottle virus is of localised importance on cowpea in Nigeria infecting several leguminous species andCucumber mosaic virus is of worldwide distribution infecting several horticultural crops.A study was carried out in the screen house of the crop production pavilion, Faculty of Agriculture, University of Ilorin,Ilorin, Kwara State, Nigeria to determine the pathogenic response of six lines of soybean (TGx 1844-18E, TGx 1448-2E,TGx- 1910-8F, TGx 1019-2EN, TGx 1844-4E and TGx 1876-4E) to single and mixed infection with cowpea mottlevirus (CMeV) and cucumber mosaic virus (CMV).The Lines obtained from National Cereal Research Institute, Badeggiwere used for the experiment.Two viable Seeds were sown per 4litre plastic pots filled with sterilized soil; inoculation of viral isolates was carried outmechanically on the soybean at two weeks after planting.The results revealed that all Soybean lines tested were susceptible to single and mixed infection of the two viruses.However the tolerance level of the legumes, as shown by the percentage loss in selected growth and yield attributesindicated that line TGx 1910-8F is the most tolerant while TGx 1844-18E is the most susceptible to single and mixedinfection.Hence, TGx 1910-8F is most suitable in areas where there is an outbreak of disease caused by CMeV and CMV.
11 citations
TL;DR: The ability to be transmitted by seed, and the rate of seed transmission of CMV, is cultivar dependent, and a reverse transcription polymerase chain reaction assay was performed for comparison.
Abstract: Cucumber mosaic virus (CMV) occurs worldwide and is a harmful pathogen for pepper (Capsicum spp.). Transmission of the virus is normally by insect. The possibility of transmission by seed is less c...
10 citations
TL;DR: The results of the morphological and micro-satellite (SSR) analyses showed wide diversity among the mango cultivar used in this study as discussed by the authors , indicating high level of variability and the presence of outbreeding.
Abstract: ABSTRACT Morphological and microsatellites (SSR) markers are efficient tools for determining genetic relatedness among mango cultivars. Seventeen mango cultivars were used for this study. Eight fruit quantitative traits were collected and subjected to mean separation using One-Way ANOVA and correlation using Principal Component Analysis (PCA). Also, molecular analysis was done using PCR-based SSR markers. The resulting binary matrix was analyzed using Numerical Taxonomy and Multivariate Analysis System (NTSYSpc). Significant variation (p ≤ .05) among all the 17 mango cultivars was observed for the eight quantitative traits studied. The PCA showed that the fruits length, width, thickness weights, %pulp and %stone contributed to 98.73% of the variation observed in all the mango cultivars. A total of 21 alleles were detected from the seven polymorphic primers ranging from two to five alleles per locus with an average of 3.0 alleles per locus. The polymorphic information content (PIC) ranged from 0.52 to 0.80 with an average of 0.66. Both the morphological and molecular markers showed that the mango cultivars were diverse except for ‘Saigon’ and ‘Julie’ as well as ‘Harden’ and ‘Lipen’ which though appear morphologically distinct based on the understudied traits but showed strong similarity to each other through molecular analysis. Dendrogram constructed using the Unweighted Pair Group Method with Arithmetic mean (UPGMA) based on SSR markers revealed a similarity coefficient of 48–93% indicating high level of variability and the presence of outbreeding. Results of the morphological and microsatellite (SSR) analyses showed wide diversity among the mango cultivar used in this study.
4 citations
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TL;DR: This review gives an account of the loss in production and yield procured in chili cultivation due to anthracnose disease in Indian sub-continent, with emphasis given to the sustainable management strategies against the conventionally recommended control for the disease.
