Author
P.S. Appukuttan
Other affiliations: Uniformed Services University of the Health Sciences
Bio: P.S. Appukuttan is an academic researcher from Sree Chitra Thirunal Institute for Medical Sciences and Technology. The author has contributed to research in topics: Affinity chromatography & Antibody. The author has an hindex of 12, co-authored 46 publications receiving 508 citations. Previous affiliations of P.S. Appukuttan include Uniformed Services University of the Health Sciences.
Topics: Affinity chromatography, Antibody, Lectin, Glycoprotein, Antigen
Papers
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TL;DR: An α-D-galactose-specific lectin from the seeds of jack fruit has been isolated in pure form by affinity chromatography on immobilised guar gum, indicating that the native lectin is a tetrarner of identical subunits.
Abstract: An α-D-galactose-specific lectin from the seeds of jack fruit (Artocarpus integra) has been isolated in pure form by affinity chromatography on immobilised guar gum (a galactomannan). The lectin is shown to be a glycoprotein containing 3% carbohydrate and having a molecular weight of 39,500 as determined by gel filtration. Sodium dodecyl sulphate gel electrophoresis revealed a single polypeptide of 10,500 dalton, indicating that the native lectin is a tetrarner of identical subunits. The hemagglutinating activity of the lectin towards erythrocytes of all blood groups is found to be the same.
85 citations
Journal Article•
67 citations
TL;DR: Modifications of PNA-binding glycoproteins, including selective removal of O-linked oligosaccharides and treatment with glycosidases revealed that Galbeta1-->4GlcNAc (LacNAc) was ineffective while terminal alpha-linked galactose (TAG) was excellent as sugar moiety in glycoproteinins for their recognition by PNA.
45 citations
TL;DR: From ricin bound to the galactomannan guar gum in a column, the nonbinding toxic A chain could be eluted by reduction with 2-mercaptoethanol and later the B chain by lactose.
31 citations
TL;DR: A lectin has been purified to homogeneity by affinity chromatography on a Sepharose-N-caproyl-d-galactosamine column from the local variety of winged bean and agglutinated native erythrocytes of all blood groups.
29 citations
Cited by
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01 Jan 1986
664 citations
TL;DR: It is demonstrated that each of these galectins mechanistically differ in their binding to glycans on the microarrays and that these differences are reflected in the determinants required for cell binding and signaling.
377 citations
TL;DR: The main results of the research performed in the last 30 years on ribosome-inactivating proteins are reviewed, with emphasis on the new, controversial and uncertain aspects.
356 citations
TL;DR: The immune recognition of nanoparticles can seriously affect the drug delivery efficacy and toxicity, and there is at present not enough knowledge on the mechanisms that dictate the nanoparticle immune recognition and stability in the biological milieu.
Abstract: Introduction: Intravenously injected nanoparticles, like any other foreign pathogen that enters the body, encounter multiple lines of defense intended to neutralize and eliminate the invading substance. Adsorption of plasma proteins on the nanoparticle surface is the first barrier of defense, which could lead to physical changes in the formulation, such as aggregation and charge neutralization, biochemical activation of defense cascades, and trigger elimination by multiple types of phagocytic cell. Areas covered: In this review, recent knowledge on the mechanisms that govern the interactions of nanoparticles (micelles, liposomes, polymeric and inorganic nanoparticles) with plasma proteins is discussed. In particular, the role of the nanoparticle surface properties and protective polymer coating in these interactions is described. The mechanisms of protein adsorption on different nanoparticles are analyzed and the implications on the clearance, toxicity and efficacy of drug delivery are discussed. The revi...
319 citations