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P. Chen

Bio: P. Chen is an academic researcher. The author has contributed to research in topics: Klebsiella pneumoniae & Tyrosine phosphorylation. The author has an hindex of 1, co-authored 1 publications receiving 4 citations.

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TL;DR: The polysaccharide isolated from the capsule of Klebsiella pneumoniae serotype K24 was found to suppress the proliferation of Ehrlich ascites tumour cells in vitro, but did not alter the cell cycle distribution of cells.
Abstract: K24 capsular polysaccharide (K24-CPS), with a known structure of a repeating unit, was isolated from the capsule of Klebsiella pneumoniae serotype K24. The polysaccharide was found to suppress the proliferation of Ehrlich ascites tumour (EAT) cells in vitro, but did not alter the cell cycle distribution of cells. K24-CPS treatment reduced the tyrosine phosphorylation of some proteins in EAT cells. Furthermore, the treatment also decreased the expression of c-JUN, but had no effect on the levels of c-FOS and c-MYC. It is speculated that the growth suppression effect of K24-CPS may be related to its effect in down-regulating c-JUN expression.

4 citations


Cited by
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TL;DR: Among different exogeneous BRMs, polysaccharide BRMs have the widest occurrence in nature and have been tested for their therapeutic properties in human clinical trials.

367 citations

Journal ArticleDOI
TL;DR: Anti-tumour test in vitro showed that both complexes have properties to inhibit growth of L1210 cell lines and have similar bioactivities, but might have different mechanism.

20 citations

Journal ArticleDOI
TL;DR: Industrial wastewater from the production of sulfur containing esters and the resulting products of this synthesis, EHTG and IOTG, were deployed in this study to enrich novel bacterial strains, since no wastewater and E HTG or IotG degrading microorganisms were hitherto described or available.
Abstract: Industrial wastewater from the production of sulfur containing esters and the resulting products of this synthesis, 2-ethylhexylthioglycolic acid (EHTG) and iso-octylthioglycolic acid (IOTG), were deployed in this study to enrich novel bacterial strains, since no wastewater and EHTG or IOTG degrading microorganisms were hitherto described or available. In addition, nothing is known about the biodegradation of these thiochemicals. The effect of this specific wastewater on the growth behaviour of microorganisms was investigated using three well-known Gram-negative bacteria (Escherichia coli, Pseudomonas putida, and Ralstonia eutropha). Concentrations of 5% (v/v) wastewater in complex media completely inhibited growth of these three bacterial strains. Six bacterial strains were successfully isolated, characterized and identified by sequencing their 16S rRNA genes. Two isolates referred to as Achromobacter sp. strain MT-E3 and Pseudomonas sp. strain MT-I1 used EHTG or IOTG, respectively, as well as the wastewater as sole source of carbon and energy for weak growth. More notably, both isolates removed these sulfur containing esters in remarkable amounts from the cultures supernatant. One further isolate was referred to as Klebsiella sp. strain 58 and exhibited an unusual high tolerance against the wastewater’s toxicity without utilizing the contaminative compounds. If cultivated with gluconic acid as additional carbon source, the strain grew even in presence of more than 40% (v/v) wastewater. Three other isolates belonging to the genera Bordetella and Pseudomonas tolerated these organic sulfur compounds but showed no degradation abilities.

11 citations