O
Olivier Bensaude
Researcher at École Normale Supérieure
Publications - 70
Citations - 5229
Olivier Bensaude is an academic researcher from École Normale Supérieure. The author has contributed to research in topics: RNA polymerase II & Transcription (biology). The author has an hindex of 34, co-authored 66 publications receiving 4925 citations. Previous affiliations of Olivier Bensaude include Centre national de la recherche scientifique & French Institute of Health and Medical Research.
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Journal ArticleDOI
7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes
TL;DR: It is reported that in human HeLa cells more than half of the P-TEFb is sequestered in larger complexes that also contain 7SK RNA, an abundant, small nuclear RNA of hitherto unknown function, which may contribute to an important feedback loop modulating the activity of RNA Pol II.
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Binding of the 7SK snRNA turns the HEXIM1 protein into a P-TEFb (CDK9/cyclin T) inhibitor
Annemieke A. Michels,Alessandro Fraldi,Qintong Li,Todd E. Adamson,François Bonnet,Van Trung Nguyen,Stanley C. Sedore,Jason P. Price,David H. Price,Luigi Lania,Olivier Bensaude +10 more
TL;DR: An in vitro reconstitution of 7SK‐dependent HEXIM1 association to purified P‐TEFb and subsequent CDK9 inhibition is reported and it is proposed that the RNA‐binding domain of HexIM1 mediates its association with 7SK and that P‐ TEFb then enters the complex through association with H EXIM1.
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Single-molecule tracking in live cells reveals distinct target-search strategies of transcription factors in the nucleus
Ignacio Izeddin,Vincent Récamier,Lana Bosanac,Lana Bosanac,Ibrahim I Cisse,Lydia Boudarene,Lydia Boudarene,Claire Dugast-Darzacq,Claire Dugast-Darzacq,Florence Proux,Olivier Bénichou,Raphaël Voituriez,Olivier Bensaude,Maxime Dahan,Maxime Dahan,Maxime Dahan,Xavier Darzacq,Xavier Darzacq +17 more
TL;DR: In this article, the authors implemented a single-molecule tracking assay to determine TFs dynamics and found that c-Myc is a global explorer of the nucleus while P-TEFb is a local explorer that oversamples its environment.
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Investigating RNA polymerase II carboxyl-terminal domain (CTD) phosphorylation
TL;DR: A panel of monoclonal antibodies is available that distinguishes between phosphorylated isoforms of RNA polymerase II's (RNAP II) largest subunit, and how global RNAP II phosphorylation changes are affected by the physiological context such as cell stress and embryonic development is discussed.
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In vivo degradation of RNA polymerase II largest subunit triggered by alpha-amanitin.
Van Trung Nguyen,Federico Giannoni,Marie-Françoise Dubois,Sook-Jae Seo,Marc Vigneron,Claude Kedinger,Olivier Bensaude +6 more
TL;DR: In murine fibroblasts exposure to alpha-amanitin triggered degradation of the RPB1 subunit, while other RNAPII subunits, RPB5 and RPB8, remained almost unaffected, in contrast to other inhibitors, such as actinomycin D or 5,6-dichloro-1-beta-D-ribofuranosyl-benzimidazole.