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P. Ganchevska

Bio: P. Ganchevska is an academic researcher from Medical University Plovdiv. The author has contributed to research in topics: Cell morphology & Mitotic index. The author has an hindex of 1, co-authored 1 publications receiving 3 citations.

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TL;DR: Novel evidence for altered expression of proliferative antigens and enhanced expression of HBGA B in fibroblasts treated with dental monomers bis-GA and bis-GMA is presented suggesting that these substances affect cell morphology, proliferative activity, and antigenic profile.
Abstract: The present work is focused on examining the effect of the structurally similar dental monomers bis-GA and bis-GMA on the expression of histo-blood group antigens (HBGAs) in comparison with fibroblast vitality and proliferation. The fibroblast cell line McCoy-Plovdiv was cultivated in a serum-free medium and was treated with both monomers. Cell vitality was measured by the crystal violet test. Mitotic index and cell morphology were assessed. An immunocytochemical technique was applied to follow the expression of proliferative antigens PCNA and Ki-67 and of HBGA. The expression level of HBGA was measured by an improved pixel selection algorithm with proprietary software. The lowest concentration of 2.5 μmol/L did not significantly affect morphology, vitality, or proliferation activity. Interestingly, the quantitative analysis revealed augmented expression of HBGA B at 2.5 μmol/L. The higher concentrations of the dental monomers reduced cell vitality and mitotic indices and altered proliferative antigen expression. Bis-GA proved to be more toxic than bis-GMA and caused more prominent alterations including greater enhancement of HBGA B expression. We present novel evidence for altered expression of proliferative antigens and enhanced expression of HBGA B in fibroblasts treated with dental monomers bis-GA and bis-GMA suggesting that these substances affect cell morphology, proliferative activity, and antigenic profile.

3 citations


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TL;DR: This study investigated the influence of the degree of conversion (DC), resin-based composites (RBC) composition, and the effect of additional violet light from one light curing unit (LCU) on cell attachment/growth, eluate cytotoxicity, and gene expression.

36 citations

01 Jan 2016
TL;DR: Assessment of the potential of the antibiotic G-418 as a selective agent for transfection procedures of McCoy-Plovdiv cells determined its cytotoxic effect on untransfected cells and medium replacement during treatment led to higher effective antibiotic concentrations and thus to increased cell death rate.
Abstract: McCoy-Plovdiv cells represent a serum-free in vitro system, which is suitable not only for diagnostic purposes but also for studies on the biological activity of different substances. In addition, it can be used for developing an expression system for production and purification of various proteins. Isolation of successfully transfected cells is an important step in this process and different selection systems can be employed for this purpose. In order to assess the potential of the antibiotic G-418 as a selective agent for transfection procedures of McCoy-Plovdiv cells, we determined its cytotoxic effect on untransfected cells. Two commercially available versions of the antibiotic were tested: products of Sigma-Aldrich and Duchefa Biochemie. Cells were treated with a wide range of G-418 concentrations and cell vitality was measured with the WST-1 reagent after 48 and 96 hours of treatment. Our results showed that concentrations in the range 0.5-0.6 mg/ml of G-418 from Sigma-Aldrich efficiently kill untransfected McCoy-Plovdiv cells whereas G-418 from Duchefa Biochemie should be applied at higher concentrations: 0.9-1 mg/ml. Furthermore, medium replacement during treatment led to higher effective antibiotic concentrations and thus to increased cell death rate, exclusively in cultures treated with high G-418 concentrations.
Journal ArticleDOI
TL;DR: In this article, the potential of G-418 as a selective agent for transfection procedures of McCoy-Plovdiv cells was assessed and its cytotoxic effect on untransfected cells.
Abstract: McCoy-Plovdiv cells represent a serum-free in vitro system, which is suitable not only for diagnostic purposes but also for studies on the biological activity of different substances. In addition, it can be used for developing an expression system for production and purification of various proteins. Isolation of successfully transfected cells is an important step in this process and different selection systems can be employed for this purpose. In order to assess the potential of the antibiotic G-418 as a selective agent for transfection procedures of McCoy-Plovdiv cells, we determined its cytotoxic effect on untransfected cells. Two commercially available versions of the antibiotic were tested: products of Sigma-Aldrich and Duchefa Biochemie. Cells were treated with a wide range of G-418 concentrations and cell vitality was measured with the WST-1 reagent after 48 and 96 hours of treatment. Our results showed that concentrations in the range 0.5-0.6 mg/ml of G-418 from Sigma-Aldrich efficiently kill untransfected McCoy-Plovdiv cells whereas G-418 from Duchefa Biochemie should be applied at higher concentrations: 0.9-1 mg/ml. Furthermore, medium replacement during treatment led to higher effective antibiotic concentrations and thus to increased cell death rate, exclusively in cultures treated with high G-418 concentrations.