Abstract: Indian cuisine is renowned and celebrated throughout the world for its spicy treat to the tongue. The flavor and aroma of the food generated due to the use of spices creates an indelible experience. Among the commonly utilized spices to stimulate the taste buds in Indian food, whole or powdered chilli constitutes an inevitable position. Besides being a vital ingredient of of Indian food, chilli occupy an important position as an economic commodity, a major share in Indian economy. Chilli also has uncountable benefits to human health. Fresh green chilli fruits contain more Vitamin C than found in citrus fruits, while red chilli fruits have more Vitamin A content than as found in carrots. The active component of the spice, Capsaicin possesses the antioxidant, anti-mutagenic, anti-carcinogenic and immunosuppressive activities having ability to inhibit bacterial growth and platelet aggregation. Though introduced by the Portuguese in the Seventeenth century, India has been one of the major producers and exporters of this crop. During 2010-2011, India was the leading exporter and producer of chilli in the world, but recently due to a decline in chilli production, it stands at third position in terms of its production. The decline in chilli production has been attributed to the diseases linked with crop like anthracnose or fruit rot causing the major share of crop loss. The disease causes severe damage to both mature fruits in the field as well as during their storage under favorable conditions, which amplifies the loss in yield and overall production of the crop. This review gives an account of the loss in production and yield procured in chili cultivation due to anthracnose disease in Indian sub-continent, with emphasis given to the sustainable management strategies against the conventionally recommended control for the disease. Also, the review highlights the various pathogenic species of Colletotrichum spp, the causal agent of the disease, associated with the host crop in the country. The information in the review will prove of immense importance for the groups targeting the problem, for giving a collective information on various aspects of the epidemiology and management of the disease.
124 citations
TL;DR: In ELISA test some of the samples from the pepper farms showed positive reaction to single infection with PVMV, CMV, while some others showedpositive reaction to mixed infection of the two viruses but some also negative reaction to P VMV and CMV antisera.
Abstract: Viral diseases constitute obstacles to pepper production in the world. In Nigeria, pepper plants are primarily affected by pepper veinal mottle virus (PVMV), Cucumber mosaic virus (CMV), Pepper leaf curl Virus (TLCV), Tobacco mosaic virus (TMV), Pepper mottle virus (PMV) and a host of other viruses. The experiment was carried out with a diagnostic survey on the experimental field of the National Horticultural Research Institute, Ibadan, Nigeria and on pepper farms in six local government areas within Ibadan Oyo State, Nigeria, forty samples were collected from each of the farms. Diseased samples were obtained from the field and taken to the laboratory for indexing. In ELISA test some of the samples from the pepper farms showed positive reaction to single infection with PVMV (36.79%), CMV (22.14%) while some others showed positive reaction to mixed infection of the two viruses (10%) but some also negative reaction to PVMV and CMV antisera (31.07).
38 citations
TL;DR: Mixed infections were common in the farmers' fields and high incidence suggests the cultivars are highly susceptible to viral infections.
Abstract: Surveys to determine the incidence, diversity and distribution of viruses infecting pepper ( Capsicum spp.) were conducted in six states (Oyo, Ondo, Osun, Ogun, Ekiti and Lagos) of South-west Nigeria in 2010 and 2011. Leaf samples from symptomatic and asymptomatic plants were collected at random from farmers’ fields and were analyzed for viruses using antigen coated plate enzyme-linked immunosorbent assay (ACP-ELISA). Symptoms observed on infected plants included mosaic, mottling, puckering, reduction in leaf size, vein yellowing, leaf and fruit deformation and stunting. The average disease incidence was 79% in 2010 and 76% in 2011; the average disease severity score was 2.9 in both years. This study identified eight viruses in the leaf samples as follows: Potato virus Y (PVY), Potato virus X (PVX), Pepper veinal mottle virus (PVMV), Pepper mild mottle virus (PMMV), Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV), Tobacco etch virus (TEV) and Tomato mosaic virus (ToMV). Incidence of PVY was the highest (79%), followed by TEV (67%), CMV (61%), and PVMV (58%); lowest in ToMV (23%). Mixed infections were common in the farmers' fields and high incidence suggests the cultivars are highly susceptible to viral infections. Keywords: Pepper viruses, Capsicum spp., virus survey, enzyme linked immunosorbent assay (ELISA), Nigeria
25 citations
Dissertation•
01 Jan 2013
TL;DR: In this article, the authors propose a method to solve the problem of concatenation: 1.169, 1.18, 2.0, 3.1, 4.
Abstract: 169
14 citations
01 Jan 1989
11 citations