O
Opher Gileadi
Researcher at Structural Genomics Consortium
Publications - 159
Citations - 7214
Opher Gileadi is an academic researcher from Structural Genomics Consortium. The author has contributed to research in topics: Helicase & DNA repair. The author has an hindex of 45, co-authored 154 publications receiving 6294 citations. Previous affiliations of Opher Gileadi include University of Texas Southwestern Medical Center & Stanford University.
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Journal ArticleDOI
Protein production and purification.
Susanne Gräslund,Pär Nordlund,J Weigelt,B M Hallberg,James E. Bray,Opher Gileadi,Stefan Knapp,Udo Oppermann,Cheryl H. Arrowsmith,Raymond Hui,J Ming,Sirano Dhe-Paganon,Park H-W.,Alexei Savchenko,Adelinda Yee,Aled M. Edwards,Renaud Vincentelli,Christian Cambillau,R Kim,Kim S-H.,Zihe Rao,Yunyu Shi,Thomas C. Terwilliger,Kim C-Y.,Hung L-W.,Geoffrey S. Waldo,Yoav Peleg,Shira Albeck,Tamar Unger,Orly Dym,Jaime Prilusky,Joel L. Sussman,Raymond C. Stevens,Scott A. Lesley,Scott A. Lesley,Ian A. Wilson,Ian A. Wilson,Andrzej Joachimiak,Frank R. Collart,Irina Dementieva,Mark I. Donnelly,William H. Eschenfeldt,Youngchang Kim,Lucy Stols,Ruiying Wu,M. Zhou,Stephen K. Burley,J.S. Emtage,J.M. Sauder,D.A. Thompson,Kevin Bain,John G. Luz,Tarun Gheyi,F Zhang,Shane Atwell,Steven C. Almo,Jeffrey B. Bonanno,Andras Fiser,Soumya Swaminathan,F.W. Studier,Mark R. Chance,Andrej Sali,Thomas Acton,Rong Xiao,L Zhao,L C Ma,John F. Hunt,Liang Tong,Kellie Cunningham,Masayori Inouye,Stephen Anderson,Haleema Janjua,Ritu Shastry,C K Ho,D Wang,Hao Wang,M Jiang,Gaetano T. Montelione,David I. Stuart,Raymond J. Owens,S Daenke,Anja Schütz,Udo Heinemann,S Yokoyama,Konrad Büssow,Kristin C. Gunsalus,Kristin C. Gunsalus +86 more
TL;DR: This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
Journal ArticleDOI
Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study
TL;DR: The trend is that heterologous expression of some proteins in bacteria can be improved by altering codon preference, but that this effect can be generally recapitulated by introducing rare codon tRNAs into the host cell.
Journal ArticleDOI
Crystal structures of histone demethylase JMJD2A reveal basis for substrate specificity.
S.S. Ng,Kathryn L. Kavanagh,Michael A. McDonough,Danica Butler,Ewa S. Pilka,Benoît M. R. Liénard,James E. Bray,Pavel Savitsky,Opher Gileadi,Frank von Delft,Nathan R. Rose,John Offer,Johanna C. Scheinost,Tomasz Borowski,Michael Sundström,Christopher J. Schofield,Udo Oppermann +16 more
TL;DR: How human JMJD2A (jumonji domain containing 2A), which is selective towards tri- and dimethylated histone H3 lysyl residues 9 and 36, discriminates between methylation states and achieves sequence selectivity for H3K9 is revealed.
Journal ArticleDOI
High-throughput production of human proteins for crystallization: the SGC experience.
Pavel Savitsky,James E. Bray,Christopher D.O. Cooper,Brian D. Marsden,Pravin Mahajan,N.A. Burgess-Brown,Opher Gileadi +6 more
TL;DR: The methods utilized in the Structural Genomics Consortium in Oxford are described, resulting in successful purification of 48% of human proteins attempted; of those, the structures of ∼40% were solved by X-ray crystallography.
Journal ArticleDOI
Scanning electron microscopy of cells and tissues under fully hydrated conditions
Stephan Y. Thiberge,Amotz Nechushtan,David Sprinzak,Opher Gileadi,Vered Behar,Ory Zik,Yehuda Chowers,Shulamit Michaeli,Joseph Schlessinger,Elisha Moses +9 more
TL;DR: A hybrid technique combining the ease of use and ability to see into cells of optical microscopy with the higher resolution of electron microscopy is presented, indicating a potential for multilabeling and specific scintillating markers.